##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062184_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2057204
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.331676877937237	32.0	32.0	32.0	32.0	32.0
2	31.50496013035168	32.0	32.0	32.0	32.0	32.0
3	31.60501340654597	32.0	32.0	32.0	32.0	32.0
4	31.673250683938004	32.0	32.0	32.0	32.0	32.0
5	31.645626782759514	32.0	32.0	32.0	32.0	32.0
6	35.30625791122319	36.0	36.0	36.0	36.0	36.0
7	35.30574702363013	36.0	36.0	36.0	36.0	36.0
8	35.26525662987239	36.0	36.0	36.0	36.0	36.0
9	35.33286295379554	36.0	36.0	36.0	36.0	36.0
10	35.22381640323468	36.0	36.0	36.0	36.0	36.0
11	35.33799370407602	36.0	36.0	36.0	36.0	36.0
12	35.26019393312477	36.0	36.0	36.0	36.0	36.0
13	35.29479915458068	36.0	36.0	36.0	36.0	36.0
14	35.25325441716038	36.0	36.0	36.0	36.0	36.0
15	35.23575396509048	36.0	36.0	36.0	36.0	36.0
16	35.24443030443262	36.0	36.0	36.0	36.0	36.0
17	35.22621140149445	36.0	36.0	36.0	36.0	36.0
18	35.22255935726355	36.0	36.0	36.0	36.0	36.0
19	35.21481729570816	36.0	36.0	36.0	36.0	36.0
20	35.215893513720566	36.0	36.0	36.0	36.0	36.0
21	35.20722592411837	36.0	36.0	36.0	36.0	36.0
22	35.19473178158316	36.0	36.0	36.0	36.0	36.0
23	35.16765328086082	36.0	36.0	36.0	36.0	36.0
24	35.14861384675511	36.0	36.0	36.0	36.0	36.0
25	35.13085624955036	36.0	36.0	36.0	36.0	36.0
26	35.091881019091936	36.0	36.0	36.0	36.0	36.0
27	35.08343411737484	36.0	36.0	36.0	36.0	36.0
28	35.06344679477582	36.0	36.0	36.0	36.0	36.0
29	35.04176591140208	36.0	36.0	36.0	36.0	36.0
30	35.027675913521456	36.0	36.0	36.0	36.0	36.0
31	35.026770315437844	36.0	36.0	36.0	36.0	36.0
32	35.006063083680566	36.0	36.0	36.0	36.0	36.0
33	34.984379769823505	36.0	36.0	36.0	36.0	36.0
34	34.96787095494662	36.0	36.0	36.0	36.0	36.0
35	34.9608881763792	36.0	36.0	36.0	36.0	36.0
36	34.939716236211865	36.0	36.0	36.0	36.0	36.0
37	34.92987326487796	36.0	36.0	36.0	36.0	36.0
38	34.911676236289644	36.0	36.0	36.0	36.0	36.0
39	34.90329690200875	36.0	36.0	36.0	32.0	36.0
40	34.88841408047038	36.0	36.0	36.0	32.0	36.0
41	34.85900134357118	36.0	36.0	36.0	32.0	36.0
42	34.82461389342039	36.0	36.0	36.0	32.0	36.0
43	34.82392898322189	36.0	36.0	36.0	32.0	36.0
44	34.78421391364201	36.0	36.0	36.0	32.0	36.0
45	34.77387804029158	36.0	36.0	36.0	32.0	36.0
46	34.75480214893613	36.0	36.0	36.0	32.0	36.0
47	34.732130600562705	36.0	36.0	36.0	32.0	36.0
48	34.699377893490386	36.0	36.0	36.0	32.0	36.0
49	34.69389277874241	36.0	36.0	36.0	32.0	36.0
50	34.29327281105812	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	8.0
20	16.0
21	44.0
22	161.0
23	412.0
24	973.0
25	2153.0
26	4853.0
27	8889.0
28	15021.0
29	24053.0
30	36353.0
31	53135.0
32	80832.0
33	136856.0
34	318735.0
35	1374709.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.98240132106794	17.778643318463537	12.27390180878553	25.965053551682992
2	16.58294144354794	19.424721674802615	36.70217575394021	27.29016112770924
3	18.911557813860316	22.675125425518313	28.02604709223278	30.38726966838859
4	13.039597939532463	15.580620872125891	34.28052554852389	37.099255639817756
5	15.330176297537824	35.92414753228168	32.42809172060719	16.317584449573307
6	35.816286303574614	34.115771866725716	16.04495622936213	14.022985600337545
7	31.732827663177787	29.55307300588566	19.392340283219358	19.321759047717194
