##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062185_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1493855
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.199317202807503	32.0	32.0	32.0	32.0	32.0
2	30.706861777080103	32.0	32.0	32.0	32.0	32.0
3	30.71602665586687	32.0	32.0	32.0	32.0	32.0
4	30.702750266926845	32.0	32.0	32.0	32.0	32.0
5	30.6148648965261	32.0	32.0	32.0	32.0	32.0
6	34.28080570068715	36.0	36.0	36.0	32.0	36.0
7	34.1648566962657	36.0	36.0	36.0	32.0	36.0
8	34.13329473074696	36.0	36.0	36.0	32.0	36.0
9	34.224122153756554	36.0	36.0	36.0	32.0	36.0
10	33.88073407392284	36.0	36.0	36.0	32.0	36.0
11	34.3052498401786	36.0	36.0	36.0	32.0	36.0
12	34.05703699488906	36.0	36.0	36.0	32.0	36.0
13	34.141416670292635	36.0	36.0	36.0	32.0	36.0
14	34.03873401367603	36.0	36.0	36.0	32.0	36.0
15	33.978559498746534	36.0	36.0	36.0	32.0	36.0
16	33.986518102493214	36.0	36.0	36.0	32.0	36.0
17	33.964623072520425	36.0	36.0	36.0	32.0	36.0
18	33.957006536779005	36.0	36.0	36.0	32.0	36.0
19	33.937136469068285	36.0	36.0	36.0	32.0	36.0
20	33.95067191929605	36.0	36.0	36.0	32.0	36.0
21	33.93946132656784	36.0	36.0	36.0	32.0	36.0
22	33.87671828925833	36.0	36.0	36.0	32.0	36.0
23	33.841436417858496	36.0	36.0	36.0	32.0	36.0
24	33.84338573690218	36.0	36.0	36.0	32.0	36.0
25	33.441915714711264	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	16.0
5	138.0
6	339.0
7	82.0
8	273.0
9	212.0
10	106.0
11	41.0
12	58.0
13	68.0
14	218.0
15	303.0
16	481.0
17	696.0
18	1017.0
19	1404.0
20	2199.0
21	3234.0
22	4901.0
23	7121.0
24	10212.0
25	14631.0
26	19713.0
27	25183.0
28	32912.0
29	43551.0
30	56221.0
31	75529.0
32	106378.0
33	148651.0
34	314643.0
35	623323.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28246185978324	17.053747749074528	11.556937536399724	26.106852854742506
2	16.37003958226088	19.202718343395695	38.37042650170599	26.056815572637433
3	19.45080014941299	22.8350847409974	28.292876240255023	29.421238869334594
4	12.81645474282937	15.715904899052486	35.879565936063344	35.5880744220548
5	14.42405435412953	36.5271830916121	34.41115690027174	14.637605653986627
6	34.35488754441152	35.32906053899371	16.751984116351476	13.564067800243294
7	30.14401651264249	30.39465483618038	20.37535434495607	19.085974306221058
8	27.35621830932113	34.39387786011937	19.00616422657654	19.243739603982956
9	26.690464445421497	14.957346301916333	18.538943282109575	39.81324597055259
10	15.350928350312982	27.823829959525025	32.73532530805357	24.089916382108427
11	36.42963157619531	21.573822187481984	22.27979239877027	19.71675383755244
12	23.862956123033815	23.816769842839282	29.49788340813187	22.82239062599503
13	29.593996755727748	20.50802354108294	24.723566554502433	25.174413148686874
14	22.594393239932014	19.71997029602106	26.135949320937012	31.54968714310991
15	24.724926296201456	27.568907521340247	22.609603816107178	25.096562366351115
16	24.71885110150583	26.6918364105166	24.144857617578516	24.444454870399053
17	23.54980374493276	26.553350813975936	25.613431139137337	24.28341430195397
18	23.77890549184239	26.354061111021753	26.143687035220065	23.72334636191579
19	24.761729898232215	25.784768538094067	25.8295980953134	23.623903468360314
20	25.011173360296812	25.4735193036132	25.895527238541693	23.619780097548293
21	25.11091202821765	25.2368166958032	25.539416737749086	24.112854538230057
22	24.70834134700762	25.454735528644502	25.722319652120536	24.11460347222734
23	24.313117236388976	25.565679981776036	25.584707947525693	24.5364948343093
24	24.06890096039743	25.593489158089337	25.907077377893234	24.43053250362
25	24.34929143354886	25.59228114844718	25.758718885054776	24.299708532949175
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	218.0
1	218.0
2	380.0
3	542.0
