FastQCFastQC Report
Thu 2 Feb 2017
SRR4062185_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062185_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493855
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33770.22605942343801774No Hit
TATCAACGCAGAGTACTTTTTTTTT21530.14412376033818544No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTCG500.001499418413.30009516
GTAATAC1051.9967047E-812.6650583
AACCGCT752.0716383E-411.4012267
GGTATCA6400.011.2767941
GTCCTAC1950.011.2007041
TCTTATA1451.2223609E-911.1346512
GTATCAA14950.010.988971
CGTCTAT700.001496264110.8528561
TAATACT1909.094947E-1210.5000734
ACCGGCC1002.3953493E-510.4511258
AGAACCG1002.397122E-510.4504245
GTATAAG2001.8189894E-1210.4458731
GTCTTAG2300.010.3220081
TAAGGTT1201.5222777E-610.2920855
GTGTAAT2053.6379788E-1210.1910951
TAGGGCC750.002647311210.1334044
GTAGACC3200.010.0924673
GTAATTC1054.1096413E-59.9511173
CTTATAC1151.0276006E-59.9117853
TAAGGTG1804.1745807E-99.5003865
Produced by FastQC (version 0.11.5)