FastQCFastQC Report
Thu 2 Feb 2017
SRR4062184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057204
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26730.12993363808353472No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC24730.12021170481877344No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23440.1139410578630024No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT23070.11214250020902157No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA22880.11121891654886924No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22330.10854538490105989No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG22300.10839955590208847No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA21860.10626073058384099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11900.027.919051
GTATCAA29000.027.009881
TAATCGG856.11874E-620.7051515
ATACGAA5550.019.4255740
GTCTACG1303.5863195E-818.6174411
GTATTAC2250.018.5798321
CCTTATA1801.4551915E-1118.3326842
CGCAATA6400.017.8765436
TAATACC1852.1827873E-1117.8367754
TACGAAT6050.017.8201541
CGAATGC6350.017.67124443
TCAACGC45050.017.2864274
ATCAACG45900.017.1100923
ATCGTTT6600.016.999429
CAACGCA45950.016.9957225
TATCAAC47400.016.9403292
CGTAATC800.001989783316.49901613
AACGCAG47500.016.4874386
CAATACG6550.016.45983538
TCGTTTA6700.016.4173330
Produced by FastQC (version 0.11.5)