##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062192_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441571
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.239911588396883	32.0	32.0	32.0	32.0	32.0
2	31.47123792096854	32.0	32.0	32.0	32.0	32.0
3	31.589898340244265	32.0	32.0	32.0	32.0	32.0
4	31.676158533961697	32.0	32.0	32.0	32.0	32.0
5	31.65148300046878	32.0	32.0	32.0	32.0	32.0
6	35.31285342560992	36.0	36.0	36.0	36.0	36.0
7	35.3098550402993	36.0	36.0	36.0	36.0	36.0
8	35.26361559069776	36.0	36.0	36.0	36.0	36.0
9	35.33874280693252	36.0	36.0	36.0	36.0	36.0
10	35.23291384624443	36.0	36.0	36.0	36.0	36.0
11	35.342246207291694	36.0	36.0	36.0	36.0	36.0
12	35.271666391135284	36.0	36.0	36.0	36.0	36.0
13	35.29495596404655	36.0	36.0	36.0	36.0	36.0
14	35.273296932996054	36.0	36.0	36.0	36.0	36.0
15	35.24224643375584	36.0	36.0	36.0	36.0	36.0
16	35.251982127449494	36.0	36.0	36.0	36.0	36.0
17	35.25007982861193	36.0	36.0	36.0	36.0	36.0
18	35.230751113637446	36.0	36.0	36.0	36.0	36.0
19	35.21615549934212	36.0	36.0	36.0	36.0	36.0
20	35.2050610207645	36.0	36.0	36.0	36.0	36.0
21	35.19129652988987	36.0	36.0	36.0	36.0	36.0
22	35.16276657660942	36.0	36.0	36.0	36.0	36.0
23	35.140620647642166	36.0	36.0	36.0	36.0	36.0
24	35.11909749508007	36.0	36.0	36.0	36.0	36.0
25	35.10463322999019	36.0	36.0	36.0	36.0	36.0
26	35.06995703975125	36.0	36.0	36.0	36.0	36.0
27	35.0536176515215	36.0	36.0	36.0	36.0	36.0
28	35.01236494244414	36.0	36.0	36.0	36.0	36.0
29	34.98943091824418	36.0	36.0	36.0	36.0	36.0
30	34.967135522939685	36.0	36.0	36.0	36.0	36.0
31	34.97343122623542	36.0	36.0	36.0	36.0	36.0
32	34.92651691347484	36.0	36.0	36.0	36.0	36.0
33	34.90284914543754	36.0	36.0	36.0	36.0	36.0
34	34.88039069594697	36.0	36.0	36.0	32.0	36.0
35	34.85210305930416	36.0	36.0	36.0	32.0	36.0
36	34.82997977675164	36.0	36.0	36.0	32.0	36.0
37	34.79715832787932	36.0	36.0	36.0	32.0	36.0
38	34.775041839251216	36.0	36.0	36.0	32.0	36.0
39	34.73916085974849	36.0	36.0	36.0	32.0	36.0
40	34.72731904948468	36.0	36.0	36.0	32.0	36.0
41	34.7011397940535	36.0	36.0	36.0	32.0	36.0
42	34.647927513355725	36.0	36.0	36.0	32.0	36.0
43	34.66642057562657	36.0	36.0	36.0	32.0	36.0
44	34.59545124113676	36.0	36.0	36.0	32.0	36.0
45	34.558809342099	36.0	36.0	36.0	32.0	36.0
46	34.51400341055006	36.0	36.0	36.0	32.0	36.0
47	34.47507195898282	36.0	36.0	36.0	32.0	36.0
48	34.445237572213756	36.0	36.0	36.0	32.0	36.0
49	34.402010095771686	36.0	36.0	36.0	32.0	36.0
50	33.996455836094306	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	2.0
21	20.0
22	35.0
23	91.0
24	252.0
25	563.0
26	1174.0
27	2256.0
28	4010.0
29	5941.0
30	8770.0
31	12594.0
32	18701.0
33	30817.0
34	68749.0
35	287595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80153868459684	18.267575257806694	12.178761077985392	25.752124979611068
2	15.470205094756675	20.441986447802297	38.64052252056383	25.447285936877194
3	18.966188826233665	24.123921462055847	28.895758316914645	28.014131394795843
4	12.299069230246621	16.107978349978485	36.177276535996555	35.415675883778334
