FastQCFastQC Report
Thu 2 Feb 2017
SRR4062190_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062190_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2415968
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27150.027.3140641
GGTATCA12000.025.1226581
TCAACGC38500.018.9700644
CAACGCA39550.018.5220555
ATCAACG39550.018.4664333
AACGCAG40950.017.8351026
TATCAAC41350.017.7165072
CGTCGTA5850.017.67430710
GTATACG902.2124898E-417.1151921
ACCGTCG6400.016.8425378
AACGCGA800.001989530916.49949613
TATACTG3650.016.2728025
ATACCGT6800.016.1753066
CCGTCGT6600.015.9988499
TACCGTC6500.015.9065487
GTAATAC2800.015.7131543
GCGAAAG8300.015.638418
CGTCTTA2750.015.19953415
AAGACGG9600.015.1239115
CGAGCCG7800.014.94826215
Produced by FastQC (version 0.11.5)