##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062190_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2415968
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.217929210982927	32.0	32.0	32.0	32.0	32.0
2	30.68784768672433	32.0	32.0	32.0	32.0	32.0
3	30.72163000503318	32.0	32.0	32.0	32.0	32.0
4	30.726025344706553	32.0	32.0	32.0	32.0	32.0
5	30.618119941985988	32.0	32.0	32.0	32.0	32.0
6	34.28800174505623	36.0	36.0	36.0	32.0	36.0
7	34.21537661094849	36.0	36.0	36.0	32.0	36.0
8	34.15647475463251	36.0	36.0	36.0	32.0	36.0
9	34.322293176068555	36.0	36.0	36.0	32.0	36.0
10	33.94058116663797	36.0	36.0	36.0	32.0	36.0
11	34.31398470509543	36.0	36.0	36.0	32.0	36.0
12	34.09449835428284	36.0	36.0	36.0	32.0	36.0
13	34.208977933482565	36.0	36.0	36.0	32.0	36.0
14	34.09445779083167	36.0	36.0	36.0	32.0	36.0
15	34.04162141220414	36.0	36.0	36.0	32.0	36.0
16	34.03994754897416	36.0	36.0	36.0	32.0	36.0
17	33.9725004635823	36.0	36.0	36.0	32.0	36.0
18	33.97683288851508	36.0	36.0	36.0	32.0	36.0
19	33.98415334971324	36.0	36.0	36.0	32.0	36.0
20	33.972314202837126	36.0	36.0	36.0	32.0	36.0
21	33.95386818037325	36.0	36.0	36.0	32.0	36.0
22	33.92124398998662	36.0	36.0	36.0	32.0	36.0
23	33.88319257539835	36.0	36.0	36.0	32.0	36.0
24	33.861411657770304	36.0	36.0	36.0	32.0	36.0
25	33.49031609690194	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	30.0
5	203.0
6	520.0
7	101.0
8	418.0
9	310.0
10	167.0
11	38.0
12	93.0
13	102.0
14	397.0
15	564.0
16	848.0
17	1218.0
18	1633.0
19	2320.0
20	3579.0
21	5165.0
22	7719.0
23	11463.0
24	16199.0
25	22920.0
26	31118.0
27	39906.0
28	52353.0
29	68801.0
30	89437.0
31	119972.0
32	168241.0
33	235872.0
34	502184.0
35	1032074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.403720203663745	17.14080174904789	11.646177082820248	25.809300964468118
2	16.92478988727072	19.026807093645807	37.331128555256385	26.717274463827085
3	19.432621412750994	22.52783891077351	27.9771584960469	30.062381180428595
4	13.074913793460308	15.155461541327394	34.903941284342906	36.86568338086939
5	15.138944211147834	36.16064811095994	33.24185493026911	15.458552747623122
6	35.46681632179718	34.474992130680405	16.217673663458193	13.840517884064212
7	31.25203552525046	29.891330920674804	19.766617965991294	19.090015588083446
8	28.49964240104288	33.13794535158649	18.58018711168607	19.782225135684563
9	26.942188830619347	14.66995978227312	18.273517838975764	40.11433354813177
10	16.33437667858114	26.574509108271382	30.904585303421158	26.18652890972632
11	37.38014905205216	21.32376957448043	21.68052458364075	19.61555678982666
12	24.618548565583453	23.473759075713353	28.273328696868894	23.6343636618343
13	29.38606925008227	19.418843389512443	24.968854620913284	26.226232739492012
14	23.582446671849333	19.309357144869907	24.756171545169593	32.35202463811117
15	25.235351508263932	26.987318331513848	21.909926085078833	25.867404075143384
16	25.977294907205	26.01807207696276	23.08199153376445	24.922641482067785
17	24.283281606082447	26.109656246205414	24.726634360791667	24.88042778692047
18	25.00105666281848	25.35336047782707	25.316563749089404	24.329019110265044
19	25.63916334938665	25.159096860157177	24.969713592506537	24.232026197949637
20	25.734397902679074	24.631713527438265	24.61870470215176	25.015183867730904
21	26.4565667981776	24.30534179102066	24.465342387604966	24.772749023196774
22	25.972704405173726	24.162306821246958	24.789900820508613	25.075087953070703
23	24.6312375208159	24.41263949760151	25.26955037655032	25.68657260503227
24	24.79906006385832	24.900021998681737	24.953713696478676	25.347204240981263
25	25.033525433966393	24.553335992997045	24.937081553492177	25.476057019544385
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	343.0
1	343.0
2	557.0
3	771.0
4	771.0
5	771.0
6	1628.5
7	2486.0
8	2486.0
9	2486.0
10	3051.0
11	3616.0
