FastQCFastQC Report
Thu 2 Feb 2017
SRR4062183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324816
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15960.1204695595463823No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14270.10771307109817514No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13610.10273124720715933No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13510.10197642540549028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC407.035559E-427.4979483
GTATCAA18050.026.4627591
GGTATCA8300.025.4592571
TTATACG550.004485770619.9985084
TAGGTTG1553.45608E-1119.8694865
GTATAGT1352.581146E-919.565911
TTACACG708.127963E-418.8557364
GGCGTTA4000.018.70213542
TATACTG1901.8189894E-1218.5249335
ATCAACG25800.018.5024953
TCAACGC26200.018.2200154
CAACGCA26600.017.946035
CGCAATA2700.017.92661736
TATCAAC27300.017.8095382
AACGCAG27000.017.6801626
TCTATAC1002.4848974E-517.5986883
TCTAGCG2900.017.4489528
GTATAGC1802.8376235E-1017.1201731
ATACGAA3000.016.86859140
CGAGCCG4700.016.84980815
Produced by FastQC (version 0.11.5)