##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062182_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	931646
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26954336733051	32.0	32.0	32.0	32.0	32.0
2	30.83517129896978	32.0	32.0	32.0	32.0	32.0
3	30.85802869330196	32.0	32.0	32.0	32.0	32.0
4	30.86377014445401	32.0	32.0	32.0	32.0	32.0
5	30.77225684433787	32.0	32.0	32.0	32.0	32.0
6	34.43657784179828	36.0	36.0	36.0	32.0	36.0
7	34.378320735558354	36.0	36.0	36.0	32.0	36.0
8	34.33980503324224	36.0	36.0	36.0	32.0	36.0
9	34.46262206889742	36.0	36.0	36.0	32.0	36.0
10	34.15487212954277	36.0	36.0	36.0	32.0	36.0
11	34.44423525673915	36.0	36.0	36.0	32.0	36.0
12	34.26760056931496	36.0	36.0	36.0	32.0	36.0
13	34.3611843983659	36.0	36.0	36.0	32.0	36.0
14	34.256604976568354	36.0	36.0	36.0	32.0	36.0
15	34.214085607623495	36.0	36.0	36.0	32.0	36.0
16	34.21349525463535	36.0	36.0	36.0	32.0	36.0
17	34.14787376321049	36.0	36.0	36.0	32.0	36.0
18	34.16115992555112	36.0	36.0	36.0	32.0	36.0
19	34.16215493867843	36.0	36.0	36.0	32.0	36.0
20	34.14730595097279	36.0	36.0	36.0	32.0	36.0
21	34.12731981890117	36.0	36.0	36.0	32.0	36.0
22	34.08998052908508	36.0	36.0	36.0	32.0	36.0
23	34.061428911839904	36.0	36.0	36.0	32.0	36.0
24	34.04461887884454	36.0	36.0	36.0	32.0	36.0
25	33.698471307771406	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	14.0
5	82.0
6	193.0
7	44.0
8	151.0
9	122.0
10	73.0
11	16.0
12	37.0
13	41.0
14	155.0
15	219.0
16	360.0
17	460.0
18	656.0
19	870.0
20	1210.0
21	1729.0
22	2739.0
23	3958.0
24	5591.0
25	7716.0
26	10603.0
27	13473.0
28	17664.0
29	23454.0
30	30997.0
31	42072.0
32	59809.0
33	85508.0
34	190163.0
35	431467.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.780387274054355	17.834571293632735	12.120454252715641	25.26458717959727
2	16.69294634758948	19.185097327844833	37.58910314551143	26.532853179054257
3	19.32390121066207	22.591475882825858	28.381916171392568	29.702706735119506
4	13.060416085192262	15.631749575970973	35.13311290980527	36.1747214290315
5	15.268818127936642	35.991307111504334	33.12273980604207	15.61713495451695
6	34.89384188412397	34.670226751329665	16.453863536770836	13.982067827775534
7	30.97550623492309	30.1248059141778	19.67989942459262	19.219788426306486
8	28.390282165345027	33.06932714417725	18.819439967335008	19.720950723142717
9	26.82394762746034	14.819292386122823	18.975696459351532	39.38106352706531
10	17.012719720133916	26.74945859239396	30.368479598471698	25.869342089000426
11	36.947350431392614	21.54587637675349	21.603058604259214	19.90371458759468
12	24.422545111396047	23.676429651587082	28.366467150821293	23.534558086195574
13	29.32933244918379	19.48926392401186	25.011042913136606	26.17036071366774
14	23.49542982039506	19.704319158218695	25.032183606670124	31.76806741471613
15	25.43074957035209	27.009510830136723	21.956163553174772	25.603576046336414
16	25.802451225344015	26.092493189548172	23.375834851244687	24.729220733863126
17	24.107187955057146	26.194634154204934	24.850217352756268	24.847960537981656
18	24.740680565092322	25.391974502718114	25.640951248053696	24.22639368413587
19	25.651574113667962	25.232985153844332	25.226001508467398	23.8894392240203
20	25.80631298942535	24.625514739297234	24.895494359220077	24.672677912057335
21	26.6568471987658	24.31897585313342	24.53706687981573	24.48711006828505
22	25.928850867042087	24.397411391296593	24.98256675402093	24.691170987640394
23	24.68333965347029	24.450013804038516	25.289428254986912	25.577218287504284
24	24.51324495840706	25.07748652513357	25.349615978392944	25.05965253806643
25	24.94021387639689	24.69677208648737	25.177746884997926	25.185267152117813
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	364.0
1	364.0
2	364.0
3	364.0
4	364.0
5	364.0
6	667.0
7	970.0
8	970.0
9	970.0
10	1247.5
11	1525.0
12	1525.0
13	1525.0
14	2080.5
15	2636.0
16	2636.0
17	2636.0
18	4404.0
