FastQCFastQC Report
Thu 2 Feb 2017
SRR4062186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952866
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9720.10200804730150935No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG300.005745984629.3316885
GTATCAA12250.024.9710371
GGTATCA6250.023.943451
ATAATAC808.989895E-519.248923
CGCAATA1504.656613E-1019.06659936
CGAATGC1759.094947E-1218.86103243
GTACGGG600.007397682418.3390431
AACGAGT600.007402163518.33711644
CTATGCG600.00741337618.3323069
GTCCTAA1456.2773324E-918.2125661
TTAAGAC1408.371899E-817.2847443
TAGGGCG800.001988679416.49907531
CCTAGAC1753.623427E-916.341943
TAGCGGC1753.623427E-916.3419430
TCTAGCG1753.623427E-916.3419428
CCAGTAC1906.166374E-1016.2096183
GGCGTTA3300.016.00246242
AAGACGG4300.015.8595765
AGAGCGA4050.015.75220415
TCCGACC3500.015.7134058
Produced by FastQC (version 0.11.5)