##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062191_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 237923 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.84231032729076 32.0 32.0 32.0 32.0 32.0 2 30.533101045296167 32.0 32.0 32.0 32.0 32.0 3 30.616422119761435 32.0 32.0 32.0 32.0 32.0 4 30.557672019939226 32.0 32.0 32.0 32.0 32.0 5 30.59013210156227 32.0 32.0 32.0 32.0 32.0 6 34.12433014042358 36.0 36.0 36.0 32.0 36.0 7 33.983885542801666 36.0 36.0 36.0 32.0 36.0 8 33.871933356590155 36.0 36.0 36.0 32.0 36.0 9 33.898542805865766 36.0 36.0 36.0 32.0 36.0 10 33.7511421762503 36.0 36.0 36.0 32.0 36.0 11 34.10877048456854 36.0 36.0 36.0 32.0 36.0 12 33.92007918528263 36.0 36.0 36.0 32.0 36.0 13 34.01494180890456 36.0 36.0 36.0 32.0 36.0 14 33.90197248689702 36.0 36.0 36.0 32.0 36.0 15 33.92212186295566 36.0 36.0 36.0 32.0 36.0 16 33.89732812716719 36.0 36.0 36.0 32.0 36.0 17 33.811270873349784 36.0 36.0 36.0 32.0 36.0 18 33.863346544890575 36.0 36.0 36.0 32.0 36.0 19 33.79758157050811 36.0 36.0 36.0 32.0 36.0 20 33.683485833652064 36.0 36.0 36.0 27.0 36.0 21 33.66779168050167 36.0 36.0 36.0 27.0 36.0 22 33.673318678732194 36.0 36.0 36.0 27.0 36.0 23 33.712902913967966 36.0 36.0 36.0 27.0 36.0 24 33.72158639559858 36.0 36.0 36.0 27.0 36.0 25 33.4111035923387 36.0 36.0 36.0 21.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 10.0 6 56.0 7 19.0 8 36.0 9 33.0 10 11.0 11 5.0 12 10.0 13 10.0 14 123.0 15 152.0 16 202.0 17 251.0 18 357.0 19 472.0 20 763.0 21 1002.0 22 1344.0 23 1779.0 24 2358.0 25 2754.0 26 3427.0 27 4105.0 28 5220.0 29 6520.0 30 8223.0 31 10918.0 32 15686.0 33 21518.0 34 46835.0 35 103719.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.664172831203764 19.424176193678548 11.940568258238063 23.971082716879625 2 15.91523307960979 20.726873820521767 39.13408960041694 24.223803499451506 3 18.39953933901874 26.752802424354506 29.093515019817666 25.754143216809084 4 12.066581198377504 16.60746936802505 35.956789474790355 35.36915995880709 5 13.047371983804307 38.98402714418456 32.558726207844735 15.409874664166399 6 30.140679213542214 38.28493323520134 17.30459467984439 14.269792871412049 7 27.78635654087442 33.54315071086857 19.544152111988623 19.126340636268395 8 27.61719637492742 33.15788587921474 18.487196963959644 20.737720781898197 9 27.98943001169768 14.299359573160983 19.627694042599746 38.083516372541595 10 16.894123984227384 26.82860112697631 29.419636657450543 26.857638231345764 11 34.95919561273922 23.97967987811294 21.544463945251835 19.51666056389601 12 24.0644411898389 25.656111644193054 28.366412470750635 21.913034695217412 13 28.779079554253777 21.403561929771403 26.256186243813755 23.56117227216106 14 23.31714788228661 20.677264350211644 26.27470946133585 29.7308783061659 15 25.65696441197266 26.846318305780954 23.81064610619171 23.68607117605468 16 24.165804068099 28.332786319607493 23.67559561379148 23.825813998502024 17 21.19132282702464 27.66764155111831 25.58503587434511 25.555999747511944 18 21.6622978823668 27.334003727842273 27.717306697072846 23.286391692718087 19 24.554281339133215 25.518918327682094 27.366578883158187 22.560221450026503 20 25.222124995793088 24.60833641840272 26.92844882711271 23.241089758691484 21 26.28767100237258 24.107338168234364 26.589712093422403 23.015278735970654 22 24.61671154493437 25.116543251430496 26.744362167620327 23.52238303601481 23 23.498904278990338 25.503169388793783 27.69587327492292 23.30205305729296 24 23.90115437687208 26.453909063372933 26.67308922020664 22.971847339548347 25 23.529733458975567 26.17032375311301 27.24305041394629 23.056892373965134 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1661.0 1 1661.0 2 998.0 3 335.0 4 335.0 5 335.0 6 777.0 7 1219.0 8 1219.0 9 1219.0 10 1188.5 11 1158.0 12 1158.0 13 1158.0 14 1038.0 15 918.0 16 918.0 17 918.0 18 1559.5 19 2201.0 20 2201.0 21 2201.0 22 3307.0 23 4413.0 24 4413.0 25 4413.0 26 6546.0 27 8679.0 28 8679.0 29 8679.0 30 10687.5 31 12696.0 32 12696.0 33 12696.0 34 14072.5 35 15449.0 36 15449.0 37 15449.0 38 17051.0 39 18653.0 40 18653.0 41 18653.0 42 21317.0 43 23981.0 44 23981.0 45 23981.0 46 28234.5 47 32488.0 48 32488.0 49 32488.0 50 33093.5 51 33699.0 52 33699.0 53 33699.0 54 31049.5 55 28400.0 56 28400.0 57 28400.0 58 24777.0 59 21154.0 60 21154.0 61 21154.0 62 17307.5 63 13461.0 64 13461.0 65 13461.0 66 11079.0 67 8697.0 68 8697.0 69 8697.0 70 6676.5 71 4656.0 72 4656.0 73 4656.0 74 3502.5 75 2349.0 76 2349.0 77 2349.0 78 1697.0 79 1045.0 80 1045.0 81 1045.0 82 692.0 83 339.0 84 339.0 85 339.0 86 228.5 87 118.0 88 118.0 89 118.0 90 78.5 91 39.0 92 39.0 93 39.0 94 25.5 95 12.0 96 12.0 97 12.0 98 57.5 99 103.0 100 103.