##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062184_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2057204
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.267796484937808	32.0	32.0	32.0	32.0	32.0
2	30.814274131296653	32.0	32.0	32.0	32.0	32.0
3	30.835675509089036	32.0	32.0	32.0	32.0	32.0
4	30.837018594169564	32.0	32.0	32.0	32.0	32.0
5	30.754881382692236	32.0	32.0	32.0	32.0	32.0
6	34.41754877007822	36.0	36.0	36.0	32.0	36.0
7	34.3531895718655	36.0	36.0	36.0	32.0	36.0
8	34.317338484661704	36.0	36.0	36.0	32.0	36.0
9	34.44493642827838	36.0	36.0	36.0	32.0	36.0
10	34.13407080678435	36.0	36.0	36.0	32.0	36.0
11	34.42851754128419	36.0	36.0	36.0	32.0	36.0
12	34.244495927482156	36.0	36.0	36.0	32.0	36.0
13	34.337758919387674	36.0	36.0	36.0	32.0	36.0
14	34.23375805219123	36.0	36.0	36.0	32.0	36.0
15	34.18344413096611	36.0	36.0	36.0	32.0	36.0
16	34.18647251317808	36.0	36.0	36.0	32.0	36.0
17	34.119205970822534	36.0	36.0	36.0	32.0	36.0
18	34.13099818977603	36.0	36.0	36.0	32.0	36.0
19	34.13024960091464	36.0	36.0	36.0	32.0	36.0
20	34.12233400285047	36.0	36.0	36.0	32.0	36.0
21	34.10449765798628	36.0	36.0	36.0	32.0	36.0
22	34.07516172435986	36.0	36.0	36.0	32.0	36.0
23	34.040265331002665	36.0	36.0	36.0	32.0	36.0
24	34.01688116492093	36.0	36.0	36.0	32.0	36.0
25	33.66983974365206	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	36.0
5	147.0
6	414.0
7	87.0
8	374.0
9	291.0
10	171.0
11	36.0
12	93.0
13	84.0
14	296.0
15	393.0
16	693.0
17	1029.0
18	1343.0
19	1796.0
20	2674.0
21	3978.0
22	6146.0
23	8849.0
24	12411.0
25	17711.0
26	23803.0
27	30813.0
28	40422.0
29	52934.0
30	69172.0
31	93389.0
32	133744.0
33	190456.0
34	420916.0
35	942503.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3508556595542	17.290763835046434	11.85800013124731	25.50038037415206
2	17.255047027490264	18.889500831469554	36.986079629554915	26.869372511485267
3	19.592492870132137	22.28932559008862	27.63413800663137	30.48404353314787
4	13.320152203642133	15.240782486566479	34.09909960276736	37.33996570702403
5	15.684260840939581	35.62568653165303	32.49118783895031	16.19886478845708
6	35.72282552125133	33.9168196396938	16.17618017839233	14.184174660662535
7	31.7108896205582	29.549053366333066	19.376670952220135	19.363386060888597
8	28.85631697275833	32.34162554624466	18.48224279054376	20.31981469045325
9	27.002287438555506	14.737479924076508	18.57400107071592	39.68623156665206
10	17.42887808819628	25.939218115308886	29.518714755438953	27.113189041055886
11	37.39261953568705	21.224518208737837	21.314477566385186	20.068384689189934
12	25.07402357753772	23.40503102346023	27.492914034325132	24.028031364676917
13	29.36143432453559	19.11248346346777	24.833807242684745	26.692274969311896
14	24.042922544653393	19.432676439369644	24.181429463429257	32.34297155254771
15	25.64765918838105	26.677235661156807	21.590781318677575	26.084323831784573
16	26.556024069412732	25.59531109774354	22.71468090118186	25.13398393166187
17	24.551878608004447	25.931930422610755	24.247882711900374	25.268308257484428
18	25.28875137355819	24.825610424345534	25.219647648867426	24.66599055322885
19	25.989965681287032	24.72402217456951	24.616779237689567	24.669232906453896
20	26.32090591419038	23.906604116004814	24.161409511242674	25.611080458562135
21	27.283205776313807	23.779417989675423	23.935892258513398	25.001483975497372
22	26.61942443172584	23.677887305713348	24.423940261264516	25.278748001296297
23	24.9457064294096	23.996541947134798	25.05638552716333	26.001366096292273
24	25.065378827428603	24.67964982735124	24.75029545148246	25.504675893737694
25	25.347748961010275	24.136380841914953	24.600581308791348	25.915288888283428
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	218.0
1	218.0
2	391.0
3	564.0
4	564.0
5	564.0
6	1170.5
7	1777.0
8	1777.0
9	1777.0
10	2206.5
11	2636.0
12	2636.0
13	2636.0
14	3561.5
15	4487.0
16	4487.0
17	4487.0
18	7327.0
19	10167.0
20	10167.0
21	10167.0
22	16439.0
23	22711.0
24	22711.0
25	22711.0
26	34089.0
27	45467.0
28	45467.0
29	45467.0
30	57802.0
31	70137.0
32	70137.0
33	70137.0
