##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062188_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	177680
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.919270598829357	32.0	32.0	32.0	32.0	32.0
2	30.733757316524088	32.0	32.0	32.0	32.0	32.0
3	30.780245384961727	32.0	32.0	32.0	32.0	32.0
4	30.706477937865827	32.0	32.0	32.0	32.0	32.0
5	30.801795362449347	32.0	32.0	32.0	32.0	32.0
6	34.321718820351194	36.0	36.0	36.0	32.0	36.0
7	34.19806956325979	36.0	36.0	36.0	32.0	36.0
8	34.12216906798739	36.0	36.0	36.0	32.0	36.0
9	34.07107158937416	36.0	36.0	36.0	32.0	36.0
10	34.01556168392616	36.0	36.0	36.0	32.0	36.0
11	34.24356145880234	36.0	36.0	36.0	32.0	36.0
12	34.093730301665914	36.0	36.0	36.0	32.0	36.0
13	34.06067649707339	36.0	36.0	36.0	32.0	36.0
14	34.02617627194957	36.0	36.0	36.0	32.0	36.0
15	33.92236605132823	36.0	36.0	36.0	32.0	36.0
16	33.97767897343539	36.0	36.0	36.0	32.0	36.0
17	33.871386762719496	36.0	36.0	36.0	32.0	36.0
18	33.81376632147681	36.0	36.0	36.0	32.0	36.0
19	33.7961728950923	36.0	36.0	36.0	32.0	36.0
20	33.70994484466456	36.0	36.0	36.0	27.0	36.0
21	33.64166479063485	36.0	36.0	36.0	27.0	36.0
22	33.48867627194957	36.0	36.0	36.0	21.0	36.0
23	33.49714655560558	36.0	36.0	36.0	21.0	36.0
24	33.452211841512835	36.0	36.0	36.0	21.0	36.0
25	32.93709477712742	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	4.0
5	17.0
6	43.0
7	16.0
8	22.0
9	28.0
10	8.0
11	2.0
12	8.0
13	7.0
14	150.0
15	191.0
16	212.0
17	274.0
18	332.0
19	481.0
20	551.0
21	685.0
22	928.0
23	1185.0
24	1458.0
25	1870.0
26	2239.0
27	2823.0
28	3558.0
29	4683.0
30	6133.0
31	8252.0
32	11530.0
33	15777.0
34	33919.0
35	80293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.21112349306048	17.867152939587346	12.088722295388287	23.833001271963887
2	12.991332733003153	22.09027465105808	39.89813147230977	25.020261143628996
3	19.230639351643404	29.19011706438541	27.487055380459253	24.09218820351193
4	11.941911516379601	18.382303275920297	35.16492176066644	34.51086344703366
5	11.716880528357553	39.40328928476918	33.975012246138945	14.904817940734318
6	31.01908173380193	34.61126881139047	19.499076326935207	14.870573127872397
7	26.44543354555186	32.70832159558285	22.380979210096346	18.465265648768945
8	27.346849080418316	34.28540840245222	20.813424089433827	17.554318427695637
9	28.326523711572598	15.752486686015384	19.310208797092056	36.61078080531996
10	15.978809141375717	29.655366754022598	32.211232282244204	22.15459182235748
11	31.81800249110922	23.972135647096618	22.8714260754885	21.338435786305663
12	25.63891597022243	26.14892166199866	29.977627374625946	18.234534993152963
13	30.52318373419291	22.229673376462365	24.327141987692446	22.920000901652276
14	22.777013929585717	23.089753414777082	26.714150475589417	27.41908218004778
15	26.338805204758444	27.866533673705153	25.242176801744687	20.55248431979172
16	20.677433169544244	27.23876391831583	30.759477978632287	21.32432493350764
17	22.255345047167154	27.864686059485837	28.92975080866027	20.950218084686735
18	23.43272817426778	24.58613656084835	32.073430474323	19.90770479056087
19	23.140109410299893	28.08272815878578	29.47430097410097	19.302861456813357
20	24.0838492696145	25.812212338529317	30.25480398176994	19.84913441008625
21	22.293517511788135	26.963703248849352	27.871262865544843	22.871516373817666
