##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062189_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	78
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.397435897435898	NaN	NaN	NaN	NaN	NaN
2	27.32051282051282	NaN	NaN	NaN	NaN	NaN
3	26.397435897435898	NaN	NaN	NaN	NaN	NaN
4	27.03846153846154	NaN	NaN	NaN	NaN	NaN
5	27.833333333333332	NaN	NaN	NaN	NaN	NaN
6	30.397435897435898	NaN	NaN	NaN	NaN	NaN
7	30.025641025641026	NaN	NaN	NaN	NaN	NaN
8	28.21794871794872	NaN	NaN	NaN	NaN	NaN
9	28.0	NaN	NaN	NaN	NaN	NaN
10	28.628205128205128	NaN	NaN	NaN	NaN	NaN
11	28.743589743589745	NaN	NaN	NaN	NaN	NaN
12	28.55128205128205	NaN	NaN	NaN	NaN	NaN
13	27.423076923076923	NaN	NaN	NaN	NaN	NaN
14	28.397435897435898	NaN	NaN	NaN	NaN	NaN
15	27.102564102564102	NaN	NaN	NaN	NaN	NaN
16	28.897435897435898	NaN	NaN	NaN	NaN	NaN
17	27.384615384615383	NaN	NaN	NaN	NaN	NaN
18	29.21794871794872	NaN	NaN	NaN	NaN	NaN
19	30.05128205128205	NaN	NaN	NaN	NaN	NaN
20	29.55128205128205	NaN	NaN	NaN	NaN	NaN
21	28.692307692307693	NaN	NaN	NaN	NaN	NaN
22	28.58974358974359	NaN	NaN	NaN	NaN	NaN
23	28.807692307692307	NaN	NaN	NaN	NaN	NaN
24	28.397435897435898	NaN	NaN	NaN	NaN	NaN
25	28.692307692307693	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	1.0
19	3.0
20	2.0
21	5.0
22	2.0
23	3.0
24	2.0
25	1.0
26	6.0
27	3.0
28	7.0
29	9.0
30	5.0
31	6.0
32	3.0
33	5.0
34	7.0
35	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.15384615384615	17.94871794871795	14.102564102564102	21.794871794871796
2	12.82051282051282	30.76923076923077	39.743589743589745	16.666666666666664
3	32.05128205128205	29.48717948717949	28.205128205128204	10.256410256410255
4	12.82051282051282	6.41025641025641	32.05128205128205	48.717948717948715
5	15.384615384615385	57.692307692307686	15.384615384615385	11.538461538461538
6	30.76923076923077	35.8974358974359	19.230769230769234	14.102564102564102
7	28.205128205128204	32.05128205128205	23.076923076923077	16.666666666666664
8	34.61538461538461	25.64102564102564	26.923076923076923	12.82051282051282
9	26.923076923076923	16.666666666666664	19.230769230769234	37.17948717948718
10	21.794871794871796	23.076923076923077	28.205128205128204	26.923076923076923
11	38.46153846153847	26.923076923076923	19.230769230769234	15.384615384615385
12	14.102564102564102	38.46153846153847	24.358974358974358	23.076923076923077
13	29.48717948717949	19.230769230769234	21.794871794871796	29.48717948717949
14	28.205128205128204	15.384615384615385	25.64102564102564	30.76923076923077
15	16.666666666666664	35.8974358974359	29.48717948717949	17.94871794871795
16	34.61538461538461	15.384615384615385	23.076923076923077	26.923076923076923
17	29.48717948717949	29.48717948717949	25.64102564102564	15.384615384615385
18	21.794871794871796	15.384615384615385	35.8974358974359	26.923076923076923
19	30.76923076923077	19.230769230769234	26.923076923076923	23.076923076923077
20	23.076923076923077	20.51282051282051	39.743589743589745	16.666666666666664