34	90208.5
35	110280.0
36	110280.0
37	110280.0
38	132752.0
39	155224.0
40	155224.0
41	155224.0
42	182160.5
43	209097.0
44	209097.0
45	209097.0
46	235863.0
47	262629.0
48	262629.0
49	262629.0
50	276003.5
51	289378.0
52	289378.0
53	289378.0
54	273140.0
55	256902.0
56	256902.0
57	256902.0
58	239176.0
59	221450.0
60	221450.0
61	221450.0
62	195356.0
63	169262.0
64	169262.0
65	169262.0
66	139082.0
67	108902.0
68	108902.0
69	108902.0
70	82751.5
71	56601.0
72	56601.0
73	56601.0
74	43168.0
75	29735.0
76	29735.0
77	29735.0
78	23678.5
79	17622.0
80	17622.0
81	17622.0
82	12213.0
83	6804.0
84	6804.0
85	6804.0
86	4974.0
87	3144.0
88	3144.0
89	3144.0
90	2062.5
91	981.0
92	981.0
93	981.0
94	613.5
95	246.0
96	246.0
97	246.0
98	514.5
99	783.0
100	783.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.235836601523233E-4
2	1.4582899897141945E-4
3	6.319256622094844E-4
4	0.010839955590208847
5	0.03261708610327415
6	0.06251203089241514
7	0.10878843323267892
8	0.1100522845570979
9	0.12171860447481143
10	0.1365445527035724
11	0.1430582479909625
12	0.13566957870974392
13	0.12939893175397285
14	0.11826731816582119
15	0.13450294671797255
16	0.12055197248304009
17	0.12497545211850648
18	0.11287164520387867
19	0.09999008362806994
20	0.0928444626784704
21	0.09303890134376562
22	0.10314971193911737
23	0.08321974874635671
24	0.09114312435713716
25	0.08953900536845155
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2057204.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.56561558288105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.68551242329983	35.04896757954285
2	14.41512130544062	13.71328237190374
3	4.919224916127886	7.019578839788078
4	2.259473866803868	4.298930614718342
5	1.2069939873452986	2.870570600645763
6	0.756739244535106	2.1596860801222
7	0.4895916320883002	1.630140915515519
8	0.35351621420314644	1.3452173077681837
9	0.2707681929826624	1.1591330201536203
>10	1.4557821145527596	12.438032816485837
>50	0.09462516488728448	3.097438795422564
>100	0.07592865515070703	7.597229265230762
>500	0.01138756666670104	3.67989795109508
>1k	0.005334715915932017	3.9418938416074654
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3209	0.155988419233095	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2708	0.13163497640486796	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2381	0.11573961551698325	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2330	0.11326052253446911	No Hit
TCGTAGTTCCGACCATAAACGATGC	2311	0.1123369388743168	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2251	0.1094203588948884	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2220	0.1079134592388504	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2161	0.10504548892574582	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2129	0.10348997960338402	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.860966632380649E-5
6	0.0	0.0	0.0	0.0	1.4582899897141945E-4
7	4.860966632380649E-5	0.0	0.0	0.0	1.4582899897141945E-4
8	4.860966632380649E-5	0.0	0.0	0.0	1.4582899897141945E-4
9	4.860966632380649E-5	0.0	0.0	0.0	1.4582899897141945E-4
10	4.860966632380649E-5	0.0	0.0	0.0	1.4582899897141945E-4
11	4.860966632380649E-5	0.0	4.860966632380649E-5	0.0	1.4582899897141945E-4
12	4.860966632380649E-5	0.0	4.860966632380649E-5	0.0	4.374869969142584E-4
13	4.860966632380649E-5	0.0	4.860966632380649E-5	0.0	5.347063295618713E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	875	0.0	15.625938	1
AACCGCG	175	0.0	14.116021	7
GCGTAAC	260	0.0	12.790656	11
CGCGTAA	275	0.0	12.784013	10
TCGCGTA	275	0.0	12.783701	9
CGGTCCA	375	0.0	12.668841	10
AATCGTA	60	4.0912986E-4	12.667299	13
GTATCAA	2595	0.0	12.513547	1
TATGCGC	55	0.0030650804	12.092984	10
AGAACCG	280	0.0	11.875883	5
CGCGGTC	170	1.8189894E-12	11.7373085	10
ACTCTAA	305	0.0	11.526568	10
CTAAGAC	165	7.2759576E-12	11.513205	3
TAACGAA	190	0.0	11.500574	13
TAGAACC	265	0.0	11.471713	4
ATAACGA	200	0.0	11.4014015	12
GGTCCAA	420	0.0	11.311465	11
CGTAACT	305	0.0	11.214494	12
CGCCGGT	460	0.0	11.153545	7
CGAACGT	145	1.2187229E-9	11.137946	4
>>END_MODULE