FastQCFastQC Report
Thu 2 Feb 2017
SRR4062183_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062183_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324816
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20800.15700293474716487No Hit
GCGCAAGACGGACCAGAGCGAAAGC15540.11729930797937221No Hit
GGGTAGGCACACGCTGAGCCAGTCA13900.10492023043199962No Hit
GAATAGGACCGCGGTTCTATTTTGT13860.10461830171133199No Hit
TATCAACGCAGAGTACTTTTTTTTT13790.10408992645016364No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTC250.00602870519.00158713
TGCGATT350.002168211216.28769112
CAAGACG2650.014.3391944
TCGCGTA1400.014.2506529
GGTATCA6250.014.1281011
CGCGTAA1350.014.07578210
TGTTCGC500.001497971413.30161510
GTCGCGT1650.012.6672478
CTAAGGT604.0923394E-412.6662894
CGTTATT1207.421477E-1012.6648532
CGCAAGA2850.012.6648532
GCGTTAT1352.7284841E-1112.6595891
AAGACGG3200.012.4688495
TACGGAG550.003071323712.0891782
GTCCGGA550.003080900312.0841541
GTATCAA16050.012.00886251
GCGTAAC1603.6379788E-1211.87733811
CGGTCCA2400.011.87644210
GTATAAA1201.0088115E-811.8683651
TTTAGAC907.4668587E-611.6098873
Produced by FastQC (version 0.11.5)