##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062183_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1324816
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.287747883479668	32.0	32.0	32.0	32.0	32.0
2	31.44761536696417	32.0	32.0	32.0	32.0	32.0
3	31.577163923141025	32.0	32.0	32.0	32.0	32.0
4	31.659140590089493	32.0	32.0	32.0	32.0	32.0
5	31.62915151990918	32.0	32.0	32.0	32.0	32.0
6	35.279207829615586	36.0	36.0	36.0	36.0	36.0
7	35.28122244900424	36.0	36.0	36.0	36.0	36.0
8	35.23283308776464	36.0	36.0	36.0	36.0	36.0
9	35.32198358111617	36.0	36.0	36.0	36.0	36.0
10	35.19882836559945	36.0	36.0	36.0	36.0	36.0
11	35.32723638603399	36.0	36.0	36.0	36.0	36.0
12	35.241327097498825	36.0	36.0	36.0	36.0	36.0
13	35.279798100264486	36.0	36.0	36.0	36.0	36.0
14	35.24574657914759	36.0	36.0	36.0	36.0	36.0
15	35.22200214973249	36.0	36.0	36.0	36.0	36.0
16	35.23134533399355	36.0	36.0	36.0	36.0	36.0
17	35.209209429837806	36.0	36.0	36.0	36.0	36.0
18	35.20684155384597	36.0	36.0	36.0	36.0	36.0
19	35.193215510682236	36.0	36.0	36.0	36.0	36.0
20	35.19936806318764	36.0	36.0	36.0	36.0	36.0
21	35.19291131749617	36.0	36.0	36.0	36.0	36.0
22	35.17629617999783	36.0	36.0	36.0	36.0	36.0
23	35.14653280153621	36.0	36.0	36.0	36.0	36.0
24	35.1326410610983	36.0	36.0	36.0	36.0	36.0
25	35.1161821717129	36.0	36.0	36.0	36.0	36.0
26	35.076068676706804	36.0	36.0	36.0	36.0	36.0
27	35.05993964444874	36.0	36.0	36.0	36.0	36.0
28	35.041291017016704	36.0	36.0	36.0	36.0	36.0
29	35.01597353896692	36.0	36.0	36.0	36.0	36.0
30	35.00219200251205	36.0	36.0	36.0	36.0	36.0
31	35.00559171990677	36.0	36.0	36.0	36.0	36.0
32	34.98228508713663	36.0	36.0	36.0	36.0	36.0
33	34.95675625898238	36.0	36.0	36.0	36.0	36.0
34	34.94990021255782	36.0	36.0	36.0	36.0	36.0
35	34.93810914119395	36.0	36.0	36.0	36.0	36.0
36	34.91092574365044	36.0	36.0	36.0	32.0	36.0
37	34.90880167510054	36.0	36.0	36.0	36.0	36.0
38	34.88220099998792	36.0	36.0	36.0	32.0	36.0
39	34.861872894047174	36.0	36.0	36.0	32.0	36.0
40	34.85690541177039	36.0	36.0	36.0	32.0	36.0
41	34.82908645426988	36.0	36.0	36.0	32.0	36.0
42	34.7974375309477	36.0	36.0	36.0	32.0	36.0
43	34.79800138283354	36.0	36.0	36.0	32.0	36.0
44	34.750654430502045	36.0	36.0	36.0	32.0	36.0
45	34.73829648796512	36.0	36.0	36.0	32.0	36.0
46	34.70847272375938	36.0	36.0	36.0	32.0	36.0
47	34.696968484680134	36.0	36.0	36.0	32.0	36.0
48	34.66597399186	36.0	36.0	36.0	32.0	36.0
49	34.65570086713929	36.0	36.0	36.0	32.0	36.0
50	34.2426752092366	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	7.0
21	19.0
22	90.0
23	245.0
24	607.0
25	1364.0
26	2811.0
27	5354.0
28	9482.0
29	15507.0
30	23649.0
31	35681.0
32	54240.0
33	94183.0
34	218014.0
35	863562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.247333222083874	17.71033509998482	11.906058137155483	26.13627354077582
2	16.291717965305388	19.553594276392914	37.20850358442554	26.946184173876155
3	18.862237707869273	23.102842594151166	28.289620179784137	29.745299518195427
4	12.618214618644869	15.715703702026321	35.138868445783	36.527213233545815
5	14.68143500682359	36.553227014166495	33.099011485368536	15.66632649364138
6	35.397572791350335	34.8809719704049	16.15698505601541	13.564470182229355
