FastQCFastQC Report
Thu 2 Feb 2017
SRR4062190_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062190_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415968
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38720.1602670234042835No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG350.002168215816.288479
CCGATCA653.3680735E-614.6178579
GGTATCA8300.013.3852281
CGATCAA957.380913E-813.0016610
GTATCAA23350.012.8911121
CCGATAA1551.8189894E-1212.2601389
ATAACGA1603.6379788E-1211.8762712
CGGAATT802.8671933E-511.87528615
CGCAAGA5150.011.8036622
TAACGAA1552.5465852E-1111.64568213
TTAGAGT2350.011.3187164
CGTCGTA3500.011.12999210
TCTTATA2150.011.0444872
TAGACTG2500.011.02049355
GATAACG1900.011.00186111
ACTCTAA3300.010.9407910
CGATAAC1658.0035534E-1110.9407910
CAAGACG5300.010.9335454
AAGACGG5500.010.8823055
CGCCGGT4050.010.7915017
Produced by FastQC (version 0.11.5)