FastQCFastQC Report
Thu 2 Feb 2017
SRR4062189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22.564102564102564No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA22.564102564102564No Hit
GTCTAGGTAGCGGCTTCACCGCCAACGGCACGGCCATGGCTGGAGCGCTG11.282051282051282No Hit
GAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
GTACATGGGAAGTAGTGGTATAAACGCAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
AGGATGCAGCCAGCTGGGCCGTCCAGAGGCTCCACCTGGCCGCCACTGCA11.282051282051282No Hit
GCAGACAGCCACGCAGATCAAGTTTCCATCCACAGGCGTGGACACGATGC11.282051282051282No Hit
GGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
TATCAACGCAGAGTACATGGGTTATCAAAGAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
CCTTTGATCTCGGTGTTGATGGTTTTAAGTCTTTTCCATTTTGGTTTGAT11.282051282051282No Hit
GCCAAGCACACGAGGGTAGCCTTTTGTTTGTTTGCAATCTCTGCTTTTGA11.282051282051282No Hit
GGCGGGCGGTCCGCCGCGAGGCGAGTCACCGCCCGGCCCCGCCCCTTGCC11.282051282051282No Hit
CCCCACGAGGCCTCCGGACCTTCAAGACTGTATTTGCTGAGCACATCAGC11.282051282051282No Hit
GGACTATAGCGAGAAAACTAAAAAAAAAAAAAAAAAAAATAAAAAAAACC11.282051282051282No Hit
GTACATGGGATTTTCGTGACGCCGCGAGACCGAGAATCTGTAGGAGCAGA11.282051282051282No Hit
GGCACTGACTGGCCGTGATAGTGTCGGTCCCCGCGGATGCTAGATTTTCG11.282051282051282No Hit
TGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
CTTCTGTACCCTGAAGATGCCATTGGCCACTGTGGCTGGCCAGCTGCGGG11.282051282051282No Hit
TTTGTGGATGTGGTCTTGGTGGACCAGCACCACTGGCGGTACCAGAGCGG11.282051282051282No Hit
GAGGCTACACAGCTCCACGGGCAGGGACTGCAGCTCATTGCTGCTCACAT11.282051282051282No Hit
GTTTCTCATTTTCCATGATTTTCAGTTTTCTTGCCATATTCCACGTCCTA11.282051282051282No Hit
GAGTCCTGCAGGGAGATGGCCCCGCAGGCTGGAGGCAAAGACGAGTGGGA11.282051282051282No Hit
CCGCCGAGCCGCGTCCGTGTGCCGCCACCCACTCCGGACACAGAATATCC11.282051282051282No Hit
GCTCTCAGACCCTGTCCCACTATCTGAAATTTTCTTATTTTCTAAATCAA11.282051282051282No Hit
GGGTGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGACCCCTGATT11.282051282051282No Hit
AGAGGAGGCTGTGGCCACCGGGCCCCTGAACTTGTCCACCCGGCGTTTGA11.282051282051282No Hit
ACAATCCCCGTGTGGAAGCCTTTGTAGCACCAATTCCGAAGCAGATCTAC11.282051282051282No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAT11.282051282051282No Hit
CCTCTGCACCCCCGCACCTGCCTCACCATATGGCTCGGACACCACTCCCT11.282051282051282No Hit
GAGTACATGGGTGGTATCAAAGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
ATATTTATTATTCTGATCAAAATACTTATTGAAGATTTATTGCTCAGCTA11.282051282051282No Hit
AGTCCACTCCAGGCAGCTGTCTTCACGCTAGAGAATGAGGGTCATGAACC11.282051282051282No Hit
GTCCTGGAACTTGCTTTGTAGACCAGGCTGACCTCAAACTCACAGAGATC11.282051282051282No Hit
ACATGGGAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
GTTTGAGGCCATCAGCCTGATCATCCACCATGACAGTGAGCCAAACTTGC11.282051282051282No Hit
CAGCTGAGCCCCTATGTCCCATGGCCGAGGCACCCAGCCCTTCAGTGAAG11.282051282051282No Hit
CACACACAGTGCCCATCTATGAGGGCTACGCCCTTCCCCACGCCATCTTG11.282051282051282No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC11.282051282051282No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
GCTTCTTCTTCCGATAGTGCATCTTGGCCTTTTCCTTCAGTTTCTCCTCC11.282051282051282No Hit
ATGGGGTGGTATCAACGCAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAT11.282051282051282No Hit
