##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062186_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	952866
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.273148585425442	32.0	32.0	32.0	32.0	32.0
2	31.45174032865062	32.0	32.0	32.0	32.0	32.0
3	31.57900376338331	32.0	32.0	32.0	32.0	32.0
4	31.655689257461177	32.0	32.0	32.0	32.0	32.0
5	31.623913540833655	32.0	32.0	32.0	32.0	32.0
6	35.27672411440853	36.0	36.0	36.0	36.0	36.0
7	35.284101856924266	36.0	36.0	36.0	36.0	36.0
8	35.22714316598557	36.0	36.0	36.0	36.0	36.0
9	35.32408124542171	36.0	36.0	36.0	36.0	36.0
10	35.19787462245478	36.0	36.0	36.0	36.0	36.0
11	35.32018667892442	36.0	36.0	36.0	36.0	36.0
12	35.23839448568844	36.0	36.0	36.0	36.0	36.0
13	35.276572991375495	36.0	36.0	36.0	36.0	36.0
14	35.240823998337646	36.0	36.0	36.0	36.0	36.0
15	35.21569244783632	36.0	36.0	36.0	36.0	36.0
16	35.2282681930093	36.0	36.0	36.0	36.0	36.0
17	35.20549059364066	36.0	36.0	36.0	36.0	36.0
18	35.208900307073606	36.0	36.0	36.0	36.0	36.0
19	35.19809605967681	36.0	36.0	36.0	36.0	36.0
20	35.19610207521309	36.0	36.0	36.0	36.0	36.0
21	35.19689861953307	36.0	36.0	36.0	36.0	36.0
22	35.17220889401028	36.0	36.0	36.0	36.0	36.0
23	35.149568774623084	36.0	36.0	36.0	36.0	36.0
24	35.1321938236856	36.0	36.0	36.0	36.0	36.0
25	35.11806591902744	36.0	36.0	36.0	36.0	36.0
26	35.08850877248217	36.0	36.0	36.0	36.0	36.0
27	35.06952918878415	36.0	36.0	36.0	36.0	36.0
28	35.04332298560343	36.0	36.0	36.0	36.0	36.0
29	35.03101485413479	36.0	36.0	36.0	36.0	36.0
30	35.015507951800146	36.0	36.0	36.0	36.0	36.0
31	35.01190618617938	36.0	36.0	36.0	36.0	36.0
32	34.99128313949706	36.0	36.0	36.0	36.0	36.0
33	34.96229795165322	36.0	36.0	36.0	36.0	36.0
34	34.95461796307141	36.0	36.0	36.0	36.0	36.0
35	34.941171161527436	36.0	36.0	36.0	36.0	36.0
36	34.92088709220394	36.0	36.0	36.0	36.0	36.0
37	34.911619262309706	36.0	36.0	36.0	36.0	36.0
38	34.899174700325126	36.0	36.0	36.0	36.0	36.0
39	34.88605113415738	36.0	36.0	36.0	32.0	36.0
40	34.870023696931156	36.0	36.0	36.0	32.0	36.0
41	34.84094510665718	36.0	36.0	36.0	32.0	36.0
42	34.81579466577672	36.0	36.0	36.0	32.0	36.0
43	34.797521372365054	36.0	36.0	36.0	32.0	36.0
44	34.760187686411314	36.0	36.0	36.0	32.0	36.0
45	34.74541121206969	36.0	36.0	36.0	32.0	36.0
46	34.73166216445964	36.0	36.0	36.0	32.0	36.0
47	34.69864597960259	36.0	36.0	36.0	32.0	36.0
48	34.67171564522189	36.0	36.0	36.0	32.0	36.0
49	34.65641863598869	36.0	36.0	36.0	32.0	36.0
50	34.254325372088	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	2.0
20	4.0
21	27.0
22	64.0
23	185.0
24	441.0
25	1058.0
26	2189.0
27	4102.0
28	7060.0
29	10947.0
30	17031.0
31	25251.0
32	38696.0
33	66662.0
34	153726.0
35	625419.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.52392310341063	18.328343229555973	12.442260370037541	25.705473296995862
2	16.408787652032824	19.45757773889973	37.297670010715365	26.83596459835208
3	18.998953679595072	22.451915962735463	28.39736667285853	30.151763684810934
4	12.968979898537675	15.726870304953689	34.51786505132936	36.78628474517928
5	14.963069308801028	36.27404063110658	32.847850589694666	15.915039470397726
6	35.66609260722685	34.11550243844078	16.10882151998766	14.109583434344705
7	31.530981271238556	29.760532960563186	19.287391931289395	19.421093836908863
8	28.50211887085907	33.46336210967754	18.317580856070002	19.716938163393383
9	26.518734008769336	14.995917579176925	19.035310316455828	39.45003809559791
10	17.44687594452094	26.513437384558554	29.90384776841186	26.135838902508645
11	37.143417857285286	21.603982091920585	21.26689377100243	19.9857062797917
12	24.407629194451268	23.764831571280748	27.905917516208994	23.92162171805899
13	29.597341074190915	19.15389991877137	25.109721618779556	26.139037388258163
14	23.620110277835497	19.32874087227375	24.84976901264186	32.201379837248886
15	25.36421700427972	26.850050269397798	21.836228808667745	25.949503917654738
