##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062185_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1493855 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.19907889319914 32.0 32.0 32.0 32.0 32.0 2 31.451039090139272 32.0 32.0 32.0 32.0 32.0 3 31.573395677626007 32.0 32.0 32.0 32.0 32.0 4 31.627133824902685 32.0 32.0 32.0 32.0 32.0 5 31.629358271050403 32.0 32.0 32.0 32.0 32.0 6 35.23143009194333 36.0 36.0 36.0 36.0 36.0 7 35.25405143069441 36.0 36.0 36.0 36.0 36.0 8 35.196187715675215 36.0 36.0 36.0 36.0 36.0 9 35.30926696366113 36.0 36.0 36.0 36.0 36.0 10 35.17849188843629 36.0 36.0 36.0 36.0 36.0 11 35.30747964159841 36.0 36.0 36.0 36.0 36.0 12 35.241681421556976 36.0 36.0 36.0 36.0 36.0 13 35.259501089463164 36.0 36.0 36.0 36.0 36.0 14 35.20539610604778 36.0 36.0 36.0 36.0 36.0 15 35.19155808294647 36.0 36.0 36.0 36.0 36.0 16 35.22376669757105 36.0 36.0 36.0 36.0 36.0 17 35.21364791094182 36.0 36.0 36.0 36.0 36.0 18 35.192704780584464 36.0 36.0 36.0 36.0 36.0 19 35.19459117518099 36.0 36.0 36.0 36.0 36.0 20 35.19451620137162 36.0 36.0 36.0 36.0 36.0 21 35.186677421838134 36.0 36.0 36.0 36.0 36.0 22 35.167055704870954 36.0 36.0 36.0 36.0 36.0 23 35.11704683520154 36.0 36.0 36.0 36.0 36.0 24 35.13464693695171 36.0 36.0 36.0 36.0 36.0 25 35.111531574349584 36.0 36.0 36.0 36.0 36.0 26 35.081280311676835 36.0 36.0 36.0 36.0 36.0 27 35.061532745815356 36.0 36.0 36.0 36.0 36.0 28 35.040542756827136 36.0 36.0 36.0 36.0 36.0 29 35.00936302385439 36.0 36.0 36.0 36.0 36.0 30 35.00533518982766 36.0 36.0 36.0 36.0 36.0 31 34.98918502799803 36.0 36.0 36.0 36.0 36.0 32 34.93581974154118 36.0 36.0 36.0 36.0 36.0 33 34.89647924330005 36.0 36.0 36.0 32.0 36.0 34 34.88613754346975 36.0 36.0 36.0 32.0 36.0 35 34.873588802126044 36.0 36.0 36.0 32.0 36.0 36 34.845523829287316 36.0 36.0 36.0 32.0 36.0 37 34.84705008183525 36.0 36.0 36.0 32.0 36.0 38 34.84843843612666 36.0 36.0 36.0 32.0 36.0 39 34.833937028694216 36.0 36.0 36.0 32.0 36.0 40 34.83234584347209 36.0 36.0 36.0 32.0 36.0 41 34.820250961438695 36.0 36.0 36.0 32.0 36.0 42 34.803854457092555 36.0 36.0 36.0 32.0 36.0 43 34.7896422343534 36.0 36.0 36.0 32.0 36.0 44 34.75207633940376 36.0 36.0 36.0 32.0 36.0 45 34.730673994463984 36.0 36.0 36.0 32.0 36.0 46 34.69763397384619 36.0 36.0 36.0 32.0 36.0 47 34.661718172111755 36.0 36.0 36.0 32.0 36.0 48 34.63749627641237 36.0 36.0 36.0 32.0 36.0 49 34.610324964605 36.0 36.0 36.0 32.0 36.0 50 34.20836359619909 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 4.0 20 14.0 21 41.0 22 132.0 23 312.0 24 820.0 25 1817.0 26 3799.0 27 7004.0 28 12056.0 29 19246.0 30 28096.0 31 41077.0 32 61615.0 33 103286.0 34 235135.0 35 979400.