FastQCFastQC Report
Thu 2 Feb 2017
SRR4062191_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062191_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237923
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22650.9519886686028673No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA15270.6418042812170324No Hit
TATCAACGCAGAGTACTTTTTTTTT15030.6317169840662735No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8250.34675083955733577No Hit
GTACTTTTTTTTTTTTTTTTTTTTT8170.34338840717374947No Hit
GGTATCAACGCAGAGTACTTTTTTT7970.3349823262147838No Hit
GTACATGGGGTGGTATCAACGCAAA7120.2992564821391795No Hit
GTATCAACGCAGAGTACATGGGGTG6830.2870676647486792No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6400.2689945906869029No Hit
TATCAACGCAGAGTACATGGGGTGG4680.19670229443979775No Hit
GGTATCAACGCAGAGTACATGGGGT3890.16349827465188316No Hit
GCAGAGTACTTTTTTTTTTTTTTTT3510.14752672082984833No Hit
ACGCAGAGTACATGGGGTGGTATCA3360.14122216011062402No Hit
CCATAGGGTCTTCTCGTCTTATTAT2810.11810543747346831No Hit
CAAGTAGAGTGATCGAAAGATGAAA2730.11474300508988201No Hit
GTATCAACGCAGAGTACATGGGTGG2560.10759783627476117No Hit
GTACATGGGTGGTATCAACGCAAAA2490.10465570793912317No Hit
ATCAACGCAGAGTACTTTTTTTTTT2470.1038150998432266No Hit
ATCCTGATGTCCTCGGATGGATTTG2430.10213388365143344No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCAC250.006011984319.0029478
TAAGTTC307.69931E-418.9989474
CAAACTT307.710222E-418.9949516
TTAATAT250.00602662718.994952
ACCTTGT250.006033959318.9909531
AAGTAGA659.93532E-917.53382
GATCGGT504.5228753E-617.1026547
TAACCTA453.520239E-516.8879535
TGACAAC402.754367E-416.6205817
TCTACAC350.002163730816.2848133
AAGTTCC350.002163730816.2848135
AGTTCCT350.002163730816.2848136
AACCTAT350.002163730816.2848136
AAACTTT350.002166776716.28138517
CTGACAA350.002166776716.28138516
CTATTCT508.637518E-515.205569
CAAGTAG755.8078513E-815.1927621
ACCTATT456.724625E-414.7800697
TATTCTC456.724625E-414.78006910
ATCAGGA456.7461375E-414.77384952
Produced by FastQC (version 0.11.5)