4	542.0
5	542.0
6	1309.5
7	2077.0
8	2077.0
9	2077.0
10	2587.5
11	3098.0
12	3098.0
13	3098.0
14	4083.5
15	5069.0
16	5069.0
17	5069.0
18	8732.0
19	12395.0
20	12395.0
21	12395.0
22	18806.0
23	25217.0
24	25217.0
25	25217.0
26	36026.5
27	46836.0
28	46836.0
29	46836.0
30	59365.5
31	71895.0
32	71895.0
33	71895.0
34	85971.0
35	100047.0
36	100047.0
37	100047.0
38	116033.0
39	132019.0
40	132019.0
41	132019.0
42	147545.0
43	163071.0
44	163071.0
45	163071.0
46	176873.5
47	190676.0
48	190676.0
49	190676.0
50	194236.5
51	197797.0
52	197797.0
53	197797.0
54	187380.5
55	176964.0
56	176964.0
57	176964.0
58	158688.5
59	140413.0
60	140413.0
61	140413.0
62	120820.5
63	101228.0
64	101228.0
65	101228.0
66	81480.5
67	61733.0
68	61733.0
69	61733.0
70	46812.0
71	31891.0
72	31891.0
73	31891.0
74	23820.5
75	15750.0
76	15750.0
77	15750.0
78	11991.5
79	8233.0
80	8233.0
81	8233.0
82	5995.5
83	3758.0
84	3758.0
85	3758.0
86	2680.0
87	1602.0
88	1602.0
89	1602.0
90	1051.0
91	500.0
92	500.0
93	500.0
94	348.5
95	197.0
96	197.0
97	197.0
98	413.0
99	629.0
100	629.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0016735225306338298
2	2.677636049014128E-4
3	6.024681110281788E-4
4	0.010576662393605805
5	0.03594726395801467
6	0.06687396032412785
7	0.11145660054021307
8	0.11359870937942439
9	0.12310431735342453
10	0.13850072463525576
11	0.14385599673328403
12	0.13856766553648112
13	0.1335470979445796
14	0.12042668130441041
15	0.1387684882401572
16	0.12397454906935411
17	0.1284595894514528
18	0.11721351804559343
19	0.10282122428214252
20	0.09813536119636777
21	0.09746595218411426
22	0.10804261457772006
23	0.08802728511133946
24	0.09719818857921285
25	0.09405196622162125
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1493855.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.56106378740719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.42581964269833	33.07999040297868
2	16.642823623285295	16.8295773363899
3	7.26055717306776	11.013044830787875
4	3.756042010784534	7.59637918781832
5	2.1779557560772163	5.50598799545854
6	1.3346181322593	4.048782751019562
7	0.8402652682181486	2.9739294077304437
8	0.583740351164947	2.3611626504427488
9	0.4108505380732443	1.8695736236350686
>10	1.5242972048331722	12.08801308057938
>50	0.032525303489386745	1.119178997165916
>100	0.009574226132224365	0.8270915773523578
>500	3.9890139273239625E-4	0.14810679971503546
>1k	5.31868523643195E-4	0.5391813589262753
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3377	0.22605942343801774	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2153	0.14412376033818544	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	6.69409012253532E-5
6	0.0	0.0	0.0	0.0	6.69409012253532E-5
7	0.0	0.0	0.0	0.0	6.69409012253532E-5
8	0.0	0.0	0.0	0.0	6.69409012253532E-5
9	0.0	0.0	0.0	0.0	6.69409012253532E-5
10	0.0	0.0	0.0	0.0	6.69409012253532E-5
11	0.0	0.0	0.0	0.0	6.69409012253532E-5
12	0.0	0.0	0.0	0.0	4.685863085774724E-4
13	6.69409012253532E-5	0.0	0.0	0.0	8.032908147042383E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTCG	50	0.0014994184	13.300095	16
GTAATAC	105	1.9967047E-8	12.665058	3
AACCGCT	75	2.0716383E-4	11.401226	7
GGTATCA	640	0.0	11.276794	1
GTCCTAC	195	0.0	11.200704	1
TCTTATA	145	1.2223609E-9	11.134651	2
GTATCAA	1495	0.0	10.98897	1
CGTCTAT	70	0.0014962641	10.852856	1
TAATACT	190	9.094947E-12	10.500073	4
ACCGGCC	100	2.3953493E-5	10.451125	8
AGAACCG	100	2.397122E-5	10.450424	5
GTATAAG	200	1.8189894E-12	10.445873	1
GTCTTAG	230	0.0	10.322008	1
TAAGGTT	120	1.5222777E-6	10.292085	5
GTGTAAT	205	3.6379788E-12	10.191095	1
TAGGGCC	75	0.0026473112	10.133404	4
GTAGACC	320	0.0	10.092467	3
GTAATTC	105	4.1096413E-5	9.951117	3
CTTATAC	115	1.0276006E-5	9.911785	3
TAAGGTG	180	4.1745807E-9	9.500386	5
>>END_MODULE