##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062190_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2415968
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.294686022331422	32.0	32.0	32.0	32.0	32.0
2	31.459578520907563	32.0	32.0	32.0	32.0	32.0
3	31.581518049907945	32.0	32.0	32.0	32.0	32.0
4	31.657121700287423	32.0	32.0	32.0	32.0	32.0
5	31.630644942317115	32.0	32.0	32.0	32.0	32.0
6	35.28127483476602	36.0	36.0	36.0	36.0	36.0
7	35.28627862620697	36.0	36.0	36.0	36.0	36.0
8	35.23967246254917	36.0	36.0	36.0	36.0	36.0
9	35.3198966211473	36.0	36.0	36.0	36.0	36.0
10	35.202642584669995	36.0	36.0	36.0	36.0	36.0
11	35.323118518126066	36.0	36.0	36.0	36.0	36.0
12	35.24379834501119	36.0	36.0	36.0	36.0	36.0
13	35.278963959787546	36.0	36.0	36.0	36.0	36.0
14	35.2447933913032	36.0	36.0	36.0	36.0	36.0
15	35.2220041821746	36.0	36.0	36.0	36.0	36.0
16	35.23247741691943	36.0	36.0	36.0	36.0	36.0
17	35.21626362600829	36.0	36.0	36.0	36.0	36.0
18	35.21268948926476	36.0	36.0	36.0	36.0	36.0
19	35.20167444270785	36.0	36.0	36.0	36.0	36.0
20	35.20577880170598	36.0	36.0	36.0	36.0	36.0
21	35.20213057457715	36.0	36.0	36.0	36.0	36.0
22	35.182762354468274	36.0	36.0	36.0	36.0	36.0
23	35.150699429793775	36.0	36.0	36.0	36.0	36.0
24	35.133166084981255	36.0	36.0	36.0	36.0	36.0
25	35.118307444469465	36.0	36.0	36.0	36.0	36.0
26	35.07931975920211	36.0	36.0	36.0	36.0	36.0
27	35.065892015126025	36.0	36.0	36.0	36.0	36.0
28	35.04973989721718	36.0	36.0	36.0	36.0	36.0
29	35.03517058172956	36.0	36.0	36.0	36.0	36.0
30	35.00860193512497	36.0	36.0	36.0	36.0	36.0
31	35.01653581504391	36.0	36.0	36.0	36.0	36.0
32	34.99031361342534	36.0	36.0	36.0	36.0	36.0
33	34.96961921681082	36.0	36.0	36.0	36.0	36.0
34	34.95764596219818	36.0	36.0	36.0	36.0	36.0
35	34.94968600577491	36.0	36.0	36.0	36.0	36.0
36	34.9192075391727	36.0	36.0	36.0	36.0	36.0
37	34.91207830567292	36.0	36.0	36.0	36.0	36.0
38	34.90401362931959	36.0	36.0	36.0	32.0	36.0
39	34.88411642869442	36.0	36.0	36.0	32.0	36.0
40	34.87575249341051	36.0	36.0	36.0	32.0	36.0
41	34.85299184426284	36.0	36.0	36.0	32.0	36.0
42	34.820510039868076	36.0	36.0	36.0	32.0	36.0
43	34.81889122703612	36.0	36.0	36.0	32.0	36.0
44	34.777844739665426	36.0	36.0	36.0	32.0	36.0
45	34.76172945999285	36.0	36.0	36.0	32.0	36.0
46	34.744648521834726	36.0	36.0	36.0	32.0	36.0
47	34.72145657558378	36.0	36.0	36.0	32.0	36.0
48	34.7015556497437	36.0	36.0	36.0	32.0	36.0
49	34.68701654988808	36.0	36.0	36.0	32.0	36.0
50	34.284632495132385	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	9.0
20	18.0
21	52.0
22	183.0
23	470.0
24	1154.0
25	2596.0
26	5590.0
27	10198.0
28	17693.0
29	28627.0
30	42693.0
31	63610.0
32	97351.0
33	166430.0
34	384639.0
35	1594655.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.16133314232291	17.583985929267072	12.115346555560432	26.139334372849582
2	16.34508676717306	19.535746055294386	36.93550009458435	27.183667082948197
3	18.693878463320203	23.001189588246834	28.35429530762487	29.950636640808092
4	12.79156544770686	15.538995358794223	35.08106691854649	36.58837227495243
5	14.833079121593235	36.50081851351323	33.058715668480296	15.607386696413233
6	35.53996022293369	34.609607341165955	16.11285759520523	13.737574840695126