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09480666017123 17.59019121374355 11.900653227129517 26.41434889895571 2 15.796619065088219 19.78828321020118 38.038128569783666 26.37696915492694 3 18.79540300058105 23.162257906448065 28.612559276604514 29.42977981636637 4 12.559652710604444 16.18242734401933 35.90917458521744 35.34874536015879 5 14.08557055403637 36.91469386252347 34.26035324713577 14.739382336304393 6 34.504355183779914 35.54830063486879 16.60050660975786 13.346837571593436 7 30.122267556088104 30.522239440909594 20.40840643837588 18.94708656462642 8 27.41611468315198 34.541839736788376 18.85417259372563 19.187872986334014 9 26.84002128720659 14.693527818965027 18.364633783064622 40.10181711076376 10 15.247875629077539 27.823200928604802 32.85970382647836 24.069219615839298 11 36.617208497477996 21.543456359552966 22.25550672588705 19.583828417081982 12 23.963436879750713 23.64165196756044 29.65562253364617 22.73928861904268 13 29.64424258043786 20.41074936991877 24.729508553373655 25.215499496269718 14 22.699257960109918 19.546475394198232 26.153676226943045 31.60059041874881 15 24.728906085262626 27.44262328003722 22.664984218682534 25.163486416017616 16 24.866686392037735 26.51886472902829 24.1883701532822 24.426078725651774 17 23.678603345036834 26.416084559746427 25.63702635128577 24.26828574393097 18 23.816281666456916 26.233924110286694 26.17334247301607 23.77645175024032 19 24.837166148766883 25.652038284865856 25.946645388371287 23.564150177995977 20 25.04874997238673 25.431653977950887 25.992755616873747 23.52684043278863 21 25.20043779349401 25.019764301086784 25.745537552172067 24.034260353247134 22 24.736001820792513 25.295493873234015 25.768699103996035 24.199805201977433 23 24.316917394147886 25.394265700842052 25.665376713773043 24.623440191237023 24 24.131767793070207 25.465913350827396 25.93236223424195 24.46995662186044 25 24.47618499338621 25.300231348838704 25.95498337180222 24.26860028597287 26 24.30943072885985 25.592365982970062 25.873922240668346 24.22428104750174 27 24.42608026240921 25.700170699869464 25.72092244870636 24.15282658901496 28 24.331798345587128 25.517079332692028 25.799977641260043 24.351144680460806 29 24.253455335847196 25.762443192049535 25.676825435502515 24.307276036600754 30 24.49950931629619 25.748644092984897 25.773680341992485 23.97816624872643 31 24.563125188690424 25.467968984817073 25.75702095727212 24.21188486922038 32 24.287723493259307 25.68191844223968 25.74966345056388 24.28069461393713 33 24.259112856636975 25.548933595117585 25.835374478445306 24.356579069800134 34 24.451047832239738 25.638854339737833 25.87428690775354 24.03581092026889 35 24.38933121978453 25.566889401507776 25.84703726066938 24.19674211803831 36 24.34503261030369 25.61075286524176 25.905427106950953 24.138787417503597 37 24.47644715068361 25.49008188337109 25.596720380872817 24.436750585072485 38 24.197194506829646 25.729404794976755 25.839589518393684 24.233811179799915 39 24.464336398348426 25.73039559580359 25.566322678077018 24.238945327770963 40 24.578841038013085 25.43655537957525 25.855137115139765 24.1294664672719 41 24.276193268614133 25.54835263261478 25.97189253453186 24.20356156423923 42 24.36413247948501 25.610161027081524 25.789035377222707 24.236671116210758 43 24.466979114297324 25.325931167354064 25.816827855458275 24.390261862890338 44 24.302945669593903 25.758241140297205 25.7188111282339 24.220002061874993 45 24.482585262892943 25.750608673997753 25.468580238194537 24.298225824914766 46 24.394121659661153 25.731562574682542 