21	17.94871794871795	33.33333333333333	19.230769230769234	29.48717948717949
22	17.94871794871795	29.48717948717949	29.48717948717949	23.076923076923077
23	24.358974358974358	37.17948717948718	20.51282051282051	17.94871794871795
24	20.51282051282051	30.76923076923077	23.076923076923077	25.64102564102564
25	19.230769230769234	32.05128205128205	21.794871794871796	26.923076923076923
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.0
14	0.5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.5
23	1.0
24	1.0
25	1.0
26	2.5
27	4.0
28	4.0
29	4.0
30	3.5
31	3.0
32	3.0
33	3.0
34	4.0
35	5.0
36	5.0
37	5.0
38	7.5
39	10.0
40	10.0
41	10.0
42	9.0
43	8.0
44	8.0
45	8.0
46	11.5
47	15.0
48	15.0
49	15.0
50	11.0
51	7.0
52	7.0
53	7.0
54	7.5
55	8.0
56	8.0
57	8.0
58	8.5
59	9.0
60	9.0
61	9.0
62	6.0
63	3.0
64	3.0
65	3.0
66	3.0
67	3.0
68	3.0
69	3.0
70	1.5
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.5
79	1.0
80	1.0
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	78.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	96.15384615384616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.33333333333334	93.58974358974359
2	1.3333333333333335	2.564102564102564
3	1.3333333333333335	3.8461538461538463
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTACATGGAAGCAGTGGTATCAA	3	3.8461538461538463	No Hit
GTACATGGAAGCAGTGGTATCAACG	2	2.564102564102564	No Hit
CTCCTAGAGACAAATGTCAAATGAC	1	1.282051282051282	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1	1.282051282051282	No Hit
GAGTAGATCTCGGTGGACCACTAAA	1	1.282051282051282	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1	1.282051282051282	No Hit
GCAGACTACAAATCCCAGCGCAAAA	1	1.282051282051282	No Hit
TCCCAGGACCGAGTGGGAGTAGGGG	1	1.282051282051282	No Hit
GCTCTACACACTGAGTCCACGACAC	1	1.282051282051282	No Hit
GGGCCCTAGCTGTCTTCTGCTCAAC	1	1.282051282051282	No Hit
TATCAACGCAGAGTACATTTAATAA	1	1.282051282051282	No Hit
ACTGAAAATCATGGAAAATGAGAAA	1	1.282051282051282	No Hit
GGAGCACTGTCTCTTTCAAGAGCTG	1	1.282051282051282	No Hit
AGTCCACAGAGACGCCCTCGCATCA	1	1.282051282051282	No Hit
GAACTGGATCTAGAGAATTTTGAGT	1	1.282051282051282	No Hit
GGCCTGCTTTGAACACTCTAATTTT	1	1.282051282051282	No Hit
GAGCAGTACCGCTCCGTCCTCACGC	1	1.282051282051282	No Hit
ATACATGTAAGCAGTGTTATCAACA	1	1.282051282051282	No Hit
TATCAACGCGGAGTACATGGGCGCT	1	1.282051282051282	No Hit
GATAGAAACCGACCTGGATTGCTCC	1	1.282051282051282	No Hit
CCTTTATTTCCATCTTTCTCAAATT	1	1.282051282051282	No Hit
GTGTGCACGTGCGCGCTCTTCTGCA	1	1.282051282051282	No Hit
AAGTACATAGAAGCAGTGGTATCAA	1	1.282051282051282	No Hit
TATCAACGCAGAGTACATGGGAGGT	1	1.282051282051282	No Hit
CCTCAATGTCTGCTTTGCATACACA	1	1.282051282051282	No Hit
GGATGGAGCCACCGATCCACACAGA	1	1.282051282051282	No Hit
CTACTCAAATTAAGAAACCTCAATA	1	1.282051282051282	No Hit
CTTACACGGCAGCCACACAGTGTTC	1	1.282051282051282	No Hit
GTCCAAGAATTTCACCTCTAGCGGC	1	1.282051282051282	No Hit
CCTCAAAGTGTTCGATGGGATCCAT	1	1.282051282051282	No Hit
