##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062187_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1636017
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.253224141313936	32.0	32.0	32.0	32.0	32.0
2	30.801959270594377	32.0	32.0	32.0	32.0	32.0
3	30.812381533932715	32.0	32.0	32.0	32.0	32.0
4	30.819174250634315	32.0	32.0	32.0	32.0	32.0
5	30.730067597097097	32.0	32.0	32.0	32.0	32.0
6	34.40781544446054	36.0	36.0	36.0	32.0	36.0
7	34.32928203068794	36.0	36.0	36.0	32.0	36.0
8	34.27784124492594	36.0	36.0	36.0	32.0	36.0
9	34.41141809651122	36.0	36.0	36.0	32.0	36.0
10	34.078098821711514	36.0	36.0	36.0	32.0	36.0
11	34.42851571835745	36.0	36.0	36.0	32.0	36.0
12	34.212633487304835	36.0	36.0	36.0	32.0	36.0
13	34.31203404365603	36.0	36.0	36.0	32.0	36.0
14	34.209872513549676	36.0	36.0	36.0	32.0	36.0
15	34.15829053121086	36.0	36.0	36.0	32.0	36.0
16	34.165023957575016	36.0	36.0	36.0	32.0	36.0
17	34.099541752928	36.0	36.0	36.0	32.0	36.0
18	34.10572017283439	36.0	36.0	36.0	32.0	36.0
19	34.099632216535646	36.0	36.0	36.0	32.0	36.0
20	34.07616546771825	36.0	36.0	36.0	32.0	36.0
21	34.056244525576446	36.0	36.0	36.0	32.0	36.0
22	34.01667892204054	36.0	36.0	36.0	32.0	36.0
23	33.98651908873808	36.0	36.0	36.0	32.0	36.0
24	33.95585926063116	36.0	36.0	36.0	32.0	36.0
25	33.59509039331498	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	20.0
5	115.0
6	374.0
7	70.0
8	282.0
9	248.0
10	112.0
11	23.0
12	75.0
13	77.0
14	201.0
15	372.0
16	541.0
17	783.0
18	1043.0
19	1449.0
20	2205.0
21	3238.0
22	4938.0
23	7160.0
24	9993.0
25	14166.0
26	19115.0
27	24676.0
28	32731.0
29	42906.0
30	56542.0
31	77132.0
32	108875.0
33	154957.0
34	339283.0
35	732313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.08756412740579	17.659952530591365	11.949259199719316	25.303224142283533
2	16.064266110842844	19.76522148397626	39.022133116545746	25.14837928863515
3	19.443816220722905	23.549424635361973	28.46908874417331	28.53767039974181
4	12.533331051152235	15.974391482900147	36.47019125085123	35.02208621509638
5	14.254512680631045	37.056352177722665	33.99205232575559	14.697082815890703
6	33.538659708689764	36.136952753526394	17.0231284228708	13.301259114913044
7	29.68609645955968	30.746637621920627	20.410093865113264	19.157172053406434
8	27.07638024533615	34.405195065862344	19.36327154034535	19.155153148456154
9	26.752348960019486	14.895769393416607	18.868974144151146	39.482907502412765
10	15.79008254548081	27.470641450489236	31.846910757109647	24.892365246920306
11	36.66621777135721	21.647241742366738	22.587718224333393	19.098822261942654
12	24.20132185716157	23.913336214209906	29.623347831569102	22.261994097059425
13	29.229680750403634	20.359949911071343	25.252862180777946	25.157507157747077
14	22.81563231108448	20.472597068559192	25.626760550451415	31.085010069904907
15	24.539135672043567	28.375274976159552	22.57032473200153	24.515264619795346
16	24.792764806644747	26.735833803035842	24.62048955546865	23.850911834850763
17	22.992008303823994	26.91600579211308	25.877905389774202	24.21408051428872
18	23.6572674829529	26.243934916583495	27.104443964412056	22.99435363605155
19	25.05258907101819	25.486456555125216	25.73474794569161	23.726206428164982
20	24.99452417865739	25.65914817021912	25.64599396252458	23.700333688598914
21	25.77234752656068	25.03369604121226	25.233761506058556	23.9601949261685
22	24.85394322693074	25.53295119121628	25.859819763372116	23.75328581848086
23	23.92135678991425	25.489669483422173	25.911907516289688	24.67706621037389
24	24.10108540904583	25.77413629497479	25.9879632841623	24.136815011817074
25	24.224331702279436	25.67655699572843	25.947644760621735	24.151466541370404