7	31.02294960205795	29.91743759133344	19.954922042004323	19.104690764604292
8	28.3309531285854	33.31111641163754	18.620774507554255	19.7371559522228
9	26.971594545959586	14.227636139660149	18.455921426069732	40.344847888310525
10	16.54693907743961	26.5989237726381	30.73369600086956	26.120441149052738
11	37.60431637299067	21.145804398497603	21.643911305419017	19.605967923092717
12	24.630967621163997	23.43095192087052	28.375336650523547	23.562743807441937
13	29.585391480779215	19.253239695172763	25.053667830098668	26.10770099394935
14	23.577764761295153	19.42118754604413	24.763740776077583	32.23730691658313
15	25.116469003997537	27.15094020603616	21.817595801983067	25.914994987983235
16	25.940024426217878	25.892168362764057	23.246799908816836	24.92100730220123
17	24.198001835726622	26.20990386589534	24.697014528810037	24.895079769568003
18	24.995093643475453	25.06061237368019	25.775581742918995	24.168712239925362
19	25.75753916015507	24.943992222316155	25.10107063924349	24.197397978285288
20	25.91137459503688	24.279671379788226	24.78283711198268	25.026116913192208
21	26.62415007065132	24.13701223415176	24.503402736681924	24.735434958514993
22	26.073658530694072	24.216796898588182	24.874624098742768	24.834920471974975
23	24.504628948192767	24.25938715971664	25.54839732339987	25.687586568690723
24	24.909118083086	24.718900117452044	25.232715074622806	25.139266724839143
25	25.121545639340255	24.335244800956513	25.196952621921163	25.34625693778207
26	24.735413891100954	25.280097825717746	25.474013156653243	24.510475126528057
27	25.309440599029294	24.820087408760504	24.900325329669915	24.970146662540287
28	24.865903275236075	24.505317823957004	25.474829973052334	25.153948927754588
29	24.71307098025747	24.805839349942065	25.399436143432432	25.08165352636803
30	24.808762720845657	24.903796363812035	25.44493709965474	24.84250381568757
31	25.328234755973288	24.724300920733025	24.669274328320807	25.278189994972877
32	25.266096166750827	24.624189407783646	24.616263701093672	25.49345072437185
33	24.62332306015433	24.582562467495315	25.182648970530845	25.61146550181951
34	25.44134963768116	24.643870772946862	25.230902777777775	24.683876811594203
35	25.72663030432951	24.41286097520105	25.425229503915265	24.43527921655417
36	24.648327460801987	25.05374347454582	24.932141315145092	25.365787749507103
37	25.674954502326024	24.756924289304063	24.839729678523362	24.72839152984655
38	24.890097945676985	24.55450417265492	25.263432808782504	25.291965072885592
39	25.623316266722224	24.481340329058753	24.700843668785463	25.194499735433563
40	25.735064895853384	24.668651849503515	25.00311364228213	24.59316961236097
41	24.655985289832866	25.204219813133776	25.379944716100116	24.759850180933242
42	25.87107001321004	24.97354217776939	24.938818645027364	24.216569163993206
43	25.301413996237798	24.295643877061135	25.196715470622514	25.206226656078552
44	24.825004585774362	24.832855129537744	24.920267914903125	25.421872369784765
45	25.003094844140627	25.099412432517298	24.88677399485501	25.010718728487056
46	24.76624614750553	24.936159741423364	25.043120009118418	25.254474101952685
47	25.12541496767064	24.735620870137577	25.25426441083271	24.884699751359072
48	25.749815626842317	25.31849189173276	24.257091289811957	24.674601191612965
