FastQCFastQC Report
Thu 2 Feb 2017
SRR4062191_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062191_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237923
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.45687050011978664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6630.27866158378971345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.23915300328257463No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4200.17652770013828004No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA3790.15929523417240032No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3040.12777243057627888No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAA3030.12735212652833058No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA2980.12525060628858917No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.12020695771320974No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG2790.11726482937757174No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA2590.10885874841860602No Hit
CATCAGGATCGGTCGATGGTGCAGGTTACCCTTCCCACCTCCGTTCACTT2470.1038150998432266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGG250.002349283835.19915835
CCTTAAG250.002349283835.19915834
TCGTTAG250.002349283835.19915814
ACCGTGT250.002349283835.1991588
GCACTGA250.002349283835.19915811
TAATACA300.00573653229.332634
ATAGCGC300.00573653229.3326323
TCTAGTC300.00573653229.3326311
GCGCACA300.00573653229.3326326
GCACACG300.00573653229.332637
ACGGCTC300.00573653229.332638
TAGGCAC300.00573653229.332634
ATTTGAG852.6193447E-1028.46990821
GAATTAT554.9158207E-628.0169961
ACACGGC407.0164073E-427.4993446
TCAGGGC407.0164073E-427.49934434
ACCCGCC407.0164073E-427.4993447
GTCTAAC756.921982E-826.4160231
ACTGTTC508.315165E-526.3993688
TCTAACA801.2982673E-724.7546122
Produced by FastQC (version 0.11.5)