##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062193_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74091
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.953786559771093	32.0	14.0	32.0	14.0	32.0
2	24.66423722179482	32.0	14.0	32.0	14.0	32.0
3	25.376509967472433	32.0	14.0	32.0	14.0	32.0
4	24.69800650551349	32.0	14.0	32.0	14.0	32.0
5	25.17583782105789	32.0	14.0	32.0	14.0	32.0
6	28.80573888866394	32.0	21.0	36.0	14.0	36.0
7	28.34947564481516	32.0	21.0	36.0	14.0	36.0
8	27.947712947591477	32.0	21.0	36.0	14.0	36.0
9	28.10130785115601	32.0	21.0	36.0	14.0	36.0
10	26.836822286107623	32.0	14.0	36.0	14.0	36.0
11	29.09600356318581	32.0	21.0	36.0	14.0	36.0
12	27.61363728388063	32.0	21.0	36.0	14.0	36.0
13	28.18915927710518	32.0	21.0	36.0	14.0	36.0
14	27.459111093115222	32.0	14.0	36.0	14.0	36.0
15	27.53423492731911	32.0	14.0	36.0	14.0	36.0
16	27.768716848200185	32.0	21.0	36.0	14.0	36.0
17	27.345939452835026	32.0	14.0	36.0	14.0	36.0
18	27.536097501720857	32.0	14.0	36.0	14.0	36.0
19	27.460150355643734	32.0	14.0	36.0	14.0	36.0
20	27.532790757311954	32.0	14.0	36.0	14.0	36.0
21	27.452173678314505	32.0	14.0	36.0	14.0	36.0
22	27.26386470691447	32.0	14.0	36.0	14.0	36.0
23	27.123213345750496	32.0	14.0	36.0	14.0	36.0
24	27.070224453712328	32.0	14.0	36.0	14.0	36.0
25	26.308040112834217	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	4.0
5	5.0
6	11.0
7	4.0
8	6.0
9	7.0
10	4.0
11	4.0
12	0.0
13	2.0
14	101.0
15	324.0
16	799.0
17	1558.0
18	2739.0
19	3654.0
20	4433.0
21	4557.0
22	4249.0
23	3903.0
24	3544.0
25	3367.0
26	3087.0
27	3005.0
28	3076.0
29	3076.0
30	3127.0
31	3575.0
32	3989.0
33	4515.0
34	6526.0
35	6838.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.10559987042961	23.571014023295678	10.574833650510858	29.74855245576386
2	14.412006856433305	18.102063678449472	32.39259829129044	35.09333117382678
3	28.45149883251677	18.52316745623625	23.832854192816942	29.192479518430037
4	11.483486077555373	12.825115739178555	28.384780466735954	47.30661771653012
5	14.429774825854528	40.47734758896269	28.721853231815974	16.37102435336681
6	42.21406100211982	31.33278425124556	13.20092353806894	13.252231208565682
7	30.341418615958077	33.55302252714602	17.28296688455513	18.82259197234077
8	32.42575260321705	30.255392137001476	17.296705968153642	20.022149291627837
9	24.905797982253556	12.692624556001242	22.300555082857258	40.10102237888795
10	15.581731483607776	28.833295060044033	27.667535695470697	27.917437760877494
11	35.924244880315555	18.88879883287405	19.579078186632085	25.60787810017831
12	23.295646418392295	26.509165079493723	26.20523834609826	23.989950156015723
13	27.449311774797046	16.926692871905015	28.717699342167467	26.906296011130472
14	27.37378103136227	16.95885896431562	23.386639293335854	32.28072071098625
15	29.766166398746403	24.049333351344778	20.11563348507977	26.068866764829046
16	30.95697980684811	22.970216789356385	21.692442763557775	24.380360640237726
17	24.4377203528347	26.00062137810858	24.175660889651354	25.385997379405367
18	25.557176432449957	23.890374692708754	25.58284031661129	24.969608558230004
19	25.179292554125414	24.088005294363935	25.349468537702087	25.383233613808564
20	25.900767070008644	22.448952031114953	25.449708297320655	26.20057260155575
21	26.28977364810113	23.382043109502458	24.901409972448814	25.4267732699476
22	25.278212954459512	23.315866241694128	24.913564907352384	26.492355896493976
23	24.175557056043214	22.63875759621877	26.36596893990547	26.819716407832544
24	25.17117968802755	23.215612127760146	25.46964683638328	26.143561347829024
25	24.858528152559998	22.636846156962847	25.315019650743487	27.189606039733665
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	5.0
2	13.0
3	21.0
4	21.0
5	21.0
6	44.5
7	68.0
8	68.0
9	68.0
10	88.5
11	109.0
12	109.0
13	109.0
14	148.5
15	188.0
16	188.0
17	188.0
18	299.5
19	411.0
20	411.0
21	411.0
22	897.0
23	1383.0
24	1383.0
25	1383.0
26	1506.0
27	1629.0
28	1629.0
29	1629.0
30	1969.0
31	2309.0
32	2309.0
33	2309.0
34	2966.0
35	3623.0
36	3623.0
37	3623.0
38	3988.0
39	4353.0
40	4353.0
41	4353.0
42	5323.0
43	6293.0
44	6293.0
45	6293.0
46	7268.0
47	8243.0
48	8243.0
49	8243.0
50	9155.0
51	10067.0
52	10067.0
53	10067.0
54	9652.5
55	9238.0
56	9238.0
57	9238.0
58	8841.5
59	8445.0
60	8445.0
61	8445.0
62	7557.5
63	6670.0
64	6670.0
65	6670.0
66	5777.0
67	4884.0
68	4884.0
69	4884.0
70	3832.0
71	2780.0
72	2780.0
73	2780.0
74	2153.0
75	1526.0
76	1526.0
77	1526.0
