FastQCFastQC Report
Thu 2 Feb 2017
SRR4062182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931646
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9410.1010040294274864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13400.024.960431
GGTATCA7650.022.1484131
TGTAGGA1800.019.5543882
TAAGGTT951.5972908E-518.525215
CGCAATA2150.018.41948136
CGGACTT600.007408823418.33420626
ACGGACC3850.018.2846228
TCTAGCG2200.018.00085628
CTAGCGG2250.017.60083829
CGTCTTA750.00129108617.5989515
AAATTCG1154.2731917E-617.21636421
ACACGCT2450.017.0602059
CGGATCG1307.3087176E-716.92388226
CGGACCA3550.016.7313949
AGCGAAA3950.016.7078617
TACGAAT2400.016.50255641
TCAACGC20200.016.4445614
CCTATTC2151.2732926E-1116.3711153
ATCGATT1351.0842505E-616.29707129
GCGAAAG3650.016.27318
Produced by FastQC (version 0.11.5)