FastQCFastQC Report
Thu 2 Feb 2017
SRR4062185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493855
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG551.5934685E-424.00087724
GTATCAA17450.020.8058381
GGTATCA9300.020.3475551
ATACCGT1101.2665441E-719.9993926
TAGGACG1950.019.1789044
TCGTTAC800.001988160716.50060323
GGTCTAT1653.1053787E-816.0027281
AACGCAG23050.015.9387786
TCAACGC22900.015.7549794
GCACCGT1552.669385E-715.6124286
GTCCTAC3250.015.5718841
ATAGGAC2001.2769306E-915.3995323
ATCAACG23450.015.3854593
TAATACT2750.015.1995374
TATCAAC24050.015.1855872
CAACGCA23850.015.1274225
TCTAAGC1809.058749E-814.666223
GTATAGC3400.014.23772051
GTCCTAG3300.014.0023861
GGACGTG3150.013.9678296