##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062191_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	237923
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06884580305393	32.0	32.0	32.0	32.0	32.0
2	31.368703319981673	32.0	32.0	32.0	32.0	32.0
3	31.511568868919777	32.0	32.0	32.0	32.0	32.0
4	31.639837258272635	32.0	32.0	32.0	32.0	32.0
5	31.617325773464525	32.0	32.0	32.0	32.0	32.0
6	35.22303434304376	36.0	36.0	36.0	36.0	36.0
7	35.2161623718598	36.0	36.0	36.0	36.0	36.0
8	35.17871328118761	36.0	36.0	36.0	36.0	36.0
9	35.202636987596826	36.0	36.0	36.0	36.0	36.0
10	35.118777923950184	36.0	36.0	36.0	36.0	36.0
11	35.23593347427529	36.0	36.0	36.0	36.0	36.0
12	35.156117735569914	36.0	36.0	36.0	36.0	36.0
13	35.19411742454491	36.0	36.0	36.0	36.0	36.0
14	35.1243679677879	36.0	36.0	36.0	36.0	36.0
15	35.15581511665539	36.0	36.0	36.0	36.0	36.0
16	35.155163645381066	36.0	36.0	36.0	36.0	36.0
17	35.124973205616946	36.0	36.0	36.0	36.0	36.0
18	35.134001336566875	36.0	36.0	36.0	36.0	36.0
19	35.08504852410233	36.0	36.0	36.0	36.0	36.0
20	35.039504377466656	36.0	36.0	36.0	36.0	36.0
21	35.029778541797135	36.0	36.0	36.0	36.0	36.0
22	35.03138830630078	36.0	36.0	36.0	36.0	36.0
23	35.050583592170575	36.0	36.0	36.0	36.0	36.0
24	35.0232722351349	36.0	36.0	36.0	36.0	36.0
25	35.021065638883165	36.0	36.0	36.0	36.0	36.0
26	34.98993371805164	36.0	36.0	36.0	36.0	36.0
27	35.007178793138955	36.0	36.0	36.0	36.0	36.0
28	34.97494987874228	36.0	36.0	36.0	36.0	36.0
29	34.96415226775049	36.0	36.0	36.0	36.0	36.0
30	34.94039247991997	36.0	36.0	36.0	36.0	36.0
31	34.91838115692892	36.0	36.0	36.0	36.0	36.0
32	34.85556251392257	36.0	36.0	36.0	32.0	36.0
33	34.80127184004909	36.0	36.0	36.0	32.0	36.0
34	34.76333519668128	36.0	36.0	36.0	32.0	36.0
35	34.757093681569245	36.0	36.0	36.0	32.0	36.0
36	34.727046145181426	36.0	36.0	36.0	32.0	36.0
37	34.706064567107845	36.0	36.0	36.0	32.0	36.0
38	34.68251072826082	36.0	36.0	36.0	32.0	36.0
39	34.63306616006019	36.0	36.0	36.0	32.0	36.0
40	34.61681300252603	36.0	36.0	36.0	32.0	36.0
41	34.54478549782913	36.0	36.0	36.0	32.0	36.0
42	34.484984637887045	36.0	36.0	36.0	32.0	36.0
43	34.44422355131702	36.0	36.0	36.0	32.0	36.0
44	34.353173085409985	36.0	36.0	36.0	32.0	36.0
45	34.25853322293347	36.0	36.0	36.0	32.0	36.0
46	34.15971133517987	36.0	36.0	36.0	32.0	36.0
47	34.050112851636875	36.0	36.0	36.0	32.0	36.0
48	33.963290644452194	36.0	36.0	36.0	32.0	36.0
49	33.85510017946983	36.0	36.0	36.0	32.0	36.0
50	33.48082362781236	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	3.0
21	12.0
22	23.0
23	77.0
24	171.0
25	404.0
26	795.0
27	1576.0
28	2716.0
29	4084.0
30	5874.0
31	8234.0
32	11917.0
33	17891.0
34	36533.0
35	147610.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.21400794568348	19.682593067496267	12.68702835642066	24.416370630399598
2	15.574573257617471	20.674885564527315	39.046350044345054	24.704191133510154
3	18.147888182311085	26.571201607242678	29.620087171059545	25.660823039386692
4	11.749179356346382	16.431786754538233	36.36638744467748	35.45264644443791
5	12.6389630258529	38.73059771438659	32.78077361163065	15.849665648129857
6	30.341328917338807	38.086271608881866	16.992472354501245	14.579927119278086
7	27.830432534895742	32.86987806979569	20.20569680106589	19.093992594242675
8	27.760662062936326	32.534475439532955	18.87879692169315	20.82606557583756
9	27.972495303102264	13.447207710057455	20.167449132702597	38.41284785413768
10	16.88116273400526	26.210270592883383	30.07834500382478	26.830221669286576
11	35.27107509572425	23.249538716307377	21.955422552674605	19.52396363529377
12	24.123350831991864	25.286752436712717	28.85681501998546	21.73308171130996
13	28.70676647486792	20.834471656796527	27.066319775725763	23.392442092609794
14	23.34242591090395	19.863569306035984	26.944011297772807	29.849993485287257
15	25.626778411502883	26.0996204654447	24.52642241397427	23.74717870907815
