FastQCFastQC Report
Thu 2 Feb 2017
SRR4062188_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062188_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177680
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC31811.7902971634398919No Hit
CCCATGTACTCTGCGTTGATACCAC23751.3366726699684826No Hit
GTATCAACGCAGAGTACATGGGAAG16080.9049977487618189No Hit
GTACATGGAAGCAGTGGTATCAACG14960.8419630796938317No Hit
GTATCAACGCAGAGTACTTTTTTTT14040.790184601530842No Hit
TCCATGTACTCTGCGTTGATACCAC11800.6641152633948673No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10590.5960153084196308No Hit
CATGTACTCTGCGTTGATACCACTG9670.5442368302566412No Hit
TATCAACGCAGAGTACATGGGAAGC9330.525101305718145No Hit
GAGTACATGGGAAGCAGTGGTATCA9300.5234128770823954No Hit
TATCAACGCAGAGTACTTTTTTTTT9280.5222872579918956No Hit
GCGTTGATACCACTGCTTCCCATGT8650.48683025664115265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA7310.41141377757766767No Hit
GGTATCAACGCAGAGTACATGGGAA7110.40015758667266993No Hit
ACGCAGAGTACATGGGAAGCAGTGG6730.37877082395317424No Hit
GAGTACATGGAAGCAGTGGTATCAA6150.34612787032868075No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5950.334871679423683No Hit
ACTCTGCGTTGATACCACTGCTTCC5760.32417829806393517No Hit
GCTTCCCATGTACTCTGCGTTGATA5650.3179873930661864No Hit
GGTATCAACGCAGAGTACTTTTTTT5470.3078568212516884No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5420.305042773525439No Hit
CAGTGGTATCAACGCAGAGTACATG5080.2859072489869428No Hit
AAAAAGTACTCTGCGTTGATACCAC4640.2611436289959478No Hit
GTCCTAAAGTGTGTATTTCTCATTT4210.23694281855020263No Hit
GCTTCCATGTACTCTGCGTTGATAC3930.22118415128320576No Hit
GTCCTACAGTGGACATTTCTAAATT3880.2183701035569563No Hit
CTGTAGGACGTGGAATATGGCAAGA3760.21161638901395768No Hit
CTTTAGGACGTGAAATATGGCGAGG3630.20429986492570915No Hit
GTACTCTGCGTTGATACCACTGCTT3620.20373705538045928No Hit
GCGTTGATACCACTGCTTCCATGTA3330.18741557856821253No Hit
GTACATGGGAGTGGTATCAACGCAA3280.1846015308419631No Hit
CATGGAAGCAGTGGTATCAACGCAG3220.18122467357046376No Hit
GTGGTATCAACGCAGAGTACATGGG3080.17334533993696533No Hit
GTATCAACGCAGAGTACATGGGAGT2710.1525213867627195No Hit
GTATCAACGCAGAGTACATGGAAGC2630.1480189104007204No Hit
ATCAACGCAGAGTACATGGGAAGCA2510.14126519585772174No Hit
CTGCTTCCCATGTACTCTGCGTTGA2450.1378883385862224No Hit
GGGAAGCAGTGGTATCAACGCAGAG2390.1345114813147231No Hit
GCAGAGTACTTTTTTTTTTTTTTTT2350.13226024313372353No Hit
ATACCACTGCTTCCCATGTACTCTG2310.130009004952724No Hit
AAGCAGTGGTATCAACGCAGAGTAC2200.12381809995497524No Hit
AAAGTACTCTGCGTTGATACCACTG2160.1215668617739757No Hit
CATGGGAAGCAGTGGTATCAACGCA2150.1210040522287258No Hit
GCAGTGGTATCAACGCAGAGTACAT2080.11706438541197658No Hit
GTTGATACCACTGCTTCCCATGTAC2070.1165015758667267No Hit
ACGCAGAGTACATGGAAGCAGTGGT1970.11087348041422783No Hit
TACCACTGCTTCCCATGTACTCTGC1930.10862224223322828No Hit
CTGAAGGACCTGGAATATGGCGAGA1840.10355695632597928No Hit
GTCCTTCAGTGTGCATTTCTCATTT1780.10018009905447996No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCCAC759.555333E-713.93064810
CACCTTT500.00149260813.29743714
TCCAGGG500.00149260813.2974372
AAATGTC701.0767019E-412.2188157
TTCCACC550.00305448212.08857911
CTTTAGG1501.2732926E-1112.0276261
GAAATGT855.24145E-511.1806156
ATGTCCA855.24145E-511.1806159
AATGTCC855.24145E-511.1806158
TTAGGAC1451.1714292E-911.1420623
TTTAGGA1451.1823431E-911.1357842
ATGGGAT600.005849372611.08119718
AGGACCT1502.05182E-910.7706595
TGGCGAG2050.010.65648318
AAAAAGT909.451103E-510.5505491
TAGGACC1002.3560255E-510.4538764
GTCCTAA1201.5039695E-610.2867851
TTTAGAA750.002632074310.131382
GGCGAGA1054.057431E-59.95046219
GGCGAGG1151.0121472E-59.91113319
Produced by FastQC (version 0.11.5)