78	1309.5
79	1093.0
80	1093.0
81	1093.0
82	757.5
83	422.0
84	422.0
85	422.0
86	304.5
87	187.0
88	187.0
89	187.0
90	124.5
91	62.0
92	62.0
93	62.0
94	36.5
95	11.0
96	11.0
97	11.0
98	41.0
99	71.0
100	71.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.00269938319094087
5	0.020245373932056526
6	0.03779136467317218
7	0.06343550498711045
8	0.06478519658258089
9	0.06478519658258089
10	0.08368087891916696
11	0.0850305705146374
12	0.0809814957282261
13	0.0809814957282261
14	0.07153365455993306
15	0.08638026211010784
16	0.07558272934634436
17	0.08368087891916696
18	0.0769324209418148
19	0.06748457977352175
20	0.058036738605228705
21	0.06343550498711045
22	0.06343550498711045
23	0.05533735541428783
24	0.06208581339164001
25	0.06478519658258089
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	74091.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.60482379776222
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.83139878024788	60.94262461027655
2	6.537477867401141	8.97005034349651
3	1.6486326972260477	3.3931246710126732
4	0.812512295888255	2.2296905157171585
5	0.44855400354121583	1.5386484188362959
6	0.3187094235687586	1.3119002307972627
7	0.21640763328742868	1.039262528512235
8	0.1534526854219949	0.8422075555735514
9	0.11213850088530396	0.6923917884763332
>10	0.7987409010426914	10.962195138410873
>50	0.0963997639189455	4.543061910353484
>100	0.023608105449537675	2.7844137614555073
>500	0.0019673421207948064	0.7504285270815618
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGCAGAGTACTTTTTTTTTTTTTT	556	0.7504285270815618	No Hit
ACGCAGAGTACATGGGGAATAATTG	381	0.5142324978742357	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	319	0.4305516189550687	No Hit
GTATCAACGCAGAGTACTTTTTTTT	184	0.24834325356656003	No Hit
ACGCAGAGTACATGGGAATAACGCC	178	0.24024510399373744	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	143	0.1930058981522722	No Hit
ACGCAGAGTACATGGGTACCTGGTT	141	0.19030651496133133	No Hit
ACCATACTCCCCCCGGAACCCAAAG	140	0.1889568233658609	No Hit
TATCAACGCAGAGTACTTTTTTTTT	124	0.16736175783833393	No Hit
ACGCAGAGTACATGGGAGAAATCGT	123	0.16601206624286352	No Hit
AAGCAGGCCCGAGCCGCCTGGATAC	115	0.15521453347910003	No Hit
TCGTAGTTCCGACCATAAACGATGC	108	0.145766692310807	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	107	0.14441700071533653	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	99	0.1336194679515731	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	99	0.1336194679515731	No Hit
CTATTGGAGCTGGAATTACCGCGGC	96	0.12957039316516178	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	92	0.12417162678328002	No Hit
GGCTCCACTCCTGGTGGTGCCCTTC	90	0.12147224359233914	No Hit
CCGCAGCTAGGAATAATGGAATAGG	88	0.11877286040139828	No Hit
CCAATGGATCCTCGTTAAAGGATTT	83	0.1120244024240461	No Hit
GAATAGGACCGCGGTTCTATTTTGT	82	0.11067471082857566	No Hit
GAACTACGACGGTATCTGATCGTCT	82	0.11067471082857566	No Hit
GTCAATCCTGTCCGTGTCCGGGCCG	81	0.10932501923310524	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	81	0.10932501923310524	No Hit
GTATCTGATCGTCTTCGAACCTCCG	81	0.10932501923310524	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	80	0.10797532763763479	No Hit
TTCGTATTGCGCCGCTAGAGGTGAA	80	0.10797532763763479	No Hit
ATCAGATACCGTCGTAGTTCCGACC	77	0.1039262528512235	No Hit
CTCTAGATAACCTCGGGCCGATCGC	76	0.10257656125575307	No Hit
GGCGGTGACTCGCCTCGCGGCGGAC	75	0.10122686966028262	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.001349691595470435	0.0
8	0.0	0.0	0.0	0.001349691595470435	0.0
9	0.0	0.0	0.0	0.001349691595470435	0.0
10	0.0	0.0	0.0	0.001349691595470435	0.0
11	0.0	0.0	0.0	0.001349691595470435	0.0
12	0.0	0.0	0.0	0.00269938319094087	0.0
13	0.0	0.0	0.0	0.00269938319094087	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25	0.0059988545	18.989195	1
ATAATTG	65	9.571522E-9	17.540333	19
ATACTCC	35	0.0021425206	16.287453	4
AATAATT	70	2.3899702E-8	16.287453	18
GGGGAAT	70	2.3899702E-8	16.287453	14
GTATCAA	105	0.0	16.276455	1
GAGCAGA	35	0.0021522292	16.276455	1
CATGGGC	80	7.3141564E-9	15.439147	11
TACTCCC	45	6.6499354E-4	14.779354	5
TGGGGAA	85	1.6281774E-8	14.530962	13
CATGGGG	255	0.0	14.530962	11
ATGGGGA	125	0.0	14.44154	12
ATGGGAA	60	2.5012017E-5	14.25152	12
GAGCAGG	40	0.005236275	14.241898	1
CTCCGGA	40	0.005236275	14.241898	1
AGTACAT	515	0.0	14.2053995	7
GAGTACA	535	0.0	13.851944	6
ACGCAGA	630	0.0	13.71442	1
CGCAGAG	640	0.0	13.648485	2
ATGGGGC	70	7.0340266E-6	13.572877	12
>>END_MODULE