##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062193_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74091
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.317164028019597	32.0	32.0	32.0	21.0	32.0
2	28.92236573942854	32.0	32.0	32.0	14.0	32.0
3	30.383082965542375	32.0	32.0	32.0	21.0	32.0
4	30.922055310361582	32.0	32.0	32.0	32.0	32.0
5	30.677842113077162	32.0	32.0	32.0	32.0	32.0
6	34.22933959590234	36.0	36.0	36.0	32.0	36.0
7	34.229447571229976	36.0	36.0	36.0	32.0	36.0
8	34.018261327286716	36.0	36.0	36.0	32.0	36.0
9	34.39317865867649	36.0	36.0	36.0	32.0	36.0
10	33.90047374175001	36.0	36.0	36.0	32.0	36.0
11	34.61433912351028	36.0	36.0	36.0	32.0	36.0
12	34.20593594363688	36.0	36.0	36.0	32.0	36.0
13	34.36059710356184	36.0	36.0	36.0	32.0	36.0
14	34.21846108164284	36.0	36.0	36.0	32.0	36.0
15	34.13653480179779	36.0	36.0	36.0	32.0	36.0
16	34.169926171869726	36.0	36.0	36.0	32.0	36.0
17	34.12322684266645	36.0	36.0	36.0	32.0	36.0
18	34.194355589747744	36.0	36.0	36.0	32.0	36.0
19	34.15603784535234	36.0	36.0	36.0	32.0	36.0
20	34.18079118921327	36.0	36.0	36.0	32.0	36.0
21	34.16987218420591	36.0	36.0	36.0	32.0	36.0
22	34.109325019233104	36.0	36.0	36.0	32.0	36.0
23	34.02141960562012	36.0	36.0	36.0	32.0	36.0
24	33.978701866623474	36.0	36.0	36.0	32.0	36.0
25	33.995397551659444	36.0	36.0	36.0	32.0	36.0
26	33.85469220283165	36.0	36.0	36.0	32.0	36.0
27	33.85585293760376	36.0	36.0	36.0	32.0	36.0
28	33.76312912499494	36.0	36.0	36.0	32.0	36.0
29	33.696616323170154	36.0	36.0	36.0	32.0	36.0
30	33.709519374822854	36.0	36.0	36.0	32.0	36.0
31	33.70244699086259	36.0	36.0	36.0	32.0	36.0
32	33.65422251015643	36.0	36.0	36.0	27.0	36.0
33	33.604405393367614	36.0	36.0	36.0	27.0	36.0
34	33.53891835715539	36.0	36.0	36.0	27.0	36.0
35	33.58588762467776	36.0	36.0	36.0	27.0	36.0
36	33.4575994385283	36.0	36.0	36.0	21.0	36.0
37	33.52049506687722	36.0	36.0	36.0	27.0	36.0
38	33.45348287916211	36.0	36.0	36.0	21.0	36.0
39	33.42126574077823	36.0	36.0	36.0	21.0	36.0
40	33.39274675736594	36.0	36.0	36.0	21.0	36.0
41	33.308229069657585	36.0	36.0	36.0	21.0	36.0
42	33.24148682026157	36.0	36.0	36.0	21.0	36.0
43	33.29826834568301	36.0	36.0	36.0	21.0	36.0
44	33.21385863330229	36.0	36.0	36.0	21.0	36.0
45	33.12402316070778	36.0	36.0	36.0	21.0	36.0
46	33.08311400844907	36.0	36.0	36.0	14.0	36.0
47	33.10491152771592	36.0	36.0	36.0	21.0	36.0
48	33.07442199457424	36.0	36.0	36.0	14.0	36.0
49	33.05516189550688	36.0	36.0	36.0	14.0	36.0
50	32.4058117720101	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	5.0
22	15.0
23	39.0
24	89.0
25	227.0
26	474.0
27	850.0
28	1474.0
29	2429.0
30	3850.0
31	5898.0
32	8839.0
33	13553.0
34	19304.0
35	17042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9881747863767	17.35579583957667	13.243969276043144	25.41206009800348
2	19.497907949790793	18.985018221082466	34.39060601970576	27.126467809420973
3	22.46021784022351	20.58009744773319	27.409536920813593	29.5501477912297
4	16.220593594363688	14.61581028734934	33.67885438177377	35.48474173651321
5	20.682674008988947	30.967323966473657	30.048183989958293	18.3018180345791
6	38.24081197446383	33.997381598304784	13.321456047293193	14.440350379938186
7	32.63824216166606	27.96021109176553	17.964395135711488	21.43715161085692
8	29.500209202197297	31.30069779055486	16.81985666275256	22.379236344495283
9	26.273096597427486	12.550782146279573	16.53777111929924	44.6383501369937
10	16.91568476603096	23.967823352363986	28.439351608157537	30.677140273447517
11	40.81602353862143	18.257278211928572	19.589423816657895	21.337274432792107
12	26.008557044715282	21.43850130245239	25.965366913660226	26.587574739172098
13	30.38155781403949	16.717280101496808	23.245738348787302	29.6554237356764
14	24.886963328879354	17.424518497523316	22.56144470988379	35.12707346371354