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0012609121438448573 2 0.0 3 0.0016812161917931434 4 0.007565472863069144 5 0.03446493193175943 6 0.062204999096346296 7 0.10759783627476117 8 0.10297449174733002 9 0.11306178889808886 10 0.12440999819269259 11 0.1370191196311412 12 0.13071455891191688 13 0.12567091033653746 14 0.10969935651450259 15 0.13239577510371003 16 0.11306178889808886 17 0.1210475658091063 18 0.10591662008296802 19 0.09120597840477801 20 0.09204658650067458 21 0.08700293792529515 22 0.10213388365143344 23 0.07565472863069145 24 0.09204658650067458 25 0.0886841541170883 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 237923.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.09949437423032 #Duplication Level Percentage of deduplicated Percentage of total 1 62.31647824517691 22.4959335583361 2 14.949527879006624 10.793407951311979 3 7.387441930864255 8.00048755269562 4 4.281107010210854 6.181831937223388 5 2.598702977098348 4.69059317510287 6 1.815133486243873 3.9315240645082654 7 1.259765511299468 3.183382859160316 8 0.9605420950296312 2.774006716458686 9 0.7672693825751842 2.4928233083812827 >10 3.363643772776491 21.4661886408628 >50 0.20840852728521694 5.238249349579486 >100 0.08150054139645356 4.645620641972402 >500 0.006985760691124591 1.8804403105206307 >1k 0.0034928803455622956 2.2255099338861735 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2265 0.9519886686028673 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1527 0.6418042812170324 No Hit TATCAACGCAGAGTACTTTTTTTTT 1503 0.6317169840662735 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 825 0.34675083955733577 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 817 0.34338840717374947 No Hit GGTATCAACGCAGAGTACTTTTTTT 797 0.3349823262147838 No Hit GTACATGGGGTGGTATCAACGCAAA 712 0.2992564821391795 No Hit GTATCAACGCAGAGTACATGGGGTG 683 0.2870676647486792 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 640 0.2689945906869029 No Hit TATCAACGCAGAGTACATGGGGTGG 468 0.19670229443979775 No Hit GGTATCAACGCAGAGTACATGGGGT 389 0.16349827465188316 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 351 0.14752672082984833 No Hit ACGCAGAGTACATGGGGTGGTATCA 336 0.14122216011062402 No Hit CCATAGGGTCTTCTCGTCTTATTAT 281 0.11810543747346831 No Hit CAAGTAGAGTGATCGAAAGATGAAA 273 0.11474300508988201 No Hit GTATCAACGCAGAGTACATGGGTGG 256 0.10759783627476117 No Hit GTACATGGGTGGTATCAACGCAAAA 249 0.10465570793912317 No Hit ATCAACGCAGAGTACTTTTTTTTTT 247 0.1038150998432266 No Hit ATCCTGATGTCCTCGGATGGATTTG 243 0.10213388365143344 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 4.203040479482858E-4 3 0.0 0.0 0.0 0.0 4.203040479482858E-4 4 0.0 0.0 0.0 0.0 4.203040479482858E-4 5 0.0 0.0 0.0 0.0 4.203040479482858E-4 6 0.0 0.0 0.0 0.0 4.203040479482858E-4 7 0.0 0.0 0.0 0.0 4.203040479482858E-4 8 0.0 0.0 0.0 0.0 4.203040479482858E-4 9 0.0 0.0 0.0 0.0 4.203040479482858E-4 10 0.0 0.0 0.0 0.0 4.203040479482858E-4 11 0.0 0.0 0.0 0.0 4.203040479482858E-4 12 0.0 0.0 0.0 0.0 4.203040479482858E-4 13 0.0 0.0 0.0 0.0 4.203040479482858E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTGCAC 25 0.0060119843 19.002947 8 TAAGTTC 30 7.69931E-4 18.998947 4 CAAACTT 30 7.710222E-4 18.99495 16 TTAATAT 25 0.006026627 18.99495 2 ACCTTGT 25 0.0060339593 18.990953 1 AAGTAGA 65 9.93532E-9 17.5338 2 GATCGGT 50 4.5228753E-6 17.102654 7 TAACCTA 45 3.520239E-5 16.887953 5 TGACAAC 40 2.754367E-4 16.62058 17 TCTACAC 35 0.0021637308 16.284813 3 AAGTTCC 35 0.0021637308 16.284813 5 AGTTCCT 35 0.0021637308 16.284813 6 AACCTAT 35 0.0021637308 16.284813 6 AAACTTT 35 0.0021667767 16.281385 17 CTGACAA 35 0.0021667767 16.281385 16 CTATTCT 50 8.637518E-5 15.20556 9 CAAGTAG 75 5.8078513E-8 15.192762 1 ACCTATT 45 6.724625E-4 14.780069 7 TATTCTC 45 6.724625E-4 14.780069 10 ATCAGGA 45 6.7461375E-4 14.7738495 2 >>END_MODULE