8	28.928195745293127	32.333400090608414	18.441632429258352	20.296771734840103
9	27.057890223818347	14.524033591223818	18.50915125578212	39.90892492917572
10	17.427205774053412	25.929769692149975	29.57739221651923	27.065632317277387
11	37.628548262593306	21.08682464160093	21.34324063145901	19.941386464346756
12	25.013659316236993	23.319029128856446	27.538688433427115	24.128623121479446
13	29.453277360922886	19.008226699928642	24.947452950703966	26.591042988444507
14	24.139317248070682	19.281315805335787	24.2059124909343	32.373454455659235
15	25.698861172737363	26.581029397181805	21.571803282513546	26.14830614756728
16	26.58067558174014	25.51329726471546	22.711660080012056	25.19436707353234
17	24.646316067827982	25.84896782234528	24.227592402114713	25.27712370771202
18	25.49518667084062	24.616178074707225	25.266137923122837	24.622497331329317
19	26.150688021217146	24.609810208418807	24.67139865565107	24.56810311471298
20	26.476757406815988	23.778469010928973	24.200111511170803	25.54466207108424
21	27.397711745230534	23.700503742706715	24.004363210012052	24.8974213020507
22	26.71110886426431	23.603930383180277	24.598727204496978	25.086233548058434
23	25.04581465505089	23.826196941282383	25.144297934767707	25.983690468899017
24	25.167984732505307	24.5048442504573	24.842150496237327	25.485020520800067
25	25.52172856309547	23.87944779311686	24.754472097997276	25.844351545790396
26	25.151091950139776	25.002150991327493	25.01449792459718	24.832259133935548
27	25.796812749003983	24.174653265901743	24.602614432460495	25.42591955263378
28	25.291711145203223	24.091762866692722	25.16036578370695	25.456160204397104
29	25.124307613036194	24.403617547194116	25.133154611011594	25.338920228758088
30	25.08613750111804	24.527799274345025	25.29545357324799	25.090609651288954
31	25.77922851261062	24.15225586295903	24.315147567440132	25.753368056990222
32	25.63738984308123	24.213255142114516	24.096687745694467	26.052667269109786
33	25.161773598626482	24.024448812850114	24.73629153469091	26.077486053832498
34	25.942791810957484	24.13945638415157	24.747713869612305	25.170037935278643
35	26.209983778899154	23.847886225772253	25.039604860399855	24.90252513492874
36	24.984226147942692	24.633944551871913	24.495261039022907	25.88656826116249
37	26.09670602955595	24.24101307467542	24.54016001493304	25.122120880835592
38	25.27109610908787	23.912067058006887	24.86107357364656	25.955763259258685
39	26.06131164036467	24.01036362619975	24.259392036243526	25.668932697192048
40	25.998953918963718	24.25046252142473	24.67531141813006	25.075272141481484
41	25.07414265041078	24.8240433641538	25.04726123052738	25.05455275490805
42	26.40977914193338	24.555724454611877	24.426368410485956	24.608127992968786
43	25.893300290357534	23.792303702410994	24.51399262755339	25.800403379678084
44	25.23483153331878	24.443188099049493	24.428458718602236	25.893521649029488
45	25.469602355862385	24.537203123131533	24.508279837407386	25.484914683598696
46	25.401888621314217	24.48236940017364	24.399050731055695	25.716691247456446
47	25.437249049669937	24.33856055376778	24.970590808777064	25.25359958778522
48	26.167129854908733	24.903420754949032	23.817338714969818	25.11211067517241