22	22.915445981881284	28.948821381890294	25.53296975706495	22.602762879163475
23	19.179116248676177	30.240091034047634	27.971563126704073	22.609229590572117
24	21.6243317390839	30.605082444666028	27.831764434154117	19.93882138209596
25	22.35704347140306	26.910358891160946	26.90472569133445	23.827871946101546
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	846.0
1	846.0
2	635.0
3	424.0
4	424.0
5	424.0
6	944.5
7	1465.0
8	1465.0
9	1465.0
10	1314.5
11	1164.0
12	1164.0
13	1164.0
14	1015.0
15	866.0
16	866.0
17	866.0
18	1318.5
19	1771.0
20	1771.0
21	1771.0
22	2832.0
23	3893.0
24	3893.0
25	3893.0
26	5688.0
27	7483.0
28	7483.0
29	7483.0
30	9446.5
31	11410.0
32	11410.0
33	11410.0
34	12203.0
35	12996.0
36	12996.0
37	12996.0
38	14133.5
39	15271.0
40	15271.0
41	15271.0
42	17539.0
43	19807.0
44	19807.0
45	19807.0
46	28150.5
47	36494.0
48	36494.0
49	36494.0
50	30613.5
51	24733.0
52	24733.0
53	24733.0
54	19615.0
55	14497.0
56	14497.0
57	14497.0
58	12153.0
59	9809.0
60	9809.0
61	9809.0
62	8334.5
63	6860.0
64	6860.0
65	6860.0
66	5462.0
67	4064.0
68	4064.0
69	4064.0
70	3127.5
71	2191.0
72	2191.0
73	2191.0
74	1533.0
75	875.0
76	875.0
77	875.0
78	644.0
79	413.0
80	413.0
81	413.0
82	295.0
83	177.0
84	177.0
85	177.0
86	119.5
87	62.0
88	62.0
89	62.0
90	44.0
91	26.0
92	26.0
93	26.0
94	18.5
95	11.0
96	11.0
97	11.0
98	41.5
99	72.0
100	72.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011256190904997748
2	0.0
3	0.0
4	0.011256190904997748
5	0.04108509680324178
6	0.07203962179198559
7	0.10693381359747861
8	0.11706438541197658
9	0.13226024313372353
10	0.1378883385862224
11	0.14013957676722197
12	0.130009004952724
13	0.12832057631697433
14	0.1215668617739757
15	0.12775776677172446
16	0.1215668617739757
17	0.12719495722647456
18	0.1159387663214768
19	0.10299414678072939
20	0.09624043223773075
21	0.09624043223773075
22	0.10355695632597928
23	0.09230076542098155
24	0.0939891940567312
25	0.09061233678523188
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	177680.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.03266059943475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.24051351664968	48.30668469034391
2	11.578932329132117	13.439124999206934
3	2.558556931244331	4.454395980457007
4	0.8970069233016366	2.082227933412283
5	0.4479977504059549	1.299925069930953
6	0.23599863253215583	0.821737712620159
7	0.19899883759176587	0.8083902401151489
8	0.11599932208533051	0.5385399430674
9	0.09999941557354794	0.5222908929709382
>10	0.48399717137063	5.304734502882689
>50	0.0699995909007037	2.8540295684471757
>100	0.049999707786216936	6.226868090573038
>500	0.014999912335865083	6.201333869139187
>1k	0.006999959090070371	7.139716506833185
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3181	1.7902971634398919	No Hit
CCCATGTACTCTGCGTTGATACCAC	2375	1.3366726699684826	No Hit
GTATCAACGCAGAGTACATGGGAAG	1608	0.9049977487618189	No Hit
GTACATGGAAGCAGTGGTATCAACG	1496	0.8419630796938317	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1404	0.790184601530842	No Hit
TCCATGTACTCTGCGTTGATACCAC	1180	0.6641152633948673	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1059	0.5960153084196308	No Hit
CATGTACTCTGCGTTGATACCACTG	967	0.5442368302566412	No Hit
TATCAACGCAGAGTACATGGGAAGC	933	0.525101305718145	No Hit