25.51808346772007 24.356232297936234 47 24.44739731162641 25.697196486906225 25.669951266534568 24.18545493493279 48 24.295131373975842 25.845350313866284 25.613925393747046 24.245592918410832 49 24.34707050313093 25.70059298195348 25.457723425933487 24.494613088982103 50 24.34877433080048 26.036977260571266 25.531902074992118 24.082346333636135 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 65.0 1 52.5 2 40.0 3 113.5 4 187.0 5 212.5 6 238.0 7 218.5 8 199.0 9 281.5 10 364.0 11 567.0 12 770.0 13 1355.0 14 1940.0 15 2746.0 16 3552.0 17 3715.0 18 3878.0 19 3908.0 20 3938.0 21 4697.5 22 5457.0 23 6693.5 24 7930.0 25 10052.5 26 12175.0 27 14728.5 28 17282.0 29 20806.5 30 24331.0 31 28058.5 32 31786.0 33 36123.5 34 40461.0 35 45646.0 36 50831.0 37 56453.5 38 62076.0 39 66650.5 40 71225.0 41 77315.0 42 83405.0 43 87093.0 44 90781.0 45 95212.0 46 99643.0 47 103168.0 48 106693.0 49 107681.0 50 108669.0 51 109000.5 52 109332.0 53 107566.0 54 105800.0 55 102604.0 56 99408.0 57 93626.5 58 87845.0 59 80982.0 60 74119.0 61 65620.5 62 57122.0 63 48689.0 64 40256.0 65 34817.0 66 29378.0 67 24768.0 68 20158.0 69 16809.0 70 13460.0 71 11227.0 72 8994.0 73 7873.5 74 6753.0 75 5722.0 76 4691.0 77 4003.5 78 3316.0 79 2824.5 80 2333.0 81 1848.5 82 1364.0 83 1090.5 84 817.0 85 611.5 86 406.0 87 287.5 88 169.0 89 137.0 90 105.0 91 76.5 92 48.0 93 37.5 94 27.0 95 20.5 96 14.0 97 16.5 98 19.0 99 12.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0334704506126766 2 0.004485040382098664 3 7.363499134788852E-4 4 0.0 5 0.0 6 2.008227036760596E-4 7 0.0 8 0.0 9 0.0 10 6.69409012253532E-5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 6.024681110281788E-4 17 0.0 18 2.008227036760596E-4 19 6.69409012253532E-5 20 5.355272098028256E-4 21 0.0 22 0.0 23 1.338818024507064E-4 24 0.001004113518380298 25 4.6858630857747233E-4 26 0.001004113518380298 27 3.34704506126766E-4 28 0.0021421088392113022 29 4.016454073521192E-4 30 0.0014057589257324172 31 4.016454073521192E-4 32 0.0012049362220563577 33 2.677636049014128E-4 34 6.024681110281788E-4 35 6.69409012253532E-5 36 1.338818024507064E-4 37 0.0015396407281831235 38 0.0 39 4.6858630857747233E-4 40 6.69409012253532E-5 41 0.0011379953208310043 42 0.004618922184549371 43 0.0038825722710704854 44 0.004618922184549371 45 0.0022759906416620086 46 0.0021421088392113022 47 0.001004113518380298 48 0.004418099480873312 49 0.0032801041600423065 50 0.0012049362220563577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1493855.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.421938174537864 #Duplication Level Percentage of deduplicated Percentage of total 1 64.02032053924457 32.28028644143886 2 17.313001060070558 17.459101381331724 3 7.648689133481855 11.569851918140447 4 3.8775422204179932 7.820527764283053 5 2.2298952200362496 5.621781946018264 6 1.3771126217778409 4.1662012484794815 7 0.8994770039650657 3.174736171834156 8 0.5826171754938644 2.35013497617404 9 0.445874650919993 2.0233677674053383 >10 1.5756319964202854 12.251622086611947 >50 0.024493981821381045 0.827602422989261 >100 0.005077209039561121 0.3379153898815263 >500 1.3359365538608543E-4 