16	24.196585435562916	27.411504610754783	24.36008439740755	24.031825556274747
17	21.106408375819065	26.664929409935144	26.71536589568894	25.513296318556844
18	21.903304850729015	25.642329661276968	29.031661503931943	23.42270398406207
19	24.41798396960361	24.355358666459317	29.05730005085679	22.169357313080283
20	24.912786543489045	23.51484940442666	28.578273551836315	22.994090500247978
21	25.78439242948349	22.79519004047528	28.38061053366005	23.03980699638118
22	24.245239005896867	24.044753975025536	28.317985230515756	23.392021788561845
23	23.28064121585555	24.351996234075727	29.277119067933743	23.090243482134976
24	23.95899444769018	24.939159966206986	28.194469546358214	22.90737603974462
25	23.718277418649812	24.608064827968832	28.800195021897935	22.873462731483425
26	23.115461725011873	24.878637206154934	29.104794408274948	22.90110666055825
27	22.406408796123113	25.380900543453134	29.048893969897826	23.163796690525924
28	22.237726967047745	24.9936953597848	29.195107599193005	23.573470073974445
29	22.46230923450024	25.268259058602993	28.76014508895735	23.509286617939416
30	22.361109943594958	25.365455905717	28.960331537226487	23.313102613461556
31	23.109577468340596	25.25186720073301	28.463410431105864	23.17514489982053
32	22.410285723892702	25.092257126285084	28.800615327712443	23.696841822109768
33	23.26593057417736	25.000525380059933	28.47307742420867	23.26046662155403
34	23.060175436384345	24.919195867535862	28.916741271262307	23.103887424817483
35	23.2192768248551	25.500687197118392	27.90398574328669	23.37605023473981
36	22.8267128440714	25.78817516591502	28.069165234130374	23.315946755883203
37	23.241845998655013	25.9406523201076	27.467215870880967	23.35028581035642
38	23.14824544075184	25.927716109833852	27.882129932793383	23.041908516620925
39	22.595640588091896	26.104773833441214	27.806592076394786	23.492993502072107
40	22.64051815083031	25.618792634591863	28.5777331321478	23.162956082430032
41	23.182387505148746	26.219097014988108	27.85198510436193	22.746530375501216
42	22.657576623682306	26.941861833588327	27.329813883891795	23.070747658837572
43	23.248512054877434	26.295436968290797	26.742240828541647	23.713810148290122
44	22.850465718416892	25.994485355929925	27.65560375264804	23.499445173005146
45	23.550427673749027	26.148834667843555	26.675073030283926	23.625664628123488
46	23.570750077756575	26.768436714553513	26.133354628827938	23.527458578861978
47	23.174273597316734	27.457243851899175	25.969342507323923	23.39914004346017
48	23.662453712849437	27.387702434042122	25.412643905227455	23.537199947880982
49	23.73870901303429	26.947892279045526	26.039569418816278	23.273829289103908
50	23.05645690075489	27.1927907328637	26.189075135762202	23.56167723061921
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	752.0
1	399.5
2	47.0
3	145.0
4	243.0
5	228.5
6	214.0
7	157.5
8	101.0
9	173.5
10	246.0
11	404.5
12	563.0
13	1117.5
14	1672.0
15	2208.5
16	2745.0
17	2874.0
18	3003.0
19	2906.0
20	2809.0
21	2744.0
22	2679.0
23	2403.5
24	2128.0
25	2097.5
26	2067.0
27	2583.0
28	3099.0
29	3399.0
30	3699.0
31	4103.0
32	4507.0
33	5487.5
34	6468.0
35	6759.0
36	7050.0
37	8079.0
38	9108.0
39	9596.5
40	10085.0
41	11263.0
42	12441.0
43	12514.5
44	12588.0
45	13384.0
46	14180.0
47	14834.5
48	15489.0
49	16312.0
50	17135.0
51	17492.5
52	17850.0
53	17314.0
54	16778.0
55	16222.5
56	15667.0
57	14924.0
58	14181.0
59	12922.5
60	11664.0
61	9858.5
62	8053.0
63	6852.0
64	5651.0
65	5128.0
66	4605.0
67	3701.5
68	2798.0
69	2525.0
70	2252.0
71	1806.0
72	1360.0
73	1092.5
74	825.0
75	666.5
76	508.0
77	397.5
78	287.0
79	225.0
80	163.0
81	112.5
82	62.0
83	61.5
84	61.0
85	43.5
86	26.0
87	14.0
88	2.0
89	2.5
90	3.0
91	1.5
92	0.0
93	1.5
94	3.0
95	3.0
96	3.0
97	3.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024377634781000575
2	0.006724864767172573
