FastQCFastQC Report
Thu 2 Feb 2017
SRR4062192_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062192_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441571
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5550.1256876017673262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATATA250.00234797635.2097851
TCGTCGT300.005742848429.33152219
CTACGAC655.920065E-727.075254
ACGGTAT756.9954694E-826.3983699
CTAGACC609.739359E-625.6650814
CAACGTA1051.10958354E-1025.14415230
TACGACG701.1301563E-625.1413065
AACGTAT1051.10958354E-1025.14130431
GATATAC2300.023.9196911
CGTATGT952.6773705E-823.15646633
CCAACGT1153.4924597E-1022.95770329
TCCTACA3150.022.3478242
GTATCAA11250.022.1039221
GGTTATG602.86339E-422.0061171
TTAGGAC904.0720988E-721.9986423
ACTGTTC2900.021.9986428
TATAATA602.8699788E-421.9986422
AATGCCG1306.730261E-1121.9986418
ACGTATG1004.6102286E-821.9986432
ACGACGG803.6088786E-621.998646
Produced by FastQC (version 0.11.5)