FastQCFastQC Report
Thu 2 Feb 2017
SRR4062182_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062182_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931646
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17740.19041567290580327No Hit
TATCAACGCAGAGTACTTTTTTTTT12390.13299042769463937No Hit
GGTATCAACGCAGAGTACTTTTTTT9410.1010040294274864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4900.014.3428831
GTATAAT551.963527E-413.8142751
GTACCGT500.00149649213.3026726
TATACCG500.00149649213.3026725
CATTTAG500.00150195613.296241
TTCTATA604.091625E-412.6658082
GTGCTAG751.4813748E-512.6630861
GTCCTAT1800.012.6630851
CGCCAGT2050.012.5093318
TGAGTTA853.934405E-612.29526412
ACCGTGC701.088498E-412.2160838
GTATCAA12000.012.1090761
AGTCGTG550.003062245712.0933377
GCATCGC2050.012.04731614
TCGCCAG2150.011.92685917
CGCATCG2100.011.761106513
TTAGGCT600.00586388111.085564
AACCGCG600.00586388111.085567
CTAGGCT600.00586388111.085564
CGGAGCT600.00586388111.085564
Produced by FastQC (version 0.11.5)