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	4.2030404794828584E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.2030404794828584E-4
17	0.0
18	0.0
19	0.0
20	4.2030404794828584E-4
21	0.0
22	0.0
23	0.0
24	0.0016812161917931434
25	4.2030404794828584E-4
26	0.0
27	0.0
28	0.0012609121438448573
29	0.0
30	4.2030404794828584E-4
31	0.0
32	4.2030404794828584E-4
33	0.0
34	8.406080958965717E-4
35	0.0
36	0.0
37	0.0012609121438448573
38	0.0
39	4.2030404794828584E-4
40	0.0
41	4.2030404794828584E-4
42	0.0029421283356380005
43	0.004623344527431144
44	0.004623344527431144
45	0.0033624323835862867
46	4.2030404794828584E-4
47	0.0016812161917931434
48	0.0025218242876897147
49	0.005043648575379429
50	0.0029421283356380005
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	237923.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.71346191835174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.420499504062235	23.163796690525924
2	15.370727412542209	11.593666858605516
3	7.719912180008692	8.734338420413328
4	4.242775468354712	6.400390042156497
5	2.7282149583746618	5.1445215468870185
6	1.9514315327263203	4.41571432774469
7	1.3128420020283298	3.465827179381565
8	0.9484113274415183	2.8614299584319296
9	0.7411204850159926	2.5155197269704903
>10	3.298822008492238	21.37960600698545
>50	0.19168830589887328	5.072649554687861
>100	0.07021141436993614	4.277854600017653
>500	0.0022289337895217824	0.5178145870722881
>1k	0.0011144668947608912	0.45687050011978664
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1087	0.45687050011978664	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	663	0.27866158378971345	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	569	0.23915300328257463	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	420	0.17652770013828004	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	379	0.15929523417240032	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	304	0.12777243057627888	No Hit
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAAAA	303	0.12735212652833058	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	298	0.12525060628858917	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	286	0.12020695771320974	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	279	0.11726482937757174	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	259	0.10885874841860602	No Hit
CATCAGGATCGGTCGATGGTGCAGGTTACCCTTCCCACCTCCGTTCACTT	247	0.1038150998432266	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	8.406080958965716E-4	0.0
24	0.0	0.0	0.0	0.002101520239741429	0.0
25	0.0	0.0	0.0	0.0029421283356380005	0.0
26	0.0	0.0	0.0	0.005884256671276001	0.0
27	0.0	0.0	0.0	0.0067248647671725725	0.0
28	0.0	0.0	0.0	0.0067248647671725725	0.0
29	0.0	0.0	0.0	0.007565472863069144	0.0
30	0.0	0.0	0.0	0.014710641678190002	0.0
31	0.0	0.0	0.0	0.027319763116638578	0.0
32	0.0	0.0	0.0	0.042450708842776866	0.0
33	0.0	0.0	0.0	0.05379891813738058	0.0
34	0.0	0.0	0.0	0.06346591124019116	0.0
35	0.0	0.0	0.0	0.08069837720607087	0.0
36	0.0	0.0	0.0	0.09330749864451944	0.0
37	0.0	0.0	0.0	0.12062726176115803	0.0
38	0.0	0.0	0.0	0.14920793702164145	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGG	25	0.0023492838	35.199158	35
CCTTAAG	25	0.0023492838	35.199158	34
TCGTTAG	25	0.0023492838	35.199158	14
ACCGTGT	25	0.0023492838	35.199158	8
GCACTGA	25	0.0023492838	35.199158	11
TAATACA	30	0.005736532	29.33263	4
ATAGCGC	30	0.005736532	29.33263	23
TCTAGTC	30	0.005736532	29.33263	11
GCGCACA	30	0.005736532	29.33263	26
GCACACG	30	0.005736532	29.33263	7
ACGGCTC	30	0.005736532	29.33263	8
TAGGCAC	30	0.005736532	29.33263	4
ATTTGAG	85	2.6193447E-10	28.469908	21
GAATTAT	55	4.9158207E-6	28.016996	1
ACACGGC	40	7.0164073E-4	27.499344	6
TCAGGGC	40	7.0164073E-4	27.499344	34
ACCCGCC	40	7.0164073E-4	27.499344	7
GTCTAAC	75	6.921982E-8	26.416023	1
ACTGTTC	50	8.315165E-5	26.399368	8
TCTAACA	80	1.2982673E-7	24.754612	2
>>END_MODULE