##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062192_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441571
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.280426024353954	32.0	32.0	32.0	32.0	32.0
2	30.93455412606353	32.0	32.0	32.0	32.0	32.0
3	30.963668356844085	32.0	32.0	32.0	32.0	32.0
4	30.95515104026306	32.0	32.0	32.0	32.0	32.0
5	30.93916493610314	32.0	32.0	32.0	32.0	32.0
6	34.60087958674822	36.0	36.0	36.0	32.0	36.0
7	34.49935118021791	36.0	36.0	36.0	32.0	36.0
8	34.46760317140392	36.0	36.0	36.0	32.0	36.0
9	34.5502308801982	36.0	36.0	36.0	32.0	36.0
10	34.331776769760694	36.0	36.0	36.0	32.0	36.0
11	34.548283288531174	36.0	36.0	36.0	32.0	36.0
12	34.42869889553435	36.0	36.0	36.0	32.0	36.0
13	34.46900271983441	36.0	36.0	36.0	32.0	36.0
14	34.40324432537463	36.0	36.0	36.0	32.0	36.0
15	34.38316827871395	36.0	36.0	36.0	32.0	36.0
16	34.36167909577395	36.0	36.0	36.0	32.0	36.0
17	34.31991457772363	36.0	36.0	36.0	32.0	36.0
18	34.31653120336254	36.0	36.0	36.0	32.0	36.0
19	34.29814231459947	36.0	36.0	36.0	32.0	36.0
20	34.26544089172523	36.0	36.0	36.0	32.0	36.0
21	34.23004681013925	36.0	36.0	36.0	32.0	36.0
22	34.21967022290866	36.0	36.0	36.0	32.0	36.0
23	34.18432143415215	36.0	36.0	36.0	32.0	36.0
24	34.16750194193006	36.0	36.0	36.0	32.0	36.0
25	33.82190406525791	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	8.0
5	41.0
6	102.0
7	23.0
8	80.0
9	62.0
10	41.0
11	9.0
12	23.0
13	16.0
14	106.0
15	132.0
16	185.0
17	256.0
18	365.0
19	431.0
20	646.0
21	868.0
22	1203.0
23	1593.0
24	2303.0
25	3164.0
26	4207.0
27	5529.0
28	7532.0
29	10090.0
30	13054.0
31	18341.0
32	26112.0
33	38476.0
34	89132.0
35	217441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.36241467866638	17.71037625179475	11.715575927494418	25.211633142044448
2	16.21532259890844	19.921416762914145	38.682202142355685	25.18105849582173
3	19.546612616489078	23.826163758450058	28.52852920861028	28.09869441645058
4	12.51656244125288	15.800059341669856	36.24099965799267	35.442378559084595
5	14.30364404571765	37.05961645201119	33.626035047178895	15.010704455092263
6	33.3802446641194	35.918895521440966	17.06847241296475	13.632387401474874
7	29.563149279246193	30.774305886193904	20.379943137761696	19.282601696798213
8	27.245210979887137	34.385946615094056	19.375097775591133	18.993744629427674
9	27.11284851343262	14.624639468862807	18.82380684601012	39.43870517169445
10	16.21738075421978	27.474436398848873	31.502928843724696	24.80525400320665
11	36.80758562975831	21.635418958177887	22.37837886873241	19.17861654333139
12	24.015692677506408	24.24473320180511	29.52536453727011	22.214209583418373
13	29.8215537005667	20.080821274771925	25.352799377739682	24.744825646921694
14	22.92073382263912	20.636324265984634	25.605113449560463	30.837828461815782
15	24.8076905632308	28.10620560781575	22.75124955777901	24.334854271174446
16	24.600021767522858	26.48495319982586	25.101355028297778	23.813670004353504
17	23.015453689951133	26.5284186895543	26.194854816948038	24.261272803546525
18	23.600205873750415	25.97977086342272	27.579373626847026	22.84064963597984
19	25.1267798485216	25.39972207046171	25.701228013302536	23.772270067714153
20	24.87084569716121	25.54478293689744	25.847181953987292	23.737189411954056
21	26.111320164482184	24.803577986226657	25.044543480330596	24.04055836896056
22	24.861766979594336	25.630975078383056	25.74092672233016	23.766331219692454
23	24.13596141292522	25.38487343149344	26.04332886052078	24.435836295060565
24	23.93965497699091	25.851109650216493	26.175050438646203	24.034184934146396
25	24.304823562213958	25.49014274153513	26.080195490392093	24.12483820585882
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	516.0
1	516.0
