FastQCFastQC Report
Thu 2 Feb 2017
SRR4062184_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062184_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057204
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32090.155988419233095No Hit
GCGCAAGACGGACCAGAGCGAAAGC27080.13163497640486796No Hit
GGGTAGGCACACGCTGAGCCAGTCA23810.11573961551698325No Hit
ATCAGATACCGTCGTAGTTCCGACC23300.11326052253446911No Hit
TCGTAGTTCCGACCATAAACGATGC23110.1123369388743168No Hit
GAATAGGACCGCGGTTCTATTTTGT22510.1094203588948884No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC22200.1079134592388504No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG21610.10504548892574582No Hit
GATTAAGAGGGACGGCCGGGGGCAT21290.10348997960338402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8750.015.6259381
AACCGCG1750.014.1160217
GCGTAAC2600.012.79065611
CGCGTAA2750.012.78401310
TCGCGTA2750.012.7837019
CGGTCCA3750.012.66884110
AATCGTA604.0912986E-412.66729913
GTATCAA25950.012.5135471
TATGCGC550.003065080412.09298410
AGAACCG2800.011.8758835
CGCGGTC1701.8189894E-1211.737308510
ACTCTAA3050.011.52656810
CTAAGAC1657.2759576E-1211.5132053
TAACGAA1900.011.50057413
TAGAACC2650.011.4717134
ATAACGA2000.011.401401512
GGTCCAA4200.011.31146511
CGTAACT3050.011.21449412
CGCCGGT4600.011.1535457
CGAACGT1451.2187229E-911.1379464
Produced by FastQC (version 0.11.5)