49	25.375657774807074	24.94306374187276	24.024402989609285	25.656875493710885
50	25.64907003491197	25.01125330185974	24.167863292069605	25.171813371158684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	60.5
2	65.0
3	175.5
4	286.0
5	279.0
6	272.0
7	219.0
8	166.0
9	243.0
10	320.0
11	578.5
12	837.0
13	1523.5
14	2210.0
15	2897.5
16	3585.0
17	3512.0
18	3439.0
19	3291.5
20	3144.0
21	3630.5
22	4117.0
23	4839.5
24	5562.0
25	7199.5
26	8837.0
27	11306.0
28	13775.0
29	16204.5
30	18634.0
31	21804.0
32	24974.0
33	28754.0
34	32534.0
35	38283.5
36	44033.0
37	53819.5
38	63606.0
39	72116.0
40	80626.0
41	89031.0
42	97436.0
43	102359.5
44	107283.0
45	119225.0
46	131167.0
47	141405.0
48	151643.0
49	159068.5
50	166494.0
51	163160.0
52	159826.0
53	160248.5
54	160671.0
55	162403.0
56	164135.0
57	158350.0
58	152565.0
59	141368.0
60	130171.0
61	114514.0
62	98857.0
63	84947.5
64	71038.0
65	60432.5
66	49827.0
67	42591.0
68	35355.0
69	31417.5
70	27480.0
71	21233.0
72	14986.0
73	13173.5
74	11361.0
75	8273.5
76	5186.0
77	4612.5
78	4039.0
79	3412.0
80	2785.0
81	2194.0
82	1603.0
83	1377.5
84	1152.0
85	897.0
86	642.0
87	431.5
88	221.0
89	151.0
90	81.0
91	58.5
92	36.0
93	32.0
94	28.0
95	24.0
96	20.0
97	25.0
98	30.0
99	19.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03334623109813125
2	0.003791553973256906
3	3.4026766426664543E-4
4	4.860966632380649E-5
5	0.0
6	1.4582899897141945E-4
7	0.0
8	0.0
9	0.0
10	4.860966632380649E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.805353285332909E-4
17	0.0
18	0.0
19	0.0
20	5.347063295618714E-4
21	1.4582899897141945E-4
22	0.0
23	9.721933264761298E-5
24	0.0011180223254475493
25	1.9443866529522596E-4
26	6.319256622094844E-4
27	3.888773305904519E-4
28	0.0015555093223618077
29	4.3748699691425834E-4
30	0.0013610706570665817
31	4.3748699691425834E-4
32	0.0012152416580951623
33	3.888773305904519E-4
34	7.777546611809038E-4
35	3.4026766426664543E-4
36	9.721933264761298E-5
37	0.0010208029927999361
38	0.0
39	4.3748699691425834E-4
40	9.721933264761298E-5
41	0.0010208029927999361
42	0.004423479635466391
43	0.004131821637523551
44	0.004423479635466391
45	0.0018471673203046466
46	0.0020416059855998723
47	0.0011666319917713556
48	0.004131821637523551
49	0.0038401636395807128
50	0.0014582899897141945
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2057204.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.38478278595695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7126791976382	35.18187188762695
2	15.090549458742498	14.603059153639919
3	5.171671052176968	7.50690541500012
4	2.2841963389107782	4.4208137479470455
5	1.2442132982699803	3.0100495088096033
6	0.7644528146245672	2.219273003143386
7	0.5072037399569607	1.7178659950229773
8	0.36793143291647257	1.4241825969431532
9	0.2690006891072743	1.1713985920555383
>10	1.4160148145709146	12.124739307896656
>50	0.08792236229309065	2.920038981785321
>100	0.06970122298375434	7.041213667475314
>500	0.010013246175204424	3.343217005353522
>1k	0.0044503316334241864	3.3153711373004415