7	31.25095199936423	30.029992119100918	19.80146260215367	18.917593279381183
8	28.538623028119574	33.15408151101339	18.57284533569981	19.73445012516722
9	27.1546229089127	14.345637028305012	18.255995112518047	40.243744950264244
10	16.301843651773247	26.70174166358301	30.924234860921057	26.072179823722685
11	37.66241936979298	21.18239976688433	21.624293036993866	19.530887826328826
12	24.6279752049696	23.357552749043034	28.3366749890727	23.677797056914663
13	29.555855044437674	19.28527199035749	25.049007271619487	26.109865693585345
14	23.575105299407937	19.174674498999984	24.829385157419303	32.42083504417277
15	25.275251990092585	26.996094319130055	21.881870951933138	25.84678273884422
16	26.03374320609714	25.91350080071922	23.161626074679432	24.89112991850421
17	24.43335342189963	26.02493079378535	24.712868713492895	24.82884707082213
18	25.083393178295214	25.221847170799244	25.45628765317378	24.238471997731757
19	25.68054586883502	25.04419559058182	25.078467610250975	24.196790930332185
20	25.829877837280286	24.460110647618873	24.801465919523437	24.908545595577404
21	26.59521980423582	24.16679359991523	24.559141511808104	24.678845084040848
22	26.0311808765679	24.086204784169325	24.906248758261697	24.976365581001073
23	24.73261235248149	24.228797732420297	25.40464112107445	25.633948794023766
24	24.97940751594624	24.73287719428957	25.041040096360405	25.246675193403785
25	25.124774313503274	24.371533989359104	25.11715829967524	25.38653339746238
26	24.970208468286867	24.99363605696298	25.364586674834587	24.671568799915562
27	25.428826281946478	24.678812312315422	24.834113218168387	25.05824818756972
28	25.06147743702521	24.541014691255157	25.277750528058174	25.11975734366146
29	24.87772915642224	24.88799427802254	25.272812163175693	24.961464402379523
30	25.015014853425644	24.85764346066398	25.400247770951363	24.727093914959017
31	25.40729286012305	24.549742710120068	24.748008238574275	25.294956191182607
32	25.27163362635962	24.662180900337674	24.622237822328223	25.443947650974486
33	24.890861144580683	24.571112340896516	24.97496853216466	25.563057982358146
34	25.454853258188102	24.572879172696055	25.167387985814237	24.804879583301602
35	25.751325039890894	24.473367784715425	25.170563315279814	24.604743860113867
36	24.876819152634976	24.861049023990464	25.031747214357853	25.230384609016703
37	25.80821144400003	24.612559764992316	24.792698105608586	24.786530685399065
38	25.058610047815204	24.439603504682182	25.24006940489278	25.26171704260984
39	25.583229095352294	24.523106062801073	24.719136934352008	25.17452790749462
40	25.69166950197147	24.65850098883842	24.971377908463943	24.67845160072617
41	24.843746119522837	25.046896860021356	25.285685902795603	24.823671117660208
42	25.803266962549394	24.94004111230049	24.816687653312204	24.44000427183791
43	25.35016718780916	24.30419819806395	25.066829590959028	25.278805023167866
44	25.051472907492816	24.776867947052654	24.845167341795836	25.326491803658698
45	25.198289999693696	24.97444023070434	24.844592463448496	24.982677306153466
46	25.150461027489456	24.78765747702313	24.853305672235862	25.20857582325155
47	25.162007602850405	24.759182362446687	25.12351320564783	24.955296829055072