GTGGTATCAACGCAGAGTACATGGGATTTTCGTGACGCCGCGAGACCGAG11.282051282051282No Hit
ATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCCTACAA11.282051282051282No Hit
CCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACA11.282051282051282No Hit
TCAGGGAGCTGTGGATGTAGACACGCTGGAGCCTGTGGATGCGGCTTCCA11.282051282051282No Hit
GCTCCAGGCAGGCTGTCTGCCTCTCTTCCAGCTTCTCCTGGTGACGCTCC11.282051282051282No Hit
CCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGG11.282051282051282No Hit
CTCTCCAGCTGGCGCCTCTTCCGCTCCTCAGCGTACATGTCTCGGGCCTT11.282051282051282No Hit
ACAGTGTGCATTCCTCATTTTTCACGTGTTTCAGTGATTTCGTCATTTTT11.282051282051282No Hit
GCAGAGTACATGGGAAGTAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
CTTCCAATAGTCAGCTAAGGAAATCTCAGGATCTCCTTCAAAAGAGTCTG11.282051282051282No Hit
CTCAGACCACCACGCAGCTGGTCAGAGTAGCCCAGGGGGCCGCCAGACAA11.282051282051282No Hit
GTCCTGACTCCATCCTCGTCCCCCAGGGCTTCAGGGGCCCGGAGCTTTGA11.282051282051282No Hit
GGACAAAGCAAGTTCAAACAACGCTGAATTGAGACAATTACAAATCAATT11.282051282051282No Hit
GTCATGAAGTGTGATGTGGATATCCGCAAAGACCTGTATGCCAATACAGT11.282051282051282No Hit
TGTTGAAGCCATCCAGAGGTTTGGTTCCATTAGTGCTTCCCCCAGCCAGA11.282051282051282No Hit
ATCGTGCGTGACATCAAAGAGAAGCTGTGCTATGTTGCTCTAGACTTCGA11.282051282051282No Hit
GGGTGGTATCAACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
ACGCCGCGAGACCGAGAATCTGTAGGAGCAGAACCAGAGGGAAGCGGGTG11.282051282051282No Hit
CCCCACGACCGGGCGGACCCGCGCGTGCAGGAGCTGAAGGACAGGCTGGA11.282051282051282No Hit
AGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAAATCCGCCGAT11.282051282051282No Hit
GCCTGCCTCCTCACCCGAGTACAGGGTCCATCCACTAAAAGCCCTGTTCT11.282051282051282No Hit
CCCTGTCCGACTACAACATCCAGAAAGAGTCCACCTTGCACCTGGTGCTG11.282051282051282No Hit
GCCCAGGGGCAGGGTGCCCTTCCAGCAGCAGAGGAGAGGTGTAATACCCC11.282051282051282No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTAACTA11.282051282051282No Hit
GGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAAG11.282051282051282No Hit
AGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAACTTAAAAAA11.282051282051282No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG11.282051282051282No Hit
CACCTCCACTGTCATCGCAAAAAAAAAAAAAAAAAAAAAAAGATAAATTT11.282051282051282No Hit
TATCAACGCAGAGTACATGGGGTATAAAAGAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
GCACTGTCCGGTTGTTCATGGAGTTGGCGGCCAGGACGATGGCAAGGTAC11.282051282051282No Hit
GGGTGGAAGAGCTGGCGGTAGGTGCCGGTGCGAACTTCATCGATGACCGT11.282051282051282No Hit
GGGCTACAGGCTGGCTGGCTTCACTCAAAGGCCACGCACTTAATCTTGAA11.282051282051282No Hit
GGGTGTGTCTGCAGATCGGGTACCCAACTGTTGCCTCGGTGCCACCCTCC11.282051282051282No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTTTAAAAAAAAAAAAAAAAT11.282051282051282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTGCA50.044.02
TGCACCC50.044.05
GCCTCAC50.044.020
ACCACTC50.044.041
CCTGCCT50.044.017
ACCATAT50.044.025
ATGGCTC50.044.030
ACACCAC50.044.039
ACTCCCT50.044.044
GGACACC50.044.037
CGGACAC50.044.036
TCGGACA50.044.035
CCGCACC50.044.012
GACACCA50.044.038
ACCTGCC50.044.016
TGCCTCA50.044.019
CCCGCAC50.044.011
CCACTCC50.044.042
TCTGCAC50.044.03
CCTCTGC50.044.01