FastQCFastQC Report
Thu 2 Feb 2017
SRR4062186_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062186_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952866
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16620.17442116729949436No Hit
TATCAACGCAGAGTACTTTTTTTTT13070.13716514179328468No Hit
GCGCAAGACGGACCAGAGCGAAAGC9960.10452676451883057No Hit
GGTATCAACGCAGAGTACTTTTTTT9730.10211299385223106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTAC508.753597E-515.1907541
GCGCAAG2000.014.7160431
GGTATCA5300.014.6891731
CGGTCCA1300.014.61801410
CCGATAA400.005270817314.2525639
CGCGTAA400.005270817314.25256310
CGATAAC400.005270817314.25256310
TCGCGTA400.005270817314.2525639
TAAACCA551.9576529E-413.8192155
CGCAAGA2150.013.6950982
AAGACGG2100.013.5724425
CAAGACG2100.013.571734
CGCCTCG500.001496178713.30309111
CGCGGTC500.001496771613.30239210
CGCCGGT1403.6379788E-1212.893827
CGTCGTA1051.9846993E-812.66894510
CGGACAT1501.8189894E-1212.6676135
GCAAGAC2400.012.6656153
CCGTCGT1155.298716E-912.3935339
CGTTCAT701.0914235E-412.2126317
Produced by FastQC (version 0.11.5)