5	13.944529871753353	37.329897117337865	33.5348109364066	15.190762074502176
6	33.609016937330296	36.136594733778864	16.818209611634874	13.436178717255967
7	29.55651526028657	30.608441224627526	20.509499038659694	19.325544476426217
8	27.413485034116825	34.472146042199334	19.300859884367405	18.81350903931644
9	27.151239551510404	14.192508113078079	19.029782300015174	39.626470035396345
10	16.113829939013204	27.593524031242993	31.52109173836144	24.771554291382362
11	36.98023647386264	21.392029820798918	22.48290761848038	19.144826086858057
12	24.051851231172336	24.04845426896241	29.67246490371877	22.227229596146486
13	29.89643794542667	19.893290093778802	25.4810664649626	24.729205495831927
14	23.05948533757878	20.343274354520563	25.82914185940653	30.768098448494126
15	24.77631003847626	28.17055467863605	22.802222066213588	24.250913216674103
16	24.778233979287496	26.320461807780895	25.062447771469465	23.83885644146215
17	23.19966664477513	26.355671001945325	26.21028101936042	24.23438133391912
18	23.708638966956467	25.74795785030199	27.826228743412695	22.717174439328847
19	25.24259971782567	25.00322711409943	26.032506663707533	23.721666504367363
20	25.10791041058043	25.186493647666282	26.11703693638608	23.588559005367213
21	26.06579689336483	24.465827692488865	25.387536772115922	24.080838642030386
22	24.928267481333695	25.05395508310102	26.323966021319332	23.693811414245953
23	24.268577420165727	24.826132150888533	26.436518702541605	24.468771726404135
24	24.257075952405756	25.278214355271917	26.64720562724485	23.817504065077475
25	24.464468907604893	24.98216594839788	26.499928663793593	24.053436480203636
26	23.46931842887166	26.13074316979491	27.02641495760562	23.373523443727805
27	24.23222644705584	25.27758968587016	26.63592779384422	23.85425607322978
28	23.760983784763113	25.75210616903705	26.35791285442522	24.12899719177462
29	23.563301688528153	25.259303210377563	26.471121095731576	24.706274005362708
30	23.64533581540287	25.874895542465808	26.464839990307247	24.01492865182407
31	24.32275743370247	25.302443553683446	26.051588649591228	24.323210363022852
32	24.08756975143126	25.604889847090366	26.1717334589463	24.135806942532067
33	24.084969540503202	25.168602939511292	26.687954344724506	24.058473175261
34	24.27660706804208	25.642204431963584	26.767520070657774	23.313668429336566
35	24.48581198903911	25.476821342029577	26.670290101229703	23.367076567701613
36	23.42578396581282	26.052779973231814	26.351261071316145	24.170174989639218
37	24.309040673974092	25.7795090134976	26.065766826705318	23.845683485822992
38	24.208564421123672	25.53202089811152	26.155703159854248	24.10371152091057
39	24.681192208656896	25.117592573720408	26.12468775972842	24.076527457894272
40	25.176245722658418	25.552629135518412	26.047226833283887	23.223898308539283
41	23.92943281283616	25.571546657909934	27.172217000894545	23.326803528359356
42	24.976446288478613	26.324896273078107	25.90002355371152	22.79863388473176
43	24.047215704747842	25.432342576571514	26.57876380370877	23.94167791497187