16	25.9861112714473	25.94203339633671	23.198395565345976	24.87345976687002
17	24.221978746224547	26.292784085065474	24.73233382238426	24.752903346325716
18	24.983523356953352	25.084272257111195	25.57038570037277	24.36181868556268
19	25.655548629083206	25.08379982075129	25.1674422216765	24.093209328489003
20	25.80197614562399	24.43578051455348	24.93186338043689	24.830379959385642
21	26.53321663276893	24.23362781335466	24.715752267370227	24.51740328650618
22	25.982876920784243	24.083659192373325	25.1481320563437	24.785331830498727
23	24.63221481299574	24.221768853123105	25.65754261354692	25.48847372033423
24	24.756916276350903	24.788295459673552	25.313975490653934	25.140812773321606
25	25.039459985895153	24.29328844410115	25.31987775800114	25.347373812002555
26	24.779427892572176	25.207716114488655	25.490023224744796	24.522832768194377
27	25.204882548697977	24.759697878918587	25.155452567682868	24.87996700470057
28	25.039198030338333	24.621610446091427	25.43621583159645	24.902975691973797
29	24.731021425932767	24.967046713906708	25.519906303522855	24.782025556637674
30	24.737972774565804	24.921210798239823	25.475437604094626	24.865378823099746
31	25.110928736944622	24.777302951942776	24.99160425832018	25.120164052792425
32	25.236733430234082	24.672855021435357	24.796482187927985	25.293929360402572
33	24.839694143772576	24.618571761401917	25.143199568459785	25.398534526365722
34	25.28897275354775	24.662436585514317	25.383425442196007	24.665165218741933
35	25.729799845518354	24.490378480034927	25.298258723175604	24.481562951271115
36	24.618544323790516	24.92341501349092	25.22492740299497	25.233113259723588
37	25.539774844125684	24.784882421280976	25.06540896740414	24.609933767189204
38	24.902557127654884	24.37425619132176	25.36715550769993	25.35603117332343
39	25.41813636645086	24.645094394868497	24.896128388355464	25.04064085032518
40	25.498995135722268	24.69122068708579	25.08057279887497	24.729211378316972
41	24.537127408816044	25.247729452780803	25.470218942251382	24.74492419615177
42	25.657737308094358	24.876575861764966	25.132659958102966	24.333026872037717
43	25.305196740649937	24.30533107759218	25.18250933012044	25.206962851637442
44	24.87447839265174	24.85842086261471	25.126466167938677	25.140634576794874
45	24.95287791075979	25.03862122236169	25.026132294478426	24.982368572400098
46	25.02749663113794	24.842891386208027	25.01259387685707	25.117018105796962
47	25.087342775133674	24.755812794181697	25.302695583273426	24.8541488474112
48	25.568203285377844	25.371525605880603	24.368301519266854	24.6919695894747
49	24.966074852571225	25.33413515274975	24.62740060430317	25.07238939037586
50	24.981424187596815	25.46051223065072	24.771213157972067	24.7868504237804
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	153.0
1	96.5
2	40.0
3	85.5
4	131.0
5	142.5
6	154.0
7	125.5
8	97.0
9	133.5
10	170.0
11	302.5
12	435.0
13	870.0
14	1305.0
15	1540.5
16	1776.0
17	1878.5
18	1981.0
19	1873.0
20	1765.0
21	2033.5
22	2302.0
23	2823.5
24	3345.0
25	4293.0
26	5241.0
27	6529.5
28	7818.0
29	9304.0
30	10790.0
31	12218.0
32	13646.0
33	15969.5
34	18293.0
35	20976.5
36	23660.0
37	27858.0
38	32056.0
39	36118.5
40	40181.0
41	43896.5
42	47612.0
43	50848.5
44	54085.0
45	58812.5
46	63540.0
47	67721.0
48	71902.0
49	74209.5
50	76517.0
51	75480.0
52	74443.0
53	73610.0
54	72777.0
55	72417.5
56	72058.0
57	68908.5
58	65759.0
59	60457.5
60	55156.0
61	47990.5
62	40825.0
63	35195.0
64	29565.0
65	24863.0
66	20161.0
67	17302.0
68	14443.0
69	12718.5
70	10994.0
71	8609.5
72	6225.0
73	5447.5
74	4670.0
75	3486.5
76	2303.0
77	1998.0
78	1693.0
79	1454.5
80	1216.0
81	946.0
82	676.0
83	553.0
84	430.0
85	347.0
86	264.0
87	188.5
88	113.0
89	76.5
90	40.0
91	32.5
92	25.0
93	19.0
94	13.0
95	12.5
96	12.0
97	8.5
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.032953216926619275
2	0.0030434499709298055
3	3.1483965216515226E-4
4	0.0
5	0.0