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2673	0.12993363808353472	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2473	0.12021170481877344	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2344	0.1139410578630024	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2307	0.11214250020902157	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2288	0.11121891654886924	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2233	0.10854538490105989	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2230	0.10839955590208847	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	2186	0.10626073058384099	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4582899897141945E-4
2	0.0	0.0	0.0	0.0	1.4582899897141945E-4
3	0.0	0.0	0.0	0.0	1.4582899897141945E-4
4	0.0	0.0	0.0	0.0	1.4582899897141945E-4
5	0.0	0.0	0.0	0.0	1.9443866529522596E-4
6	0.0	0.0	0.0	0.0	1.9443866529522596E-4
7	0.0	0.0	0.0	0.0	1.9443866529522596E-4
8	0.0	0.0	0.0	0.0	1.9443866529522596E-4
9	0.0	0.0	0.0	0.0	1.9443866529522596E-4
10	0.0	0.0	0.0	0.0	1.9443866529522596E-4
11	0.0	0.0	0.0	0.0	1.9443866529522596E-4
12	0.0	0.0	0.0	0.0	3.4026766426664543E-4
13	0.0	0.0	0.0	0.0	4.374869969142584E-4
14	0.0	0.0	0.0	0.0	4.860966632380649E-4
15	0.0	0.0	0.0	1.4582899897141945E-4	5.833159958856778E-4
16	0.0	0.0	0.0	1.4582899897141945E-4	6.319256622094844E-4
17	0.0	0.0	0.0	1.9443866529522596E-4	6.805353285332909E-4
18	0.0	0.0	0.0	1.9443866529522596E-4	6.805353285332909E-4
19	0.0	0.0	0.0	2.4304833161903244E-4	6.805353285332909E-4
20	0.0	0.0	0.0	3.888773305904519E-4	7.777546611809038E-4
21	0.0	0.0	0.0	4.860966632380649E-4	8.749739938285168E-4
22	0.0	0.0	0.0	7.777546611809038E-4	9.235836601523233E-4
23	0.0	0.0	0.0	9.721933264761298E-4	9.721933264761298E-4
24	0.0	0.0	0.0	0.0011666319917713556	9.721933264761298E-4
25	0.0	0.0	0.0	0.0015068996560380012	9.721933264761298E-4
26	0.0	0.0	0.0	0.0017013383213332271	0.0011666319917713556
27	0.0	0.0	0.0	0.001895776986628453	0.001215241658095162
28	4.860966632380649E-5	0.0	0.0	0.002430483316190324	0.001215241658095162
29	4.860966632380649E-5	0.0	0.0	0.0033540669763426475	0.001215241658095162
30	4.860966632380649E-5	0.0	0.0	0.005104014963999681	0.001215241658095162
31	4.860966632380649E-5	0.0	0.0	0.009041397936228006	0.0013124609907427752
32	4.860966632380649E-5	0.0	0.0	0.012978780908456332	0.0013610706570665817
33	1.4582899897141945E-4	0.0	0.0	0.016527286550094205	0.0014582899897141947
34	1.4582899897141945E-4	0.0	0.0	0.022457665841598598	0.0015555093223618077
35	1.4582899897141945E-4	0.0	0.0	0.027999167802512537	0.0015555093223618077
36	1.4582899897141945E-4	0.0	0.0	0.035533666082702545	0.0015555093223618077
37	1.4582899897141945E-4	0.0	0.0	0.04666527967085423	0.0015555093223618077
38	1.4582899897141945E-4	0.0	0.0	0.06153983756593901	0.0015555093223618077
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1190	0.0	27.91905	1
GTATCAA	2900	0.0	27.00988	1
TAATCGG	85	6.11874E-6	20.70515	15
ATACGAA	555	0.0	19.42557	40
GTCTACG	130	3.5863195E-8	18.617441	1
GTATTAC	225	0.0	18.579832	1
CCTTATA	180	1.4551915E-11	18.332684	2
CGCAATA	640	0.0	17.87654	36
TAATACC	185	2.1827873E-11	17.836775	4
TACGAAT	605	0.0	17.82015	41
CGAATGC	635	0.0	17.671244	43
TCAACGC	4505	0.0	17.286427	4
ATCAACG	4590	0.0	17.110092	3
ATCGTTT	660	0.0	16.9994	29
CAACGCA	4595	0.0	16.995722	5
TATCAAC	4740	0.0	16.940329	2
CGTAATC	80	0.0019897833	16.499016	13
AACGCAG	4750	0.0	16.487438	6
CAATACG	655	0.0	16.459835	38
TCGTTTA	670	0.0	16.41733	30
>>END_MODULE