44	23.761575725457423	26.147494389071202	25.886368217343943	24.204561668127432
45	23.861644449879744	26.291449820861583	26.040293687352513	23.80661204190616
46	23.840356193297367	25.640119757766815	25.706475706475707	24.813048342460107
47	24.338775316090196	25.679228746713413	26.109288058210872	23.872707878985523
48	24.692616221529207	25.805063537682116	25.190409218455933	24.31191102233274
49	24.213696892834495	26.164281184889937	25.60807138327747	24.013950538998095
50	24.13092612136008	26.602153265000155	25.02910123606651	24.237819377573253
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	207.0
1	132.5
2	58.0
3	134.0
4	210.0
5	205.0
6	200.0
7	157.0
8	114.0
9	151.0
10	188.0
11	301.5
12	415.0
13	753.5
14	1092.0
15	1362.0
16	1632.0
17	1671.0
18	1710.0
19	1666.0
20	1622.0
21	1826.5
22	2031.0
23	2257.5
24	2484.0
25	3074.5
26	3665.0
27	4952.0
28	6239.0
29	7350.5
30	8462.0
31	9221.0
32	9980.0
33	11548.0
34	13116.0
35	14866.5
36	16617.0
37	18124.0
38	19631.0
39	20319.0
40	21007.0
41	21895.5
42	22784.0
43	23240.5
44	23697.0
45	25263.5
46	26830.0
47	28516.0
48	30202.0
49	31282.0
50	32362.0
51	31335.0
52	30308.0
53	30323.0
54	30338.0
55	29773.5
56	29209.0
57	27942.5
58	26676.0
59	24393.5
60	22111.0
61	19389.0
62	16667.0
63	14352.5
64	12038.0
65	10186.0
66	8334.0
67	7211.0
68	6088.0
69	5478.0
70	4868.0
71	3849.5
72	2831.0
73	2477.5
74	2124.0
75	1644.0
76	1164.0
77	1017.5
78	871.0
79	745.0
80	619.0
81	488.0
82	357.0
83	290.5
84	224.0
85	174.0
86	124.0
87	78.5
88	33.0
89	21.5
90	10.0
91	9.0
92	8.0
93	6.0
94	4.0
95	4.5
96	5.0
97	4.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03510194283591993
2	0.004302818799241798
3	2.2646414732851569E-4
4	2.2646414732851569E-4
5	0.0
6	4.5292829465703137E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.5292829465703137E-4
17	0.0
18	4.5292829465703137E-4
19	0.0
20	2.2646414732851569E-4
21	0.0
22	0.0
23	0.0
24	0.0011323207366425786
25	0.0
26	6.79392441985547E-4
27	4.5292829465703137E-4
28	0.0024911056206136724
29	6.79392441985547E-4
30	9.058565893140627E-4
31	2.2646414732851569E-4
32	6.79392441985547E-4
33	2.2646414732851569E-4
34	0.001358784883971094
35	2.2646414732851569E-4
36	4.5292829465703137E-4
37	0.0024911056206136724
38	0.0
39	9.058565893140627E-4
40	0.0
41	0.001358784883971094
42	0.006114531977869923
43	0.005208675388555861
44	0.004982211241227345
45	0.002944033915270704
46	0.002944033915270704
47	9.058565893140627E-4
48	0.004982211241227345
49	0.0024911056206136724
50	0.002038177325956641
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	441571.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.96619446577492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.43485635585202	54.533824270184326
2	12.413336856352366	16.625478597833272
3	3.120966727905514	6.269977944664016
4	1.1091099101473487	2.9709147970738203
5	0.5335260438010495	1.7864104400868317
6	0.3137814159295544	1.2607648391330848
7	0.19071960487971226	0.8940236304167397
8	0.1381875356604381	0.7403114708626481
9	0.0914605716560618	0.5512289784723698
>10	0.5574270533216401	7.258294028580002