2	412.0
3	308.0
4	308.0
5	308.0
6	606.0
7	904.0
8	904.0
9	904.0
10	977.5
11	1051.0
12	1051.0
13	1051.0
14	1291.5
15	1532.0
16	1532.0
17	1532.0
18	2493.5
19	3455.0
20	3455.0
21	3455.0
22	5619.5
23	7784.0
24	7784.0
25	7784.0
26	11538.5
27	15293.0
28	15293.0
29	15293.0
30	19913.5
31	24534.0
32	24534.0
33	24534.0
34	27623.0
35	30712.0
36	30712.0
37	30712.0
38	34400.5
39	38089.0
40	38089.0
41	38089.0
42	42534.0
43	46979.0
44	46979.0
45	46979.0
46	51617.0
47	56255.0
48	56255.0
49	56255.0
50	56867.0
51	57479.0
52	57479.0
53	57479.0
54	52952.5
55	48426.0
56	48426.0
57	48426.0
58	44097.5
59	39769.0
60	39769.0
61	39769.0
62	34736.5
63	29704.0
64	29704.0
65	29704.0
66	24122.5
67	18541.0
68	18541.0
69	18541.0
70	14076.0
71	9611.0
72	9611.0
73	9611.0
74	7389.0
75	5167.0
76	5167.0
77	5167.0
78	4175.5
79	3184.0
80	3184.0
81	3184.0
82	2236.5
83	1289.0
84	1289.0
85	1289.0
86	920.0
87	551.0
88	551.0
89	551.0
90	366.5
91	182.0
92	182.0
93	182.0
94	121.0
95	60.0
96	60.0
97	60.0
98	128.0
99	196.0
100	196.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011323207366425786
2	2.2646414732851569E-4
3	0.001358784883971094
4	0.013587848839710941
5	0.037593048456533606
6	0.0708832781138254
7	0.11436439440090042
8	0.115496715137543
9	0.1256876017673262
10	0.1385960581650516
11	0.14312534111162192
12	0.1361049525444379
13	0.13542556010245238
14	0.12183771126274144
15	0.13927545060703717
16	0.12387588858869807
17	0.13044334886122502
18	0.11866721320014222
19	0.10236179459248908
20	0.09783251164591877
21	0.09715311920393323
22	0.10598522094974534
23	0.08764162501613557
24	0.09987068897187543
25	0.09602079846729066
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	441571.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.17405553189138
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.8811994956967	51.0959338842713
2	12.44526004281598	15.724350981073712
3	3.244691735971414	6.1494100773638145
4	1.2080703754028899	3.0527481992854013
5	0.5727452993435739	1.8091321673180343
6	0.33772300092083907	1.2801198968742056
7	0.21877128463299683	0.9674468498931712
8	0.15587897778524348	0.7878005759087549
9	0.11711988195446024	0.6659044133831671
>10	0.6893276076462639	8.450314495323768
>50	0.07615585154903154	3.405373938046364
>100	0.05197366166264151	6.024331084155261
>500	7.218564119811321E-4	0.2939087380294512
>1k	3.609282059905661E-4	0.29322469907360305
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1286	0.2912328934644712	No Hit
TATCAACGCAGAGTACTTTTTTTTT	786	0.17800081980021334	No Hit
GGTATCAACGCAGAGTACTTTTTTT	503	0.11391146610624338	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	500	0.11323207366425783	No Hit
GATATACACTGTTCTACAAATCCCG	465	0.1053058285077598	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	448	0.10145593800317504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.2646414732851569E-4
13	0.0	0.0	0.0	0.0	2.2646414732851569E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGACGG	40	2.7539863E-4	16.62619	5
CGGTCCA	40	2.7539863E-4	16.62619	10
TACCGTC	80	7.656126E-9	15.438605	7
CGCAAGA	45	6.750887E-4	14.77716	2
GCGCAAG	45	6.762509E-4	14.773809	1
CACGAAT	40	0.0052726315	14.249405	14
ATCACGA	40	0.0052726315	14.249405	12
ACCGTCG	75	9.61978E-7	13.934331	8
GGTCCAA	55	1.9539564E-4	13.819172	11
CATCACG	55	1.9539564E-4	13.819172	11
TTAGAGT	55	1.9558378E-4	13.817604	4
TCGCCAG	70	7.237486E-6	13.570861	17
CGCATCG	70	7.237486E-6	13.570861	13
GCCGCTA	50	0.0014957703	13.300953	8
CGCTAGA	50	0.0014957703	13.300953	10
ATTAGAG	50	0.0014970496	13.299444	3
CTAAACA	50	0.0014970496	13.299444	4
GCTTTAT	50	0.0014996111	13.29643	1
ATACCGT	80	1.9885538E-6	13.063436	6
ATCGCCA	75	1.4735377E-5	12.666138	16
>>END_MODULE