0.04156143486983221 >1k 1.3359365538608543E-4 0.07530905054209466 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0082270367605958E-4 2 0.0 0.0 0.0 0.0 2.0082270367605958E-4 3 0.0 0.0 0.0 0.0 2.0082270367605958E-4 4 0.0 0.0 0.0 0.0 2.0082270367605958E-4 5 0.0 0.0 0.0 0.0 2.677636049014128E-4 6 0.0 0.0 0.0 0.0 4.685863085774724E-4 7 0.0 0.0 0.0 0.0 4.685863085774724E-4 8 0.0 0.0 0.0 0.0 4.685863085774724E-4 9 0.0 0.0 0.0 0.0 4.685863085774724E-4 10 0.0 0.0 0.0 0.0 4.685863085774724E-4 11 0.0 0.0 0.0 0.0 4.685863085774724E-4 12 0.0 0.0 0.0 0.0 8.702317159295916E-4 13 0.0 0.0 0.0 0.0 0.0010710544196056511 14 0.0 0.0 0.0 0.0 0.0010710544196056511 15 6.69409012253532E-5 0.0 0.0 1.338818024507064E-4 0.0012718771232817108 16 6.69409012253532E-5 0.0 0.0 1.338818024507064E-4 0.0012718771232817108 17 6.69409012253532E-5 0.0 0.0 1.338818024507064E-4 0.0012718771232817108 18 6.69409012253532E-5 0.0 0.0 1.338818024507064E-4 0.001338818024507064 19 6.69409012253532E-5 0.0 0.0 1.338818024507064E-4 0.001338818024507064 20 6.69409012253532E-5 0.0 0.0 3.34704506126766E-4 0.0015396407281831235 21 6.69409012253532E-5 0.0 0.0 3.34704506126766E-4 0.0015396407281831235 22 6.69409012253532E-5 0.0 0.0 4.0164540735211917E-4 0.0016735225306338298 23 6.69409012253532E-5 0.0 0.0 5.355272098028256E-4 0.0016735225306338298 24 6.69409012253532E-5 0.0 0.0 0.0010710544196056511 0.0016735225306338298 25 6.69409012253532E-5 0.0 0.0 0.0014057589257324172 0.0016735225306338298 26 6.69409012253532E-5 0.0 0.0 0.002008227036760596 0.0017404634318591832 27 6.69409012253532E-5 0.0 0.0 0.002610695147788775 0.0017404634318591832 28 6.69409012253532E-5 0.0 0.0 0.003748690468619779 0.0017404634318591832 29 6.69409012253532E-5 0.0 0.0 0.005355272098028256 0.0017404634318591832 30 6.69409012253532E-5 0.0 0.0 0.007095735529887439 0.0017404634318591832 31 6.69409012253532E-5 0.0 0.0 0.00984031248012692 0.0018743452343098896 32 6.69409012253532E-5 0.0 0.0 0.014927820973253764 0.002075167937985949 33 6.69409012253532E-5 0.0 0.0 0.01860957054064819 0.002075167937985949 34 6.69409012253532E-5 0.0 0.0 0.02262602461416938 0.002409872444112715 35 6.69409012253532E-5 0.0 0.0 0.028182119415873695 0.002409872444112715 36 6.69409012253532E-5 0.0 0.0 0.03514397314331043 0.002409872444112715 37 6.69409012253532E-5 0.0 0.0 0.044314876611183815 0.002409872444112715 38 6.69409012253532E-5 0.0 0.0 0.05884105217708546 0.002409872444112715 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTACG 55 1.5934685E-4 24.000877 24 GTATCAA 1745 0.0 20.805838 1 GGTATCA 930 0.0 20.347555 1 ATACCGT 110 1.2665441E-7 19.999392 6 TAGGACG 195 0.0 19.178904 4 TCGTTAC 80 0.0019881607 16.500603 23 GGTCTAT 165 3.1053787E-8 16.002728 1 AACGCAG 2305 0.0 15.938778 6 TCAACGC 2290 0.0 15.754979 4 GCACCGT 155 2.669385E-7 15.612428 6 GTCCTAC 325 0.0 15.571884 1 ATAGGAC 200 1.2769306E-9 15.399532 3 ATCAACG 2345 0.0 15.385459 3 TAATACT 275 0.0 15.199537 4 TATCAAC 2405 0.0 15.185587 2 CAACGCA 2385 0.0 15.127422 5 TCTAAGC 180 9.058749E-8 14.66622 3 GTATAGC 340 0.0 14.2377205 1 GTCCTAG 330 0.0 14.002386 1 GGACGTG 315 0.0 13.967829 6 >>END_MODULE