12	3616.0
13	3616.0
14	4984.5
15	6353.0
16	6353.0
17	6353.0
18	10567.5
19	14782.0
20	14782.0
21	14782.0
22	23046.5
23	31311.0
24	31311.0
25	31311.0
26	46201.0
27	61091.0
28	61091.0
29	61091.0
30	76820.5
31	92550.0
32	92550.0
33	92550.0
34	115872.5
35	139195.0
36	139195.0
37	139195.0
38	165708.0
39	192221.0
40	192221.0
41	192221.0
42	221879.5
43	251538.0
44	251538.0
45	251538.0
46	280626.5
47	309715.0
48	309715.0
49	309715.0
50	320713.0
51	331711.0
52	331711.0
53	331711.0
54	313929.5
55	296148.0
56	296148.0
57	296148.0
58	273043.5
59	249939.0
60	249939.0
61	249939.0
62	218664.0
63	187389.0
64	187389.0
65	187389.0
66	153342.5
67	119296.0
68	119296.0
69	119296.0
70	90841.0
71	62386.0
72	62386.0
73	62386.0
74	47180.5
75	31975.0
76	31975.0
77	31975.0
78	25041.0
79	18107.0
80	18107.0
81	18107.0
82	12629.0
83	7151.0
84	7151.0
85	7151.0
86	5270.5
87	3390.0
88	3390.0
89	3390.0
90	2275.0
91	1160.0
92	1160.0
93	1160.0
94	728.0
95	296.0
96	296.0
97	296.0
98	672.0
99	1048.0
100	1048.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010347819176412933
2	1.241738301169552E-4
3	6.208691505847759E-4
4	0.010678949390058147
5	0.03418919455886833
6	0.06324587080623584
7	0.10703784156081536
8	0.10968688326997708
9	0.11808931244122439
10	0.13311434588537596
11	0.14023364547874806
12	0.13286599822514206
13	0.12789904502046387
14	0.11573000966900224
15	0.1322037377978516
16	0.11808931244122439
17	0.12380130862660432
18	0.11217035987231619
19	0.09702115259804765
20	0.09197141683995814
21	0.0921783732234864
22	0.10202949707943151
23	0.08063020702260958
24	0.09052272215526035
25	0.08812202810633253
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2415968.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.89168847095443
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.19844969427892	42.9244493952996
2	12.780090350466574	14.030414762969237
3	3.8017360786456087	6.2605113743340794
4	1.6500078989527518	3.62286878255714
5	0.8893299581283164	2.4408411504733247
6	0.5463417552609828	1.7993772857076178
7	0.3724437895270879	1.431084792736384
8	0.28055333106159147	1.2320036838497137
9	0.2146990039766447	1.0606671757179167
>10	1.1225082070117705	11.082832584411815
>50	0.07800288945467745	2.951958604699007
>100	0.05661190249329946	6.404271733448505
>500	0.006427352156339156	2.433920089252677
>1k	0.0027977885856999936	2.324798584543072
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3872	0.1602670234042835	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0695638352825866E-4
2	0.0	0.0	0.0	0.0	2.0695638352825866E-4
3	0.0	0.0	0.0	0.0	2.0695638352825866E-4
4	0.0	0.0	0.0	0.0	2.0695638352825866E-4
5	0.0	0.0	0.0	0.0	2.0695638352825866E-4
6	0.0	0.0	0.0	0.0	2.483476602339104E-4
7	0.0	0.0	0.0	4.139127670565173E-5	2.483476602339104E-4
8	0.0	0.0	0.0	4.139127670565173E-5	2.483476602339104E-4
9	0.0	0.0	0.0	4.139127670565173E-5	2.483476602339104E-4
10	0.0	0.0	0.0	1.241738301169552E-4	2.897389369395621E-4
11	0.0	0.0	0.0	1.241738301169552E-4	3.3113021364521383E-4
12	0.0	0.0	0.0	1.241738301169552E-4	7.450429807017311E-4
13	0.0	0.0	0.0	1.241738301169552E-4	9.519993642299898E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACG	35	0.0021682158	16.28847	9
CCGATCA	65	3.3680735E-6	14.617857	9
GGTATCA	830	0.0	13.385228	1
CGATCAA	95	7.380913E-8	13.00166	10
GTATCAA	2335	0.0	12.891112	1
CCGATAA	155	1.8189894E-12	12.260138	9
ATAACGA	160	3.6379788E-12	11.87627	12
CGGAATT	80	2.8671933E-5	11.875286	15
CGCAAGA	515	0.0	11.803662	2
TAACGAA	155	2.5465852E-11	11.645682	13
TTAGAGT	235	0.0	11.318716	4
CGTCGTA	350	0.0	11.129992	10
TCTTATA	215	0.0	11.044487	2
TAGACTG	250	0.0	11.0204935	5
GATAACG	190	0.0	11.001861	11
ACTCTAA	330	0.0	10.94079	10
CGATAAC	165	8.0035534E-11	10.94079	10
CAAGACG	530	0.0	10.933545	4
AAGACGG	550	0.0	10.882305	5
CGCCGGT	405	0.0	10.791501	7
>>END_MODULE