GAGTACATGGGAAGCAGTGGTATCA	930	0.5234128770823954	No Hit
TATCAACGCAGAGTACTTTTTTTTT	928	0.5222872579918956	No Hit
GCGTTGATACCACTGCTTCCCATGT	865	0.48683025664115265	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	731	0.41141377757766767	No Hit
GGTATCAACGCAGAGTACATGGGAA	711	0.40015758667266993	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	673	0.37877082395317424	No Hit
GAGTACATGGAAGCAGTGGTATCAA	615	0.34612787032868075	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	595	0.334871679423683	No Hit
ACTCTGCGTTGATACCACTGCTTCC	576	0.32417829806393517	No Hit
GCTTCCCATGTACTCTGCGTTGATA	565	0.3179873930661864	No Hit
GGTATCAACGCAGAGTACTTTTTTT	547	0.3078568212516884	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	542	0.305042773525439	No Hit
CAGTGGTATCAACGCAGAGTACATG	508	0.2859072489869428	No Hit
AAAAAGTACTCTGCGTTGATACCAC	464	0.2611436289959478	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	421	0.23694281855020263	No Hit
GCTTCCATGTACTCTGCGTTGATAC	393	0.22118415128320576	No Hit
GTCCTACAGTGGACATTTCTAAATT	388	0.2183701035569563	No Hit
CTGTAGGACGTGGAATATGGCAAGA	376	0.21161638901395768	No Hit
CTTTAGGACGTGAAATATGGCGAGG	363	0.20429986492570915	No Hit
GTACTCTGCGTTGATACCACTGCTT	362	0.20373705538045928	No Hit
GCGTTGATACCACTGCTTCCATGTA	333	0.18741557856821253	No Hit
GTACATGGGAGTGGTATCAACGCAA	328	0.1846015308419631	No Hit
CATGGAAGCAGTGGTATCAACGCAG	322	0.18122467357046376	No Hit
GTGGTATCAACGCAGAGTACATGGG	308	0.17334533993696533	No Hit
GTATCAACGCAGAGTACATGGGAGT	271	0.1525213867627195	No Hit
GTATCAACGCAGAGTACATGGAAGC	263	0.1480189104007204	No Hit
ATCAACGCAGAGTACATGGGAAGCA	251	0.14126519585772174	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	245	0.1378883385862224	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	239	0.1345114813147231	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	235	0.13226024313372353	No Hit
ATACCACTGCTTCCCATGTACTCTG	231	0.130009004952724	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	220	0.12381809995497524	No Hit
AAAGTACTCTGCGTTGATACCACTG	216	0.1215668617739757	No Hit
CATGGGAAGCAGTGGTATCAACGCA	215	0.1210040522287258	No Hit
GCAGTGGTATCAACGCAGAGTACAT	208	0.11706438541197658	No Hit
GTTGATACCACTGCTTCCCATGTAC	207	0.1165015758667267	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	197	0.11087348041422783	No Hit
TACCACTGCTTCCCATGTACTCTGC	193	0.10862224223322828	No Hit
CTGAAGGACCTGGAATATGGCGAGA	184	0.10355695632597928	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	178	0.10018009905447996	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCCAC	75	9.555333E-7	13.930648	10
CACCTTT	50	0.001492608	13.297437	14
TCCAGGG	50	0.001492608	13.297437	2
AAATGTC	70	1.0767019E-4	12.218815	7
TTCCACC	55	0.003054482	12.088579	11
CTTTAGG	150	1.2732926E-11	12.027626	1
GAAATGT	85	5.24145E-5	11.180615	6
ATGTCCA	85	5.24145E-5	11.180615	9
AATGTCC	85	5.24145E-5	11.180615	8
TTAGGAC	145	1.1714292E-9	11.142062	3
TTTAGGA	145	1.1823431E-9	11.135784	2
ATGGGAT	60	0.0058493726	11.081197	18
AGGACCT	150	2.05182E-9	10.770659	5
TGGCGAG	205	0.0	10.656483	18
AAAAAGT	90	9.451103E-5	10.550549	1
TAGGACC	100	2.3560255E-5	10.453876	4
GTCCTAA	120	1.5039695E-6	10.286785	1
TTTAGAA	75	0.0026320743	10.13138	2
GGCGAGA	105	4.057431E-5	9.950462	19
GGCGAGG	115	1.0121472E-5	9.911133	19
>>END_MODULE