FastQCFastQC Report
Thu 2 Feb 2017
SRR4062189_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062189_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGAAGCAGTGGTATCAA33.8461538461538463No Hit
GTACATGGAAGCAGTGGTATCAACG22.564102564102564No Hit
CTCCTAGAGACAAATGTCAAATGAC11.282051282051282No Hit
GTATCAACGCAGAGTACTTTTTTTT11.282051282051282No Hit
GAGTAGATCTCGGTGGACCACTAAA11.282051282051282No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11.282051282051282No Hit
GCAGACTACAAATCCCAGCGCAAAA11.282051282051282No Hit
TCCCAGGACCGAGTGGGAGTAGGGG11.282051282051282No Hit
GCTCTACACACTGAGTCCACGACAC11.282051282051282No Hit
GGGCCCTAGCTGTCTTCTGCTCAAC11.282051282051282No Hit
TATCAACGCAGAGTACATTTAATAA11.282051282051282No Hit
ACTGAAAATCATGGAAAATGAGAAA11.282051282051282No Hit
GGAGCACTGTCTCTTTCAAGAGCTG11.282051282051282No Hit
AGTCCACAGAGACGCCCTCGCATCA11.282051282051282No Hit
GAACTGGATCTAGAGAATTTTGAGT11.282051282051282No Hit
GGCCTGCTTTGAACACTCTAATTTT11.282051282051282No Hit
GAGCAGTACCGCTCCGTCCTCACGC11.282051282051282No Hit
ATACATGTAAGCAGTGTTATCAACA11.282051282051282No Hit
TATCAACGCGGAGTACATGGGCGCT11.282051282051282No Hit
GATAGAAACCGACCTGGATTGCTCC11.282051282051282No Hit
CCTTTATTTCCATCTTTCTCAAATT11.282051282051282No Hit
GTGTGCACGTGCGCGCTCTTCTGCA11.282051282051282No Hit
AAGTACATAGAAGCAGTGGTATCAA11.282051282051282No Hit
TATCAACGCAGAGTACATGGGAGGT11.282051282051282No Hit
CCTCAATGTCTGCTTTGCATACACA11.282051282051282No Hit
GGATGGAGCCACCGATCCACACAGA11.282051282051282No Hit
CTACTCAAATTAAGAAACCTCAATA11.282051282051282No Hit
CTTACACGGCAGCCACACAGTGTTC11.282051282051282No Hit
GTCCAAGAATTTCACCTCTAGCGGC11.282051282051282No Hit
CCTCAAAGTGTTCGATGGGATCCAT11.282051282051282No Hit
TATCAACGCAGAGTACATGGAAAGA11.282051282051282No Hit
TAATAATCCTGTCTCAGCCTCTTGA11.282051282051282No Hit
GTGTAGTGCCTGGGGGGGGGGGGGG11.282051282051282No Hit
GTATCAACGCAGAGTACATTTGAAT11.282051282051282No Hit
CTTCTGAGTGGTCCATAATAGCCAA11.282051282051282No Hit
GTAAATGGAAGAAGTGGTATCAACG11.282051282051282No Hit
ACGCAGAGTACATGGAAGCAGTGGT11.282051282051282No Hit
GTAGGGCACTGCATACTTCTCAGCC11.282051282051282No Hit
TCGGAAAGCAGCTCAGTCTCCTGTC11.282051282051282No Hit
GTGCTAGGGCTGTGATCTCCTTCTG11.282051282051282No Hit
TTATTGGGGAATCTAAGCTTCTCTG11.282051282051282No Hit
GTACATGGAAGCACTGGTATCAACG11.282051282051282No Hit
GTACATGGGGTCTCTCGGGTGAGAC11.282051282051282No Hit
ACAGGAACCTGCTGCTCCAGCCGCT11.282051282051282No Hit
ATGGAGAGAGATAAAGGAAACCTGC11.282051282051282No Hit
CACCCAGGCTGACTTGTCTCGGAAC11.282051282051282No Hit
ATGTATTTCTCATCTGCTTCTCGCT11.282051282051282No Hit
CAACATTACGCTAGCCAGCACTACA11.282051282051282No Hit
CTGCAGGTCCCTGAGGAGTACATGG11.282051282051282No Hit
AGAGTGTTCAAAGCAGGCCCGAGCC11.282051282051282No Hit
TTTCTAAATTTTCCACTTTTATCAG11.282051282051282No Hit
GTTAATTTCTCTCTTGAGCCTCTCC11.282051282051282No Hit
GGACGATGAGGATGATGAGGATGAT11.282051282051282No Hit
GCTGGTTCCTTCGAGCATTGAGATC11.282051282051282No Hit
TGCCAGGTAAGACCCCGGCGTTATT11.282051282051282No Hit
CTGTGTCTCTTCTTCTGGCGGGAGG11.282051282051282No Hit
CTGTAGAACCTGGAATATGGCGAGA11.282051282051282No Hit
ATCAGGGGCTGCCTGAGCCCCTCAC11.282051282051282No Hit
GTATCAACGCAGAGTACATTGGAAG11.282051282051282No Hit
GAGTACATGGAAGCAGTGGTATAAA11.282051282051282No Hit
AGCCAGGAGGCTGCCGACGAAATCT11.282051282051282No Hit
CAGTATGGCCGAGCCCAAGACCGTC11.282051282051282No Hit
TATCAACGAAGAGTACATGTGAAGC11.282051282051282No Hit
AATCAACTCGTCTATGTGGCAAAAT11.282051282051282No Hit
CATTGGAAGCAGTGGTATCAACGCA11.282051282051282No Hit
CAGTGGTATCAACGCAGAGTACATG11.282051282051282No Hit
CTGCAGGACGATCATCAGGGTCAAA11.282051282051282No Hit
GTACATGGGTACCTGGTTGATCCTG11.282051282051282No Hit
AAGCAGTGGTATCAACGCAGAGTAC11.282051282051282No Hit
GAGTACATGGGAAGCAGTGGTATCA11.282051282051282No Hit
ATTCAGTCTTGTTTCCATTATTTCC11.282051282051282No Hit
GCTCCGTCTCGCTCTTCTCTCTGTA11.282051282051282No Hit
CGTGAAATATGACGAGGAAAACTGA11.282051282051282No Hit
GTGTGTGTTTCTCATTTTCCAAGTT11.282051282051282No Hit
GCATTTGCTGACCTGTGTGTAGATC11.282051282051282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCAGG50.019.01
CCCAGGA50.019.02
GAGTGGG50.019.011
AGTAGGG50.019.018
AGGACCG50.019.05
GGACCGA50.019.06
AGTGGGA50.019.012
GGGAGTA50.019.015
ACCGAGT50.019.08
GTGGGAG50.019.013
GAGTAGG50.019.017
GACCGAG50.019.07
CCGAGTG50.019.09
CGAGTGG50.019.010
GTAGGGG50.019.019
TGGGAGT50.019.014
CAGGACC50.019.04
GGAGTAG50.019.016
CCAGGAC50.019.03
Produced by FastQC (version 0.11.5)