##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062183_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1324816
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234429535875172	32.0	32.0	32.0	32.0	32.0
2	30.780210987790003	32.0	32.0	32.0	32.0	32.0
3	30.811525525054044	32.0	32.0	32.0	32.0	32.0
4	30.811422869289018	32.0	32.0	32.0	32.0	32.0
5	30.732904041014	32.0	32.0	32.0	32.0	32.0
6	34.38351061581382	36.0	36.0	36.0	32.0	36.0
7	34.319630801560365	36.0	36.0	36.0	32.0	36.0
8	34.265655758988416	36.0	36.0	36.0	32.0	36.0
9	34.401227793142596	36.0	36.0	36.0	32.0	36.0
10	34.09578235770099	36.0	36.0	36.0	32.0	36.0
11	34.389007228173575	36.0	36.0	36.0	32.0	36.0
12	34.20747560415937	36.0	36.0	36.0	32.0	36.0
13	34.296383799712565	36.0	36.0	36.0	32.0	36.0
14	34.19676619243729	36.0	36.0	36.0	32.0	36.0
15	34.14780241180662	36.0	36.0	36.0	32.0	36.0
16	34.15671459281893	36.0	36.0	36.0	32.0	36.0
17	34.085079739375125	36.0	36.0	36.0	32.0	36.0
18	34.08753517469596	36.0	36.0	36.0	32.0	36.0
19	34.096195245226504	36.0	36.0	36.0	32.0	36.0
20	34.08311040929458	36.0	36.0	36.0	32.0	36.0
21	34.07461036098598	36.0	36.0	36.0	32.0	36.0
22	34.03471123537155	36.0	36.0	36.0	32.0	36.0
23	34.00219502179925	36.0	36.0	36.0	32.0	36.0
24	33.97650994553206	36.0	36.0	36.0	32.0	36.0
25	33.61502125578193	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	21.0
5	113.0
6	290.0
7	60.0
8	200.0
9	181.0
10	108.0
11	21.0
12	54.0
13	69.0
14	216.0
15	313.0
16	458.0
17	643.0
18	970.0
19	1140.0
20	1788.0
21	2595.0
22	3787.0
23	5641.0
24	8140.0
25	11355.0
26	15654.0
27	20119.0
28	27055.0
29	35476.0
30	46202.0
31	62601.0
32	87997.0
33	124961.0
34	271812.0
35	594773.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.564777884124744	17.209564584647993	11.51709988798343	25.70855764324383
2	16.844742382120923	19.141145416214087	37.44941731311108	26.564694888553912
3	19.519176334719695	22.708992232848484	27.977370339897796	29.794461092534025
4	12.918596715846434	15.236090057554216	35.02744846995163	36.81786475664772
5	15.019194292756005	36.21091673512782	33.302248948919924	15.46764002319625
6	35.26344292718224	34.65722622246023	16.26127592528111	13.81805492507642
7	30.96801493089926	29.95156525090107	19.82930717907256	19.251112639127115
8	28.2848204570185	33.33257768105429	18.714711038568492	19.667890823358725
9	26.822472283729965	14.576075209903042	18.479856109671033	40.12159639669596
10	16.580691924723162	26.577268051483564	30.618647789258713	26.223392234534558
11	37.28713501435792	21.36953187655286	21.639909808513163	19.703423300576056
12	24.577145487347433	23.564970209909976	28.313774336005608	23.544109966736983
13	29.51993989990371	19.395960777733944	24.84963639139432	26.23446293096803
14	23.407324674225503	19.69292107355204	24.711480622802853	32.188273629419605
15	25.126296981570164	27.161711531949884	21.786418275044745	25.925573211435204
16	25.89347250435287	25.99881807409557	23.18003059102341	24.927678830528148
17	24.182069503403525	26.250031174731912	24.71759471639307	24.85030460547149
18	24.869989042959155	25.198624702459664	25.68050780216874	24.25087845241244
19	25.732768586112115	25.074081517624673	24.895696547597282	24.29745334866593
20	25.809688152578282	24.548200508312203	24.587487420632883	25.054623918476636
21	26.564044060984273	24.327677127876584	24.30697632250948	24.801302488629666
22	26.060707722810584	24.25762548962716	24.803317979592823	24.878348807969438
23	24.504041173968783	24.525948650386137	25.32579816868606	25.644212006959023
24	24.68235601663074	24.940974166139686	25.176473077042218	25.20019674018736
25	24.910133224944207	24.626367266886568	25.016507684194778	25.446991823974447
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	387.0
1	387.0
2	416.0
3	445.0
4	445.0
5	445.0
6	858.0
7	1271.0
8	1271.0
9	1271.0
10	1661.0
11	2051.0
12	2051.0
13	2051.0
14	2847.0
15	3643.0
16	3643.0
17	3643.0
18	5997.0
19	8351.0
20	8351.0
21	8351.0