TATCAACGCAGAGTACATGGAAAGA	1	1.282051282051282	No Hit
TAATAATCCTGTCTCAGCCTCTTGA	1	1.282051282051282	No Hit
GTGTAGTGCCTGGGGGGGGGGGGGG	1	1.282051282051282	No Hit
GTATCAACGCAGAGTACATTTGAAT	1	1.282051282051282	No Hit
CTTCTGAGTGGTCCATAATAGCCAA	1	1.282051282051282	No Hit
GTAAATGGAAGAAGTGGTATCAACG	1	1.282051282051282	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1	1.282051282051282	No Hit
GTAGGGCACTGCATACTTCTCAGCC	1	1.282051282051282	No Hit
TCGGAAAGCAGCTCAGTCTCCTGTC	1	1.282051282051282	No Hit
GTGCTAGGGCTGTGATCTCCTTCTG	1	1.282051282051282	No Hit
TTATTGGGGAATCTAAGCTTCTCTG	1	1.282051282051282	No Hit
GTACATGGAAGCACTGGTATCAACG	1	1.282051282051282	No Hit
GTACATGGGGTCTCTCGGGTGAGAC	1	1.282051282051282	No Hit
ACAGGAACCTGCTGCTCCAGCCGCT	1	1.282051282051282	No Hit
ATGGAGAGAGATAAAGGAAACCTGC	1	1.282051282051282	No Hit
CACCCAGGCTGACTTGTCTCGGAAC	1	1.282051282051282	No Hit
ATGTATTTCTCATCTGCTTCTCGCT	1	1.282051282051282	No Hit
CAACATTACGCTAGCCAGCACTACA	1	1.282051282051282	No Hit
CTGCAGGTCCCTGAGGAGTACATGG	1	1.282051282051282	No Hit
AGAGTGTTCAAAGCAGGCCCGAGCC	1	1.282051282051282	No Hit
TTTCTAAATTTTCCACTTTTATCAG	1	1.282051282051282	No Hit
GTTAATTTCTCTCTTGAGCCTCTCC	1	1.282051282051282	No Hit
GGACGATGAGGATGATGAGGATGAT	1	1.282051282051282	No Hit
GCTGGTTCCTTCGAGCATTGAGATC	1	1.282051282051282	No Hit
TGCCAGGTAAGACCCCGGCGTTATT	1	1.282051282051282	No Hit
CTGTGTCTCTTCTTCTGGCGGGAGG	1	1.282051282051282	No Hit
CTGTAGAACCTGGAATATGGCGAGA	1	1.282051282051282	No Hit
ATCAGGGGCTGCCTGAGCCCCTCAC	1	1.282051282051282	No Hit
GTATCAACGCAGAGTACATTGGAAG	1	1.282051282051282	No Hit
GAGTACATGGAAGCAGTGGTATAAA	1	1.282051282051282	No Hit
AGCCAGGAGGCTGCCGACGAAATCT	1	1.282051282051282	No Hit
CAGTATGGCCGAGCCCAAGACCGTC	1	1.282051282051282	No Hit
TATCAACGAAGAGTACATGTGAAGC	1	1.282051282051282	No Hit
AATCAACTCGTCTATGTGGCAAAAT	1	1.282051282051282	No Hit
CATTGGAAGCAGTGGTATCAACGCA	1	1.282051282051282	No Hit
CAGTGGTATCAACGCAGAGTACATG	1	1.282051282051282	No Hit
CTGCAGGACGATCATCAGGGTCAAA	1	1.282051282051282	No Hit
GTACATGGGTACCTGGTTGATCCTG	1	1.282051282051282	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1	1.282051282051282	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1	1.282051282051282	No Hit
ATTCAGTCTTGTTTCCATTATTTCC	1	1.282051282051282	No Hit
GCTCCGTCTCGCTCTTCTCTCTGTA	1	1.282051282051282	No Hit
CGTGAAATATGACGAGGAAAACTGA	1	1.282051282051282	No Hit
GTGTGTGTTTCTCATTTTCCAAGTT	1	1.282051282051282	No Hit
GCATTTGCTGACCTGTGTGTAGATC	1	1.282051282051282	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCCAGG	5	0.0	19.0	1
CCCAGGA	5	0.0	19.0	2
GAGTGGG	5	0.0	19.0	11
AGTAGGG	5	0.0	19.0	18
AGGACCG	5	0.0	19.0	5
GGACCGA	5	0.0	19.0	6
AGTGGGA	5	0.0	19.0	12
GGGAGTA	5	0.0	19.0	15
ACCGAGT	5	0.0	19.0	8
GTGGGAG	5	0.0	19.0	13
GAGTAGG	5	0.0	19.0	17
GACCGAG	5	0.0	19.0	7
CCGAGTG	5	0.0	19.0	9
CGAGTGG	5	0.0	19.0	10
GTAGGGG	5	0.0	19.0	19
TGGGAGT	5	0.0	19.0	14
CAGGACC	5	0.0	19.0	4
GGAGTAG	5	0.0	19.0	16
CCAGGAC	5	0.0	19.0	3
>>END_MODULE