FastQCFastQC Report
Thu 2 Feb 2017
SRR4062193_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062193_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74091
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACTTTTTTTTTTTTTT5560.7504285270815618No Hit
ACGCAGAGTACATGGGGAATAATTG3810.5142324978742357No Hit
GCGCAAGACGGACCAGAGCGAAAGC3190.4305516189550687No Hit
GTATCAACGCAGAGTACTTTTTTTT1840.24834325356656003No Hit
ACGCAGAGTACATGGGAATAACGCC1780.24024510399373744No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT1430.1930058981522722No Hit
ACGCAGAGTACATGGGTACCTGGTT1410.19030651496133133No Hit
ACCATACTCCCCCCGGAACCCAAAG1400.1889568233658609No Hit
TATCAACGCAGAGTACTTTTTTTTT1240.16736175783833393No Hit
ACGCAGAGTACATGGGAGAAATCGT1230.16601206624286352No Hit
AAGCAGGCCCGAGCCGCCTGGATAC1150.15521453347910003No Hit
TCGTAGTTCCGACCATAAACGATGC1080.145766692310807No Hit
CTCCACTCCTGGTGGTGCCCTTCCG1070.14441700071533653No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG990.1336194679515731No Hit
GATTAAGAGGGACGGCCGGGGGCAT990.1336194679515731No Hit
CTATTGGAGCTGGAATTACCGCGGC960.12957039316516178No Hit
CTCCCGACCCGGGGAGGTAGTGACG920.12417162678328002No Hit
GGCTCCACTCCTGGTGGTGCCCTTC900.12147224359233914No Hit
CCGCAGCTAGGAATAATGGAATAGG880.11877286040139828No Hit
CCAATGGATCCTCGTTAAAGGATTT830.1120244024240461No Hit
GAATAGGACCGCGGTTCTATTTTGT820.11067471082857566No Hit
GAACTACGACGGTATCTGATCGTCT820.11067471082857566No Hit
GTCAATCCTGTCCGTGTCCGGGCCG810.10932501923310524No Hit
ATACAGGACTCTTTCGAGGCCCTGT810.10932501923310524No Hit
GTATCTGATCGTCTTCGAACCTCCG810.10932501923310524No Hit
ATATTAAAGTTGCTGCAGTTAAAAA800.10797532763763479No Hit
TTCGTATTGCGCCGCTAGAGGTGAA800.10797532763763479No Hit
ATCAGATACCGTCGTAGTTCCGACC770.1039262528512235No Hit
CTCTAGATAACCTCGGGCCGATCGC760.10257656125575307No Hit
GGCGGTGACTCGCCTCGCGGCGGAC750.10122686966028262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA250.005998854518.9891951
ATAATTG659.571522E-917.54033319
ATACTCC350.002142520616.2874534
AATAATT702.3899702E-816.28745318
GGGGAAT702.3899702E-816.28745314
GTATCAA1050.016.2764551
GAGCAGA350.002152229216.2764551
CATGGGC807.3141564E-915.43914711
TACTCCC456.6499354E-414.7793545
TGGGGAA851.6281774E-814.53096213
CATGGGG2550.014.53096211
ATGGGGA1250.014.4415412
ATGGGAA602.5012017E-514.2515212
GAGCAGG400.00523627514.2418981
CTCCGGA400.00523627514.2418981
AGTACAT5150.014.20539957
GAGTACA5350.013.8519446
ACGCAGA6300.013.714421
CGCAGAG6400.013.6484852
ATGGGGC707.0340266E-613.57287712
Produced by FastQC (version 0.11.5)