FastQCFastQC Report
Thu 2 Feb 2017
SRR4062193_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062193_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74091
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC2300.31042906695820005No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT1930.260490477925794No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG1680.2267481880390331No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC1530.20650281410697657No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG1510.20380343091603567No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA1310.176809599006627No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC1200.1619629914564522No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC1160.15656422507457046No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT1130.15251515028815915No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG1090.1471163839062774No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG920.12417162678328002No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT890.12012255199686872No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT850.11472378561498697No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG810.10932501923310524No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG810.10932501923310524No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC810.10932501923310524No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC780.10527594444669393No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT770.1039262528512235No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC750.10122686966028262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTCAC254.4024935E-544.040
AAACCTC207.8088045E-444.038
AACCTCA254.4024935E-544.039
ACACGGG301.2893727E-436.66666431
CACGGGA301.2893727E-436.66666432
CGACCCG505.8184014E-835.25
ACGGGAA250.00233860435.233
AGTGACG505.8184014E-835.219
ACCCGGG505.8184014E-835.27
GTAGTGA505.8184014E-835.217
AAGTCTG401.79722E-533.035
AGGTAGT551.3443787E-732.015
CCGGGGA551.3443787E-732.09
TGACGAA551.3443787E-732.021
CGGGGAG551.3443787E-732.010
GAGGTAG551.3443787E-732.014
GACGAAA551.3443787E-732.022
GTGACGA551.3443787E-732.020
GGTAGTG551.3443787E-732.016
GGAGGTA551.3443787E-732.013
Produced by FastQC (version 0.11.5)