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	721.0
1	721.0
2	672.5
3	624.0
4	624.0
5	624.0
6	1265.5
7	1907.0
8	1907.0
9	1907.0
10	2328.5
11	2750.0
12	2750.0
13	2750.0
14	3981.5
15	5213.0
16	5213.0
17	5213.0
18	8922.5
19	12632.0
20	12632.0
21	12632.0
22	20275.5
23	27919.0
24	27919.0
25	27919.0
26	41795.0
27	55671.0
28	55671.0
29	55671.0
30	72675.0
31	89679.0
32	89679.0
33	89679.0
34	104083.0
35	118487.0
36	118487.0
37	118487.0
38	132172.5
39	145858.0
40	145858.0
41	145858.0
42	162719.5
43	179581.0
44	179581.0
45	179581.0
46	195257.0
47	210933.0
48	210933.0
49	210933.0
50	212262.0
51	213591.0
52	213591.0
53	213591.0
54	197033.0
55	180475.0
56	180475.0
57	180475.0
58	163011.0
59	145547.0
60	145547.0
61	145547.0
62	126432.5
63	107318.0
64	107318.0
65	107318.0
66	87574.5
67	67831.0
68	67831.0
69	67831.0
70	51177.5
71	34524.0
72	34524.0
73	34524.0
74	26079.5
75	17635.0
76	17635.0
77	17635.0
78	13795.0
79	9955.0
80	9955.0
81	9955.0
82	6921.0
83	3887.0
84	3887.0
85	3887.0
86	2819.5
87	1752.0
88	1752.0
89	1752.0
90	1196.5
91	641.0
92	641.0
93	641.0
94	402.5
95	164.0
96	164.0
97	164.0
98	443.0
99	722.0
100	722.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.779849475891754E-4
2	4.278684145702642E-4
3	6.723646514675581E-4
4	0.009474229179770137
5	0.03484071375786438
6	0.06473037871855855
7	0.10739497205713633
8	0.11314063362422273
9	0.12200362221174964
10	0.1375902573139521
11	0.14529188877621688
12	0.13783475355084943
13	0.1297663777332387
14	0.1185806748951875
15	0.13685676860326024
16	0.12249261468554422
17	0.12658792665357388
18	0.11417974263103624
19	0.10067132554246075
20	0.09529240833072028
21	0.09486453991615001
22	0.10519450592506069
23	0.08520693855870691
24	0.09278632190252302
25	0.09113597230346629
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1636017.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.51040432026907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.25898321385509	32.43011890980715
2	16.61110844488132	15.78400956848295
3	6.5364756233358285	9.316517990828041
4	3.086260250232024	5.865178893043931
5	1.6811145930697762	3.9935217012724835
6	0.990685996309291	2.82407353434498
7	0.6343594162446153	2.1097070645106077
8	0.4269135520689482	1.6226268374878376
9	0.2961045278635282	1.2661241255872742
>10	1.308257778179638	10.850128553927524
>50	0.08490456382587727	2.7918400198515942
>100	0.07448696026086786	7.055038603060917
>500	0.007888123403327497	2.5374860879608914
>1k	0.0024569564698888927	1.5536281098337859
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3297	0.20152602326259447	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2018	0.12334835151468476	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.112405922432346E-5	0.0
10	0.0	0.0	0.0	6.112405922432346E-5	0.0
11	0.0	0.0	0.0	6.112405922432346E-5	0.0
12	0.0	0.0	0.0	6.112405922432346E-5	0.0
13	0.0	0.0	0.0	1.2224811844864693E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGT	40	2.760717E-4	16.6254	4
CGTCGTA	190	0.0	13.501566	10
CGGTTCT	265	0.0	12.9059725	12
CTCGACG	75	1.4783254E-5	12.666972	13
GGTATCA	815	0.0	12.584639	1
CCGTCGT	205	0.0	12.514795	9
GTATCAA	2290	0.0	12.358206	1
TCGAACG	55	0.0030701165	12.090092	3
CGACCAT	205	0.0	12.050178	10
CCGTCCA	95	1.0372623E-6	12.002494	9
CGGTCCA	190	0.0	12.001392	10
CGATAAC	80	2.8631706E-5	11.876378	10
TCCAACG	240	0.0	11.87456	18
CGAACGA	80	2.8676588E-5	11.874559	16
GATATAC	535	0.0	11.715606	1
GAACGTC	65	8.020118E-4	11.692948	5
GCCGGTC	215	0.0	11.490408	8
ACTGTTC	605	0.0	11.464842	8
CGTCTTA	125	1.8326318E-8	11.399578	15
TGTAGAA	725	0.0	11.397485	2
>>END_MODULE