49	25.072805310522767	25.348552121657715	24.432163868939874	25.146478698879648
50	25.211127482653882	25.46346898230062	24.440214010954172	24.885189524091327
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	121.0
1	80.0
2	39.0
3	117.0
4	195.0
5	198.5
6	202.0
7	158.0
8	114.0
9	170.0
10	226.0
11	400.0
12	574.0
13	1102.5
14	1631.0
15	2118.0
16	2605.0
17	2583.0
18	2561.0
19	2500.5
20	2440.0
21	2888.0
22	3336.0
23	4050.5
24	4765.0
25	6133.0
26	7501.0
27	9280.5
28	11060.0
29	13302.5
30	15545.0
31	17986.5
32	20428.0
33	23561.5
34	26695.0
35	30364.5
36	34034.0
37	39986.0
38	45938.0
39	50707.0
40	55476.0
41	60813.5
42	66151.0
43	69132.0
44	72113.0
45	78644.5
46	85176.0
47	91305.0
48	97434.0
49	101419.5
50	105405.0
51	103096.0
52	100787.0
53	99961.0
54	99135.0
55	99876.5
56	100618.0
57	96897.5
58	93177.0
59	85832.0
60	78487.0
61	68484.0
62	58481.0
63	50249.5
64	42018.0
65	35247.0
66	28476.0
67	24546.0
68	20616.0
69	18182.0
70	15748.0
71	12298.0
72	8848.0
73	7805.0
74	6762.0
75	4960.0
76	3158.0
77	2839.5
78	2521.0
79	2146.0
80	1771.0
81	1391.5
82	1012.0
83	865.5
84	719.0
85	563.0
86	407.0
87	286.5
88	166.0
89	110.0
90	54.0
91	45.5
92	37.0
93	29.0
94	21.0
95	20.0
96	19.0
97	13.5
98	8.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03509921377761138
2	0.004302484269513653
3	3.77410900834531E-4
4	7.54821801669062E-5
5	0.0
6	1.509643603338124E-4
7	0.0
8	0.0
9	0.0
10	2.264465405007186E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.54821801669062E-4
17	0.0
18	3.019287206676248E-4
19	0.0
20	3.019287206676248E-4
21	0.0
22	0.0
23	7.54821801669062E-5
24	0.001509643603338124
25	3.77410900834531E-4
26	8.303039818359681E-4
27	4.528930810014372E-4
28	0.001962536684339561
29	8.303039818359681E-4
30	0.0016606079636719362
31	1.509643603338124E-4
32	9.812683421697806E-4
33	5.283752611683434E-4
34	0.0012077148826704991
35	1.509643603338124E-4
36	3.019287206676248E-4
37	0.0017360901438388426
38	0.0
39	5.283752611683434E-4
40	7.54821801669062E-5
41	0.0013586792430043116
42	0.0049818238910158096
43	0.004528930810014372
44	0.005057306071182715
45	0.0024154297653409982
46	0.0018870545041726548
47	0.0010567505223366869
48	0.004302484269513653
49	0.0037741090083453096
50	0.0018115723240057489
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1324816.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.346418764676265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.8952026222655	42.005276665618624
2	14.344940274128785	15.878821431324031
3	4.46856476166011	7.419571697277487
4	1.8020935832476808	3.989577044462485
5	0.9589176002815704	2.6536327533001116
6	0.5725857366547288	1.9014341975743958
7	0.3773401554937804	1.4619098383880778
8	0.27349795858927706	1.210970603789297
9	0.18143947003862002	0.9037822400278556
>10	0.9879611650180367	9.55678303809717
>50	0.07112780220573088	2.7162521131957593
>100	0.058226617330056074	6.649938417219764
>500	0.006454337204244833	2.493274648882708
>1k	0.0016479158819348511	1.1587753108421464
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1596	0.1204695595463823	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1427	0.10771307109817514	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1361	0.10273124720715933	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1351	0.10197642540549028	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.77410900834531E-4