>50	0.06022233331354549	2.8334832163172305
>100	0.036065352010773856	4.148833686959816
>500	3.402391699129609E-4	0.12645409941582172
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	555	0.1256876017673262	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2646414732851569E-4
2	0.0	0.0	0.0	0.0	2.2646414732851569E-4
3	0.0	0.0	0.0	0.0	2.2646414732851569E-4
4	0.0	0.0	0.0	0.0	2.2646414732851569E-4
5	0.0	0.0	0.0	0.0	2.2646414732851569E-4
6	0.0	0.0	0.0	0.0	2.2646414732851569E-4
7	0.0	0.0	0.0	0.0	2.2646414732851569E-4
8	0.0	0.0	0.0	0.0	2.2646414732851569E-4
9	0.0	0.0	0.0	0.0	2.2646414732851569E-4
10	0.0	0.0	0.0	4.5292829465703137E-4	2.2646414732851569E-4
11	0.0	0.0	0.0	4.5292829465703137E-4	2.2646414732851569E-4
12	0.0	0.0	0.0	4.5292829465703137E-4	6.79392441985547E-4
13	0.0	0.0	0.0	4.5292829465703137E-4	6.79392441985547E-4
14	0.0	0.0	0.0	4.5292829465703137E-4	6.79392441985547E-4
15	0.0	0.0	0.0	4.5292829465703137E-4	6.79392441985547E-4
16	0.0	0.0	0.0	4.5292829465703137E-4	6.79392441985547E-4
17	0.0	0.0	0.0	6.79392441985547E-4	6.79392441985547E-4
18	0.0	0.0	0.0	6.79392441985547E-4	6.79392441985547E-4
19	0.0	0.0	0.0	6.79392441985547E-4	6.79392441985547E-4
20	0.0	0.0	0.0	0.0011323207366425783	6.79392441985547E-4
21	0.0	0.0	0.0	0.001358784883971094	6.79392441985547E-4
22	0.0	0.0	0.0	0.0015852490312996098	6.79392441985547E-4
23	0.0	0.0	0.0	0.0031704980625992195	9.058565893140627E-4
24	0.0	0.0	0.0	0.004302818799241798	9.058565893140627E-4
25	0.0	0.0	0.0	0.005435139535884376	9.058565893140627E-4
26	0.0	0.0	0.0	0.006114531977869923	9.058565893140627E-4
27	0.0	0.0	0.0	0.00837917345115508	9.058565893140627E-4
28	0.0	0.0	0.0	0.010417350777111721	9.058565893140627E-4
29	0.0	0.0	0.0	0.014267241281696489	9.058565893140627E-4
30	0.0	0.0	0.0	0.023325807174837114	9.058565893140627E-4
31	0.0	0.0	0.0	0.0346490145412629	9.058565893140627E-4
32	0.0	0.0	0.0	0.04823686338097384	9.058565893140627E-4
33	0.0	0.0	0.0	0.05956007074739963	9.058565893140627E-4
34	0.0	0.0	0.0	0.07088327811382542	9.058565893140627E-4
35	0.0	0.0	0.0	0.08854748160544963	9.058565893140627E-4
36	0.0	0.0	0.0	0.11096743219097269	9.058565893140627E-4
37	0.0	0.0	0.0	0.14538998258490707	9.058565893140627E-4
38	0.0	0.0	0.0	0.18592706495671138	9.058565893140627E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATATA	25	0.002347976	35.209785	1
TCGTCGT	30	0.0057428484	29.331522	19
CTACGAC	65	5.920065E-7	27.07525	4
ACGGTAT	75	6.9954694E-8	26.398369	9
CTAGACC	60	9.739359E-6	25.665081	4
CAACGTA	105	1.10958354E-10	25.144152	30
TACGACG	70	1.1301563E-6	25.141306	5
AACGTAT	105	1.10958354E-10	25.141304	31
GATATAC	230	0.0	23.919691	1
CGTATGT	95	2.6773705E-8	23.156466	33
CCAACGT	115	3.4924597E-10	22.957703	29
TCCTACA	315	0.0	22.347824	2
GTATCAA	1125	0.0	22.103922	1
GGTTATG	60	2.86339E-4	22.006117	1
TTAGGAC	90	4.0720988E-7	21.998642	3
ACTGTTC	290	0.0	21.998642	8
TATAATA	60	2.8699788E-4	21.998642	2
AATGCCG	130	6.730261E-11	21.99864	18
ACGTATG	100	4.6102286E-8	21.99864	32
ACGACGG	80	3.6088786E-6	21.99864	6
>>END_MODULE