FastQCFastQC Report
Thu 2 Feb 2017
SRR4062192_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062192_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441571
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12860.2912328934644712No Hit
TATCAACGCAGAGTACTTTTTTTTT7860.17800081980021334No Hit
GGTATCAACGCAGAGTACTTTTTTT5030.11391146610624338No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5000.11323207366425783No Hit
GATATACACTGTTCTACAAATCCCG4650.1053058285077598No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4480.10145593800317504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACGG402.7539863E-416.626195
CGGTCCA402.7539863E-416.6261910
TACCGTC807.656126E-915.4386057
CGCAAGA456.750887E-414.777162
GCGCAAG456.762509E-414.7738091
CACGAAT400.005272631514.24940514
ATCACGA400.005272631514.24940512
ACCGTCG759.61978E-713.9343318
GGTCCAA551.9539564E-413.81917211
CATCACG551.9539564E-413.81917211
TTAGAGT551.9558378E-413.8176044
TCGCCAG707.237486E-613.57086117
CGCATCG707.237486E-613.57086113
GCCGCTA500.001495770313.3009538
CGCTAGA500.001495770313.30095310
ATTAGAG500.001497049613.2994443
CTAAACA500.001497049613.2994444
GCTTTAT500.001499611113.296431
ATACCGT801.9885538E-613.0634366
ATCGCCA751.4735377E-512.66613816
Produced by FastQC (version 0.11.5)