FastQCFastQC Report
Thu 2 Feb 2017
SRR4062188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177680
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11290.6354119765871229No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9500.5346690679873931No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8280.4660063034669068No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG6480.36470058532192706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.3084196307969383No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG5030.2830932012606934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4700.26452048626744706No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.23469158036920307No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA4030.22681224673570463No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC3750.2110535794687078No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3480.19585772174696084No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.16940567312021612No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2960.16659162539396669No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2950.16602881584871682No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2730.15364700585321925No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC2430.13676271949572266No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.13282305267897343No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.11256190904997748No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1950.10974786132372805No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA1920.1080594326879784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGATG207.838747E-443.99971841
CTGCGAT301.2967197E-436.66642840
TAACACT250.002347484735.1997764
CTATGCT300.005732180629.3331434
GCGATGA300.005732180629.33314342
ATTGAAT300.005732180629.3331433
AGTAACA300.005732180629.3331432
AAAAGTA1650.026.6664942
ATACATG801.2932833E-724.756811
AGTACTC1850.022.594455
AAAAAGT1900.022.0060541
TAGAACA500.002571957721.999864
AAAGTAC1950.021.435763
CTTTAGG2300.021.0492691
TAGGACC956.799128E-720.8419724
TTTAGGA2200.019.9998722
GGATGCA550.004468997519.99987238
AACGCCT550.004468997519.99987236
CGTTTAT550.004468997519.99987244
AAGTACT2100.019.9046364
Produced by FastQC (version 0.11.5)