19	6172.0
20	6172.0
21	6172.0
22	9521.5
23	12871.0
24	12871.0
25	12871.0
26	18861.0
27	24851.0
28	24851.0
29	24851.0
30	30974.5
31	37098.0
32	37098.0
33	37098.0
34	46047.0
35	54996.0
36	54996.0
37	54996.0
38	64802.5
39	74609.0
40	74609.0
41	74609.0
42	86740.5
43	98872.0
44	98872.0
45	98872.0
46	109757.0
47	120642.0
48	120642.0
49	120642.0
50	124746.5
51	128851.0
52	128851.0
53	128851.0
54	120206.0
55	111561.0
56	111561.0
57	111561.0
58	102620.5
59	93680.0
60	93680.0
61	93680.0
62	82457.0
63	71234.0
64	71234.0
65	71234.0
66	57868.0
67	44502.0
68	44502.0
69	44502.0
70	33667.0
71	22832.0
72	22832.0
73	22832.0
74	17130.5
75	11429.0
76	11429.0
77	11429.0
78	9145.0
79	6861.0
80	6861.0
81	6861.0
82	4717.5
83	2574.0
84	2574.0
85	2574.0
86	1915.0
87	1256.0
88	1256.0
89	1256.0
90	844.0
91	432.0
92	432.0
93	432.0
94	272.5
95	113.0
96	113.0
97	113.0
98	232.0
99	351.0
100	351.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.440214416205297E-4
2	1.0733690693675495E-4
3	7.513583485572846E-4
4	0.011377712135296024
5	0.03327444115039403
6	0.062470079837191377
7	0.10744424384369171
8	0.10583419023964039
9	0.11613853330556885
10	0.1289116252310427
11	0.13814259922760364
12	0.13170238481139832
13	0.12719423472005462
14	0.11248907846971919
15	0.13234640625301886
16	0.11710456546799965
17	0.12139804174546985
18	0.1113083724934149
19	0.09660321624307945
20	0.08962631729219038
21	0.09037767564074767
22	0.10229207231072747
23	0.081468712364997
24	0.0900556649199374
25	0.0888749589436331
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	931646.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.012383725747085
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.16557607808457	45.925837773116655
2	12.926529533851848	14.997975831200325
3	3.606687557894169	6.276976277623027
4	1.4241245512131058	3.3046743975292823
5	0.7059805346304922	2.0477806838946093
6	0.458273910669594	1.5951337174357938
7	0.30629433253060584	1.2438205046250959
8	0.2154012516319074	0.9996752051741135
9	0.14849478044027858	0.7753082565754804
>10	0.8828460019361878	9.425347396467957
>50	0.08722981794987336	3.6155439580847366
>100	0.06736541862631354	7.564995741157106
>500	0.004825071216825591	1.9025531304689438
>1k	3.711593243711993E-4	0.32437712664683843
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1774	0.19041567290580327	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1239	0.13299042769463937	No Hit
GGTATCAACGCAGAGTACTTTTTTT	941	0.1010040294274864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0733690693675495E-4
2	0.0	0.0	0.0	0.0	1.0733690693675495E-4
3	0.0	0.0	0.0	0.0	1.0733690693675495E-4
4	0.0	0.0	0.0	0.0	1.0733690693675495E-4
5	0.0	0.0	0.0	0.0	1.0733690693675495E-4
6	0.0	0.0	0.0	0.0	1.0733690693675495E-4
7	0.0	0.0	0.0	1.0733690693675495E-4	1.0733690693675495E-4
8	0.0	0.0	0.0	1.0733690693675495E-4	1.0733690693675495E-4
9	0.0	0.0	0.0	1.0733690693675495E-4	1.0733690693675495E-4
10	0.0	0.0	0.0	1.0733690693675495E-4	1.0733690693675495E-4
11	0.0	0.0	0.0	1.0733690693675495E-4	1.0733690693675495E-4
12	0.0	0.0	0.0	1.0733690693675495E-4	3.2201072081026484E-4
13	0.0	0.0	0.0	1.0733690693675495E-4	4.293476277470198E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	490	0.0	14.342883	1
GTATAAT	55	1.963527E-4	13.814275	1
GTACCGT	50	0.001496492	13.302672	6
TATACCG	50	0.001496492	13.302672	5
CATTTAG	50	0.001501956	13.29624	1
TTCTATA	60	4.091625E-4	12.665808	2
GTGCTAG	75	1.4813748E-5	12.663086	1
GTCCTAT	180	0.0	12.663085	1
CGCCAGT	205	0.0	12.50933	18
TGAGTTA	85	3.934405E-6	12.295264	12
ACCGTGC	70	1.088498E-4	12.216083	8
GTATCAA	1200	0.0	12.109076	1
AGTCGTG	55	0.0030622457	12.093337	7
GCATCGC	205	0.0	12.047316	14
TCGCCAG	215	0.0	11.926859	17
CGCATCG	210	0.0	11.7611065	13
TTAGGCT	60	0.005863881	11.08556	4
AACCGCG	60	0.005863881	11.08556	7
CTAGGCT	60	0.005863881	11.08556	4
CGGAGCT	60	0.005863881	11.08556	4
>>END_MODULE