48	25.758526542013055	25.11474136831979	24.40265776110284	24.724074328564306
49	25.119552165226	25.172327725050508	24.55483297880575	25.153287130917747
50	25.166477919944903	25.258409163156642	24.551685143977323	25.023427772921135
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	108.0
1	106.5
2	105.0
3	242.0
4	379.0
5	392.5
6	406.0
7	318.5
8	231.0
9	337.5
10	444.0
11	762.5
12	1081.0
13	1994.5
14	2908.0
15	3700.5
16	4493.0
17	4366.5
18	4240.0
19	4116.0
20	3992.0
21	4897.5
22	5803.0
23	7095.5
24	8388.0
25	11032.0
26	13676.0
27	17031.5
28	20387.0
29	23807.0
30	27227.0
31	30983.0
32	34739.0
33	40409.0
34	46079.0
35	53646.5
36	61214.0
37	72110.0
38	83006.0
39	92309.0
40	101612.0
41	111472.0
42	121332.0
43	127505.0
44	133678.0
45	145187.0
46	156696.0
47	166803.5
48	176911.0
49	184336.0
50	191761.0
51	189000.0
52	186239.0
53	184562.5
54	182886.0
55	182501.0
56	182116.0
57	175249.0
58	168382.0
59	154640.0
60	140898.0
61	123690.0
62	106482.0
63	91826.0
64	77170.0
65	65131.0
66	53092.0
67	45988.5
68	38885.0
69	34353.5
70	29822.0
71	23529.0
72	17236.0
73	15073.5
74	12911.0
75	9728.0
76	6545.0
77	5704.0
78	4863.0
79	4154.0
80	3445.0
81	2676.5
82	1908.0
83	1533.5
84	1159.0
85	876.0
86	593.0
87	415.5
88	238.0
89	156.5
90	75.0
91	58.0
92	41.0
93	31.5
94	22.0
95	27.0
96	32.0
97	25.5
98	19.0
99	16.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030712327315593584
2	0.005587822355262983
3	2.483476602339104E-4
4	4.1391276705651736E-5
5	1.241738301169552E-4
6	1.241738301169552E-4
7	0.0
8	0.0
9	0.0
10	8.278255341130347E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.208691505847759E-4
17	0.0
18	1.241738301169552E-4
19	1.241738301169552E-4
20	4.139127670565173E-4
21	0.0
22	0.0
23	0.0
24	0.0015728685148147657
25	2.483476602339104E-4
26	5.380865971734725E-4
27	5.380865971734725E-4
28	0.0016970423449317207
29	8.278255341130346E-4
30	0.0011175644710525966
31	6.622604272904278E-4
32	0.0012417383011695518
33	3.725214903508656E-4
34	9.933906409356416E-4
35	1.241738301169552E-4
36	3.311302136452139E-4
37	0.0019453900051656316
38	0.0
39	9.106080875243381E-4
40	8.278255341130347E-5
41	0.0011589557477582485
42	0.0060431263990251526
43	0.005049735758089511
44	0.005546431078557332
45	0.002566259155750407
46	0.0024834766023391036
47	0.0013245208545808556
48	0.004139127670565173
49	0.003187128306335183
50	0.0013245208545808556
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2415968.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.74227846001551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78822228144212	43.3609274732173
2	13.17109796581994	14.683740208697579
3	3.843096295669826	6.426688315856446
4	1.6693909777498857	3.7222262696148674
5	0.8876155971033806	2.4738857889594787
6	0.5377973365138811	1.7986829332206855
7	0.37744428042177414	1.4727722927687499
8	0.2810389813931167	1.2532602527147385
9	0.21152046552962972	1.061156942059026
>10	1.0974984965570183	10.882446485212345
>50	0.0766113291586445	2.94655654053005
>100	0.05007875061878945	5.6508120263906605
>500	0.00619774858979103	2.3562338484119474
>1k	0.0023894934322076715	1.910610622346194
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.139127670565173E-5
