FastQCFastQC Report
Thu 2 Feb 2017
SRR4062187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636017
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24300.024.8163241
GCGCTAT551.5943374E-423.99914711
GGTATCA10600.022.4238951
TAGACGT904.0850136E-721.9992224
TAGGACG6850.021.8386424
GATATAC7150.021.5469191
CGCAATA3300.020.66656736
CTAGCGG3400.019.41166929
CTATCGG708.126934E-418.85647614
AGGACGT7650.018.6921485
GTCCTAC6400.018.5697881
AACGCAG33000.018.3326846
GCGCAAT3850.018.28506735
TCAACGC33550.018.0321484
TACGAAT3800.017.94782841
ATCAACG34100.017.9348493
TCTAGCG3700.017.83775128
TAGCGGC3850.017.71420130
CCAACGA5850.017.67512729
TATCAAC34750.017.5993772
Produced by FastQC (version 0.11.5)