FastQCFastQC Report
Thu 2 Feb 2017
SRR4062187_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062187_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636017
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32970.20152602326259447No Hit
TATCAACGCAGAGTACTTTTTTTTT20180.12334835151468476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGT402.760717E-416.62544
CGTCGTA1900.013.50156610
CGGTTCT2650.012.905972512
CTCGACG751.4783254E-512.66697213
GGTATCA8150.012.5846391
CCGTCGT2050.012.5147959
GTATCAA22900.012.3582061
TCGAACG550.003070116512.0900923
CGACCAT2050.012.05017810
CCGTCCA951.0372623E-612.0024949
CGGTCCA1900.012.00139210
CGATAAC802.8631706E-511.87637810
TCCAACG2400.011.8745618
CGAACGA802.8676588E-511.87455916
GATATAC5350.011.7156061
GAACGTC658.020118E-411.6929485
GCCGGTC2150.011.4904088
ACTGTTC6050.011.4648428
CGTCTTA1251.8326318E-811.39957815
TGTAGAA7250.011.3974852
Produced by FastQC (version 0.11.5)