15	26.935795170803473	24.814079982723946	19.9862331457262	28.26389170074638
16	28.099229326098985	24.525245981293274	20.922919112982683	26.452605579625054
17	25.491625163650106	25.148803498400618	22.724757392935715	26.634813945013562
18	27.12340230257386	22.92451174906534	23.749173313897774	26.202912634463026
19	27.045120190036577	24.193221848807546	23.168805927845487	25.592852033310386
20	27.694321847457854	22.170034147197367	22.603285149343375	27.532358856001405
21	27.96965893293382	22.850278711314466	22.796291047495647	26.383771308256065
22	27.27456776126655	22.77604567356359	23.33211861089741	26.61726795427245
23	25.617146482028858	22.889419767583107	24.056902997665034	27.436530752723005
24	25.811502071776598	23.835553576007882	23.328069536111	27.02487481610452
25	25.907330175054998	22.87457316003293	23.446842396512395	27.77125426839967
26	26.12058144713933	23.27273218069671	23.89089093142217	26.71579544074179
27	27.18683780756097	22.6113832989162	23.866596482703702	26.33518241081913
28	25.498373621627458	23.0176404691528	24.804632141555654	26.67935376766409
29	26.325734569650834	23.348314910043054	23.970522735554926	26.355427784751186
30	26.12463052192574	23.099971656476495	24.891012403665762	25.884385417932005
31	26.575427514812866	23.106720114453847	23.411750415030166	26.90610195570312
32	26.84401614231148	23.51837605107233	22.875922851628403	26.761684954987786
33	25.910029558245938	23.27273218069671	23.967823352363986	26.849414908693365
34	26.482656228910784	22.98285868538264	23.823727898501822	26.710757187204752
35	27.359967606964503	22.626535294911594	23.870967741935484	26.142529356188422
36	25.13395689085044	23.788314370166415	23.36316151759323	27.714567221389913
37	27.434201646645974	23.289242812795248	23.56863274396005	25.70792279659873
38	25.901931408673118	22.71126047698101	24.102792511911026	27.28401560243484
39	26.59837227193586	23.30107570420159	22.917763291087986	27.18278873277456
40	26.842666450716013	24.070399913619738	23.89628969780405	25.190643937860198
41	25.73996841721667	23.54536988298174	24.1999703067849	26.514691393016697
42	27.245977756181837	24.203649713853796	23.493683187560737	25.056689342403626
43	26.269756644036224	22.82120152789213	24.335596377329967	26.573445450741673
44	25.99846131004603	23.435327781452038	23.388087300408966	27.178123608092967
45	26.624375759211773	24.255635038466732	23.305439330543933	25.81454987177757
46	25.53886436949157	23.683038425719722	23.70463349124725	27.073463713541457
47	26.273096597427486	23.310523545369882	24.07174960521521	26.34463025198742
48	27.49186810813729	24.285000877299538	22.940708048210983	25.282422966352186
49	26.065595896882172	24.02078553111081	23.60507490889459	26.30854366311243
50	27.09713992630485	23.232868576981737	23.46502179810768	26.20496969860573
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.5
4	2.0
5	2.5
6	3.0
7	2.0
8	1.0
9	3.0
10	5.0
11	9.5
12	14.0
13	17.0
14	20.0
15	31.5
16	43.0
17	62.0
18	81.0
19	103.0
20	125.0
21	148.0
22	171.0
23	201.5
24	232.0
25	348.5
26	465.0
27	535.5
28	606.0
29	655.0
30	704.0
31	778.0
32	852.0
33	940.0
34	1028.0
35	1203.5
36	1379.0
37	1701.0
38	2023.0
39	2224.5
40	2426.0
41	2776.0
42	3126.0
43	3122.0
44	3118.0
45	3330.0
46	3542.0
47	3843.5
48	4145.0
49	4463.0
50	4781.0
51	4650.5
52	4520.0
53	4725.0
54	4930.0
55	5378.0
56	5826.0
57	5849.0
58	5872.0
59	5432.0
60	4992.0
61	4731.5
62	4471.0
63	4001.5
64	3532.0
65	3115.0
66	2698.0
67	2415.5
68	2133.0
69	2033.0
70	1933.0
71	1581.5
72	1230.0
73	1135.5
74	1041.0
75	821.0
76	601.0
77	531.5
78	462.0
79	411.5
80	361.0
81	305.5
82	250.0
83	213.0
84	176.0
85	128.0
86	80.0
87	59.0
88	38.0
89	30.5
90	23.0
91	17.5
92	12.0
93	7.5
94	3.0
95	3.5
96	4.0
97	4.0
98	4.0
99	5.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016196299145645222
2	0.001349691595470435