2	0.0	0.0	0.0	0.0	4.139127670565173E-5
3	0.0	0.0	0.0	0.0	4.139127670565173E-5
4	0.0	0.0	0.0	0.0	4.139127670565173E-5
5	0.0	0.0	0.0	0.0	4.139127670565173E-5
6	0.0	0.0	0.0	0.0	8.278255341130346E-5
7	0.0	0.0	0.0	0.0	8.278255341130346E-5
8	0.0	0.0	0.0	0.0	8.278255341130346E-5
9	0.0	0.0	0.0	0.0	8.278255341130346E-5
10	0.0	0.0	0.0	4.139127670565173E-5	1.241738301169552E-4
11	0.0	0.0	0.0	4.139127670565173E-5	1.241738301169552E-4
12	0.0	0.0	0.0	4.139127670565173E-5	4.139127670565173E-4
13	0.0	0.0	0.0	4.139127670565173E-5	4.966953204678208E-4
14	0.0	0.0	0.0	4.139127670565173E-5	4.966953204678208E-4
15	0.0	0.0	0.0	1.241738301169552E-4	6.622604272904277E-4
16	0.0	0.0	0.0	2.0695638352825866E-4	6.622604272904277E-4
17	0.0	0.0	0.0	2.483476602339104E-4	6.622604272904277E-4
18	0.0	0.0	0.0	3.7252149035086555E-4	7.036517039960794E-4
19	0.0	0.0	0.0	4.5530404376216904E-4	7.036517039960794E-4
20	0.0	4.139127670565173E-5	0.0	7.036517039960794E-4	7.450429807017311E-4
21	0.0	4.139127670565173E-5	0.0	7.864342574073829E-4	8.692168108186863E-4
22	0.0	4.139127670565173E-5	0.0	8.692168108186863E-4	9.933906409356416E-4
23	0.0	4.139127670565173E-5	0.0	0.0011589557477582485	0.001076173194346945
24	0.0	4.139127670565173E-5	0.0	0.001365912131286507	0.001076173194346945
25	0.0	4.139127670565173E-5	0.0	0.0018212161750486761	0.0011589557477582485
26	0.0	4.139127670565173E-5	0.0	0.002442085325633452	0.0014900859614034622
27	4.139127670565173E-5	4.139127670565173E-5	0.0	0.002855998092689969	0.0014900859614034622
28	4.139127670565173E-5	4.139127670565173E-5	0.0	0.0038079974569199594	0.0014900859614034622
29	4.139127670565173E-5	4.139127670565173E-5	0.0	0.005380865971734725	0.0014900859614034622
30	4.139127670565173E-5	4.139127670565173E-5	0.0	0.007947125127485133	0.0014900859614034622
31	4.139127670565173E-5	4.139127670565173E-5	0.0	0.012251817904872913	0.0014900859614034622
32	4.139127670565173E-5	4.139127670565173E-5	0.0	0.016225380468615477	0.0016142597915204174
33	4.139127670565173E-5	4.139127670565173E-5	0.0	0.020488681969297606	0.0016142597915204174
34	4.139127670565173E-5	4.139127670565173E-5	0.0	0.026407634538205806	0.0018626074517543278
35	4.139127670565173E-5	4.139127670565173E-5	0.0	0.03187128306335183	0.0018626074517543278
36	4.139127670565173E-5	4.139127670565173E-5	0.0	0.04023232095789348	0.0018626074517543278
37	4.139127670565173E-5	8.278255341130346E-5	0.0	0.05244274758606074	0.0018626074517543278
38	4.139127670565173E-5	8.278255341130346E-5	0.0	0.06866812805467622	0.0019039987284599797
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2715	0.0	27.314064	1
GGTATCA	1200	0.0	25.122658	1
TCAACGC	3850	0.0	18.970064	4
CAACGCA	3955	0.0	18.522055	5
ATCAACG	3955	0.0	18.466433	3
AACGCAG	4095	0.0	17.835102	6
TATCAAC	4135	0.0	17.716507	2
CGTCGTA	585	0.0	17.674307	10
GTATACG	90	2.2124898E-4	17.115192	1
ACCGTCG	640	0.0	16.842537	8
AACGCGA	80	0.0019895309	16.499496	13
TATACTG	365	0.0	16.272802	5
ATACCGT	680	0.0	16.175306	6
CCGTCGT	660	0.0	15.998849	9
TACCGTC	650	0.0	15.906548	7
GTAATAC	280	0.0	15.713154	3
GCGAAAG	830	0.0	15.6384	18
CGTCTTA	275	0.0	15.199534	15
AAGACGG	960	0.0	15.123911	5
CGAGCCG	780	0.0	14.948262	15
>>END_MODULE