2	0.0	0.0	0.0	0.0	3.77410900834531E-4
3	0.0	0.0	0.0	0.0	3.77410900834531E-4
4	0.0	0.0	0.0	0.0	3.77410900834531E-4
5	0.0	0.0	0.0	0.0	3.77410900834531E-4
6	0.0	0.0	0.0	0.0	3.77410900834531E-4
7	0.0	0.0	0.0	0.0	3.77410900834531E-4
8	0.0	0.0	0.0	0.0	3.77410900834531E-4
9	0.0	0.0	0.0	0.0	3.77410900834531E-4
10	0.0	0.0	0.0	0.0	3.77410900834531E-4
11	0.0	0.0	0.0	0.0	3.77410900834531E-4
12	0.0	0.0	0.0	0.0	9.057861620028743E-4
13	0.0	1.509643603338124E-4	0.0	0.0	0.0010567505223366867
14	0.0	1.509643603338124E-4	0.0	0.0	0.0010567505223366867
15	0.0	1.509643603338124E-4	0.0	0.0	0.0010567505223366867
16	0.0	1.509643603338124E-4	0.0	7.54821801669062E-5	0.0012077148826704991
17	0.0	1.509643603338124E-4	0.0	7.54821801669062E-5	0.0013586792430043116
18	0.0	1.509643603338124E-4	0.0	7.54821801669062E-5	0.0016606079636719362
19	0.0	1.509643603338124E-4	0.0	7.54821801669062E-5	0.0016606079636719362
20	0.0	1.509643603338124E-4	0.0	7.54821801669062E-5	0.001887054504172655
21	0.0	1.509643603338124E-4	0.0	7.54821801669062E-5	0.001962536684339561
22	0.0	1.509643603338124E-4	0.0	1.509643603338124E-4	0.0021135010446733733
23	0.0	1.509643603338124E-4	0.0	2.2644654050071858E-4	0.0021135010446733733
24	0.0	1.509643603338124E-4	0.0	3.77410900834531E-4	0.0021135010446733733
25	0.0	1.509643603338124E-4	0.0	7.54821801669062E-4	0.0021135010446733733
26	0.0	1.509643603338124E-4	0.0	0.0010567505223366867	0.0021135010446733733
27	0.0	1.509643603338124E-4	0.0	0.001509643603338124	0.0024154297653409982
28	0.0	1.509643603338124E-4	0.0	0.0021135010446733733	0.0024154297653409982
29	0.0	1.509643603338124E-4	0.0	0.003094769386843154	0.0024909119455079043
30	0.0	1.509643603338124E-4	0.0	0.004000555548846029	0.0024909119455079043
31	0.0	1.509643603338124E-4	0.0	0.00754821801669062	0.0024909119455079043
32	0.0	1.509643603338124E-4	0.0	0.012077148826704992	0.002566394125674811
33	0.0	1.509643603338124E-4	0.0	0.01547384693421577	0.002566394125674811
34	0.0	1.509643603338124E-4	0.0	0.02045567082523158	0.002943805026509342
35	0.0	2.2644654050071858E-4	0.0	0.024380744193910703	0.002943805026509342
36	0.0	2.2644654050071858E-4	0.0	0.032079926570935134	0.002943805026509342
37	0.0	2.2644654050071858E-4	0.0	0.04219453871330056	0.002943805026509342
38	0.0	2.2644654050071858E-4	0.0	0.054875544981340806	0.0032457337471769663
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAC	40	7.035559E-4	27.497948	3
GTATCAA	1805	0.0	26.462759	1
GGTATCA	830	0.0	25.459257	1
TTATACG	55	0.0044857706	19.998508	4
TAGGTTG	155	3.45608E-11	19.869486	5
GTATAGT	135	2.581146E-9	19.56591	1
TTACACG	70	8.127963E-4	18.855736	4
GGCGTTA	400	0.0	18.702135	42
TATACTG	190	1.8189894E-12	18.524933	5
ATCAACG	2580	0.0	18.502495	3
TCAACGC	2620	0.0	18.220015	4
CAACGCA	2660	0.0	17.94603	5
CGCAATA	270	0.0	17.926617	36
TATCAAC	2730	0.0	17.809538	2
AACGCAG	2700	0.0	17.680162	6
TCTATAC	100	2.4848974E-5	17.598688	3
TCTAGCG	290	0.0	17.44895	28
GTATAGC	180	2.8376235E-10	17.120173	1
ATACGAA	300	0.0	16.868591	40
CGAGCCG	470	0.0	16.849808	15
>>END_MODULE