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.001349691595470435
35	0.001349691595470435
36	0.0
37	0.001349691595470435
38	0.0
39	0.0
40	0.0
41	0.0
42	0.004049074786411306
43	0.00269938319094087
44	0.00269938319094087
45	0.001349691595470435
46	0.0
47	0.0
48	0.0
49	0.001349691595470435
50	0.00269938319094087
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	74091.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.39107313978755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.09885781803861	68.68040652710854
2	5.3063124351032975	8.000971777948738
3	1.373124709083748	3.105640361177471
4	0.5388664112571163	1.6250286809464036
5	0.36521178703140106	1.3766854273798437
6	0.23631350925561243	1.0689557436125847
7	0.17723513194170934	0.9353362756610115
8	0.12531777005979447	0.7558272934634436
9	0.10562497762182678	0.7166862371948011
>10	0.5943642808550252	8.679866650470368
>50	0.060868631171900174	3.0516526973586537
>100	0.01790253857997064	2.0029423276781255
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	230	0.31042906695820005	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	193	0.260490477925794	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	168	0.2267481880390331	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC	153	0.20650281410697657	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	151	0.20380343091603567	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	131	0.176809599006627	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	120	0.1619629914564522	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	116	0.15656422507457046	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	113	0.15251515028815915	No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG	109	0.1471163839062774	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	92	0.12417162678328002	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	89	0.12012255199686872	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	85	0.11472378561498697	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGACCCCCGGCCGGAGCCGGG	81	0.10932501923310524	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	81	0.10932501923310524	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC	81	0.10932501923310524	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	78	0.10527594444669393	No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT	77	0.1039262528512235	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	75	0.10122686966028262	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.001349691595470435	0.0
26	0.0	0.0	0.0	0.001349691595470435	0.0
27	0.0	0.0	0.0	0.001349691595470435	0.0
28	0.0	0.0	0.0	0.001349691595470435	0.0
29	0.0	0.0	0.0	0.001349691595470435	0.0
30	0.0	0.0	0.0	0.001349691595470435	0.0
31	0.0	0.0	0.0	0.001349691595470435	0.0
32	0.0	0.0	0.0	0.001349691595470435	0.0
33	0.0	0.0	0.0	0.00269938319094087	0.0
34	0.0	0.0	0.0	0.004049074786411305	0.0
35	0.0	0.0	0.0	0.00539876638188174	0.0
36	0.0	0.0	0.0	0.00809814957282261	0.0
37	0.0	0.0	0.0	0.009447841168293045	0.0
38	0.0	0.0	0.0	0.01079753276376348	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTCAC	25	4.4024935E-5	44.0	40
AAACCTC	20	7.8088045E-4	44.0	38
AACCTCA	25	4.4024935E-5	44.0	39
ACACGGG	30	1.2893727E-4	36.666664	31
CACGGGA	30	1.2893727E-4	36.666664	32
CGACCCG	50	5.8184014E-8	35.2	5
ACGGGAA	25	0.002338604	35.2	33
AGTGACG	50	5.8184014E-8	35.2	19
ACCCGGG	50	5.8184014E-8	35.2	7
GTAGTGA	50	5.8184014E-8	35.2	17
AAGTCTG	40	1.79722E-5	33.0	35
AGGTAGT	55	1.3443787E-7	32.0	15
CCGGGGA	55	1.3443787E-7	32.0	9
TGACGAA	55	1.3443787E-7	32.0	21
CGGGGAG	55	1.3443787E-7	32.0	10
GAGGTAG	55	1.3443787E-7	32.0	14
GACGAAA	55	1.3443787E-7	32.0	22
GTGACGA	55	1.3443787E-7	32.0	20
GGTAGTG	55	1.3443787E-7	32.0	16
GGAGGTA	55	1.3443787E-7	32.0	13
>>END_MODULE