22	13039.5
23	17728.0
24	17728.0
25	17728.0
26	25897.5
27	34067.0
28	34067.0
29	34067.0
30	42866.0
31	51665.0
32	51665.0
33	51665.0
34	65063.0
35	78461.0
36	78461.0
37	78461.0
38	91906.0
39	105351.0
40	105351.0
41	105351.0
42	121678.5
43	138006.0
44	138006.0
45	138006.0
46	153306.5
47	168607.0
48	168607.0
49	168607.0
50	175085.0
51	181563.0
52	181563.0
53	181563.0
54	171105.5
55	160648.0
56	160648.0
57	160648.0
58	147617.0
59	134586.0
60	134586.0
61	134586.0
62	119269.0
63	103952.0
64	103952.0
65	103952.0
66	84895.5
67	65839.0
68	65839.0
69	65839.0
70	49741.0
71	33643.0
72	33643.0
73	33643.0
74	25539.5
75	17436.0
76	17436.0
77	17436.0
78	13711.0
79	9986.0
80	9986.0
81	9986.0
82	6951.5
83	3917.0
84	3917.0
85	3917.0
86	2928.0
87	1939.0
88	1939.0
89	1939.0
90	1277.5
91	616.0
92	616.0
93	616.0
94	374.0
95	132.0
96	132.0
97	132.0
98	329.0
99	526.0
100	526.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.057861620028744E-4
2	3.77410900834531E-4
3	4.528930810014372E-4
4	0.011473291385369743
5	0.03502373159744447
6	0.06544305020470767
7	0.10461830171133199
8	0.10990205432301542
9	0.11971473774471324
10	0.13232026183258658
11	0.13986847984927717
12	0.13020676078791318
13	0.12756488448207148
14	0.11382712769169455
15	0.1316409222110844
16	0.11714834361903842
17	0.12296047149189018
18	0.11065687612468449
19	0.0961642975326385
20	0.09208825980362556
21	0.09027668747961981
22	0.10265576502699243
23	0.08053948623808892
24	0.0938243499474644
25	0.08982379439861837
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1324816.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.24929909450902
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55619393707852	41.53119862429811
2	13.700608950712844	14.86496865488257
3	4.297872585947473	6.994697261554661
4	1.8717038127487349	4.061544798165561
5	0.9441815239620711	2.5610592946463866
6	0.5794205901791745	1.8859896538888186
7	0.3959204907657929	1.5034886384838808
8	0.27337250292863335	1.1864213340471979
9	0.19776079940882216	0.9655546280668557
>10	1.0314995092817876	9.96931057241298
>50	0.0747455273018415	2.800750069991822
>100	0.06819537504032425	7.691188610482334
>500	0.0064282320266082485	2.5008280383499946
>1k	0.0020961626173722553	1.4829998207288286
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2080	0.15700293474716487	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1554	0.11729930797937221	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1390	0.10492023043199962	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1386	0.10461830171133199	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1379	0.10408992645016364	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2644654050071858E-4
2	0.0	0.0	0.0	0.0	2.2644654050071858E-4
3	0.0	0.0	0.0	0.0	2.2644654050071858E-4
4	0.0	0.0	0.0	0.0	2.2644654050071858E-4
5	0.0	0.0	0.0	0.0	2.2644654050071858E-4
6	0.0	0.0	0.0	0.0	3.019287206676248E-4
7	0.0	0.0	0.0	0.0	3.019287206676248E-4
8	0.0	0.0	0.0	0.0	3.019287206676248E-4
9	0.0	0.0	0.0	0.0	3.019287206676248E-4
10	0.0	0.0	0.0	0.0	3.019287206676248E-4
11	0.0	0.0	0.0	0.0	3.019287206676248E-4
12	0.0	0.0	0.0	0.0	9.057861620028743E-4
13	0.0	0.0	0.0	0.0	9.812683421697806E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATTC	25	0.006028705	19.001587	13
TGCGATT	35	0.0021682112	16.287691	12
CAAGACG	265	0.0	14.339194	4
TCGCGTA	140	0.0	14.250652	9
GGTATCA	625	0.0	14.128101	1
CGCGTAA	135	0.0	14.075782	10
TGTTCGC	50	0.0014979714	13.301615	10
GTCGCGT	165	0.0	12.667247	8
CTAAGGT	60	4.0923394E-4	12.666289	4
CGTTATT	120	7.421477E-10	12.664853	2
CGCAAGA	285	0.0	12.664853	2
GCGTTAT	135	2.7284841E-11	12.659589	1
AAGACGG	320	0.0	12.468849	5
TACGGAG	55	0.0030713237	12.089178	2
GTCCGGA	55	0.0030809003	12.084154	1
GTATCAA	1605	0.0	12.0088625	1
GCGTAAC	160	3.6379788E-12	11.877338	11
CGGTCCA	240	0.0	11.876442	10
GTATAAA	120	1.0088115E-8	11.868365	1
TTTAGAC	90	7.4668587E-6	11.609887	3
>>END_MODULE