6	3.1483965216515226E-4
7	0.0
8	0.0
9	0.0
10	2.0989310144343487E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.34625855052022E-4
17	0.0
18	2.0989310144343487E-4
19	0.0
20	1.0494655072171744E-4
21	0.0
22	0.0
23	0.0
24	5.247327536085872E-4
25	2.0989310144343487E-4
26	3.1483965216515226E-4
27	3.1483965216515226E-4
28	0.001259358608660609
29	2.0989310144343487E-4
30	7.34625855052022E-4
31	2.0989310144343487E-4
32	1.0494655072171744E-4
33	0.0
34	8.395724057737395E-4
35	2.0989310144343487E-4
36	3.1483965216515226E-4
37	0.0015741982608257616
38	0.0
39	7.34625855052022E-4
40	1.0494655072171744E-4
41	0.001259358608660609
42	0.005667113738972741
43	0.003987968927425263
44	0.004407755130312132
45	0.002308824115877783
46	0.002308824115877783
47	0.0011544120579388916
48	0.003987968927425263
49	0.003463236173816675
50	0.001469251710104044
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	952866.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.07102633235388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.97645850254882	49.642208616801156
2	12.640712408699867	15.69243985560244
3	3.4062867814486775	6.3429514952054955
4	1.3500347946024327	3.3519218114144635
5	0.7265024114305008	2.2547375155210605
6	0.40487021241327426	1.5078425769534034
7	0.28281897523664906	1.228840484144237
8	0.19589201323426522	0.9727374649409515
9	0.14399062687815967	0.8043881393309754
>10	0.7610772583286972	8.583727686501788
>50	0.062494347330872155	2.6899970880848696
>100	0.0454566737843309	5.493447769497611
>500	0.0034049940634380106	1.4347594960015606
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	972	0.10200804730150935	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.0494655072171742E-4
13	0.0	0.0	0.0	0.0	1.0494655072171742E-4
14	0.0	0.0	0.0	0.0	2.0989310144343485E-4
15	0.0	0.0	0.0	0.0	2.0989310144343485E-4
16	0.0	0.0	0.0	0.0	2.0989310144343485E-4
17	0.0	0.0	0.0	1.0494655072171742E-4	2.0989310144343485E-4
18	0.0	0.0	0.0	2.0989310144343485E-4	3.148396521651523E-4
19	0.0	0.0	0.0	3.148396521651523E-4	3.148396521651523E-4
20	0.0	0.0	0.0	5.247327536085872E-4	4.197862028868697E-4
21	0.0	0.0	0.0	6.296793043303046E-4	4.197862028868697E-4
22	0.0	0.0	0.0	7.34625855052022E-4	5.247327536085872E-4
23	0.0	0.0	0.0	0.0015741982608257613	5.247327536085872E-4
24	0.0	0.0	0.0	0.0026236637680429357	5.247327536085872E-4
25	0.0	0.0	0.0	0.0031483965216515227	5.247327536085872E-4
26	0.0	0.0	0.0	0.003883022376703545	6.296793043303046E-4
27	0.0	0.0	0.0	0.004512701681033849	7.34625855052022E-4
28	0.0	0.0	0.0	0.005457220637529307	7.34625855052022E-4
29	0.0	0.0	0.0	0.006821525796911633	7.34625855052022E-4
30	0.0	0.0	0.0	0.010389708521450025	7.34625855052022E-4
31	0.0	0.0	0.0	0.014797463651762157	8.395724057737394E-4
32	0.0	0.0	0.0	0.02256350840516925	8.395724057737394E-4
33	0.0	0.0	0.0	0.027495996289089965	8.395724057737394E-4
34	0.0	0.0	0.0	0.034002682433836445	9.445189564954568E-4
35	0.0	0.0	0.0	0.039879689274252623	9.445189564954568E-4
36	0.0	0.0	0.0	0.048380359882711735	9.445189564954568E-4
37	0.0	0.0	0.0	0.06107889252003954	0.0010494655072171744
38	0.0	0.0	0.0	0.07629614237468857	0.0010494655072171744
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGCCG	30	0.0057459846	29.331688	5
GTATCAA	1225	0.0	24.971037	1
GGTATCA	625	0.0	23.94345	1
ATAATAC	80	8.989895E-5	19.24892	3
CGCAATA	150	4.656613E-10	19.066599	36
CGAATGC	175	9.094947E-12	18.861032	43
GTACGGG	60	0.0073976824	18.339043	1
AACGAGT	60	0.0074021635	18.337116	44
CTATGCG	60	0.007413376	18.332306	9
GTCCTAA	145	6.2773324E-9	18.212566	1
TTAAGAC	140	8.371899E-8	17.284744	3
TAGGGCG	80	0.0019886794	16.499075	31
CCTAGAC	175	3.623427E-9	16.34194	3
TAGCGGC	175	3.623427E-9	16.34194	30
TCTAGCG	175	3.623427E-9	16.34194	28
CCAGTAC	190	6.166374E-10	16.209618	3
GGCGTTA	330	0.0	16.002462	42
AAGACGG	430	0.0	15.859576	5
AGAGCGA	405	0.0	15.752204	15
TCCGACC	350	0.0	15.713405	8
>>END_MODULE