##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062182_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	931646
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.309497384199577	32.0	32.0	32.0	32.0	32.0
2	31.488703863913976	32.0	32.0	32.0	32.0	32.0
3	31.602685998759185	32.0	32.0	32.0	32.0	32.0
4	31.67126354860108	32.0	32.0	32.0	32.0	32.0
5	31.649667362925403	32.0	32.0	32.0	32.0	32.0
6	35.30552377190478	36.0	36.0	36.0	36.0	36.0
7	35.30455666637328	36.0	36.0	36.0	36.0	36.0
8	35.26315682136777	36.0	36.0	36.0	36.0	36.0
9	35.34208808925278	36.0	36.0	36.0	36.0	36.0
10	35.2300251383036	36.0	36.0	36.0	36.0	36.0
11	35.338307683390475	36.0	36.0	36.0	36.0	36.0
12	35.26845497109417	36.0	36.0	36.0	36.0	36.0
13	35.29492639908291	36.0	36.0	36.0	36.0	36.0
14	35.263106373021515	36.0	36.0	36.0	36.0	36.0
15	35.24374279501012	36.0	36.0	36.0	36.0	36.0
16	35.26276182155024	36.0	36.0	36.0	36.0	36.0
17	35.243450838623254	36.0	36.0	36.0	36.0	36.0
18	35.22991458128946	36.0	36.0	36.0	36.0	36.0
19	35.22261030477242	36.0	36.0	36.0	36.0	36.0
20	35.228950695865166	36.0	36.0	36.0	36.0	36.0
21	35.21849930123673	36.0	36.0	36.0	36.0	36.0
22	35.20639491824148	36.0	36.0	36.0	36.0	36.0
23	35.17378596591409	36.0	36.0	36.0	36.0	36.0
24	35.16081752081799	36.0	36.0	36.0	36.0	36.0
25	35.14170833127604	36.0	36.0	36.0	36.0	36.0
26	35.10558087513927	36.0	36.0	36.0	36.0	36.0
27	35.09065997170599	36.0	36.0	36.0	36.0	36.0
28	35.07691977424902	36.0	36.0	36.0	36.0	36.0
29	35.055109988128535	36.0	36.0	36.0	36.0	36.0
30	35.03205080041131	36.0	36.0	36.0	36.0	36.0
31	35.03682514603186	36.0	36.0	36.0	36.0	36.0
32	35.009769805269386	36.0	36.0	36.0	36.0	36.0
33	34.99361667414447	36.0	36.0	36.0	36.0	36.0
34	34.97335146611481	36.0	36.0	36.0	36.0	36.0
35	34.97173926577262	36.0	36.0	36.0	36.0	36.0
36	34.94289354540244	36.0	36.0	36.0	36.0	36.0
37	34.93119811602261	36.0	36.0	36.0	36.0	36.0
38	34.913978056042744	36.0	36.0	36.0	36.0	36.0
39	34.90464511198459	36.0	36.0	36.0	36.0	36.0
40	34.880189471108125	36.0	36.0	36.0	36.0	36.0
41	34.862679601479535	36.0	36.0	36.0	32.0	36.0
42	34.83235370516269	36.0	36.0	36.0	32.0	36.0
43	34.829003720297194	36.0	36.0	36.0	32.0	36.0
44	34.781132533172475	36.0	36.0	36.0	32.0	36.0
45	34.768429210236505	36.0	36.0	36.0	32.0	36.0
46	34.74629848676429	36.0	36.0	36.0	32.0	36.0
47	34.7194921676259	36.0	36.0	36.0	32.0	36.0
48	34.690858974331455	36.0	36.0	36.0	32.0	36.0
49	34.67739248598717	36.0	36.0	36.0	32.0	36.0
50	34.27963733005884	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	10.0
21	31.0
22	76.0
23	172.0
24	408.0
25	998.0
26	2074.0
27	3942.0
28	6802.0
29	10797.0
30	16041.0
31	24087.0
32	36343.0
33	62465.0
34	145950.0
35	621449.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.500878832746906	18.345969126503597	12.589158306928983	25.56399373382051
2	16.165393760164022	19.605193243845235	37.35078011367601	26.87863288231474
3	18.586431268663283	22.97309481539046	28.68086668484246	29.75960723110379
4	12.792090558001645	16.036348570165064	35.3363831326491	35.835177739184196
5	14.829023040940442	36.417480459316096	32.98044536229426	15.773051137449205
6	34.96671482515891	34.853259714526764	16.372742436504854	13.807283023809472
7	31.007378338982832	30.156304003881303	19.69041889301301	19.145898764122855
8	28.44857381451753	33.25275909519281	18.680163924924273	19.618503165365386
9	26.957986187886817	14.50164547478334	18.87680513843241	39.663563198897435
10	17.10220405604704	26.66688849627434	30.365288961687163	25.865618485991458
11	37.20050319541972	21.451602862031287	21.556578357015432	19.79131558553356
12	24.40057704321169	23.72553523548644	28.361416246084886	23.51247147521698
13	29.591926547207848	19.465655409887447	25.05060935162068	25.89180869128403
14	23.60853800692538	19.482292630462645	25.11189872548157	31.797270637130413
15	25.32807525605219	27.04535843013333	21.978197727463005	25.648368586351467
16	25.768942195297097	26.04168791063472	23.4506572838992	24.73871261016898
17	24.212200771537688	26.14458710712009	24.88058769103286	24.762624430309366
18	24.958648412217098	25.209280358935004	25.763998089401007	24.06807313944689
19	25.62768007127178	25.161515384078697	25.358800830788553	23.852003713860967
20	25.855182725571918	24.55489924788787	24.878306389893982	24.71161163664623
21	26.650787960233824	24.272309439422273	24.670958711785378	24.40594388855853
22	26.02061297960813	24.259214336775987	25.15816093237131	24.562011751244572
23	24.734958058058595	24.23680693826511	25.452613388146773	25.57562161552952
24	24.69311917151837	24.85691775293249	25.39629381558384	25.053669259965304
25	25.107202850871307	24.48561415560648	25.410000590353622	24.997182403168587
26	24.738150491283132	25.303603744786084	25.560676740636424	24.397569023294356
27	25.282940694084864	24.897600370957147	25.08855313832322	24.730905796634765
28	24.816021776838923	24.711688735466364	25.603135143490135	24.869154344204578
29	24.78830364915241	24.923441540249947	25.52152599552832	24.766728815069325
30	24.696956848887922	24.9063740764872	25.69176918378804	24.704899890836842
31	25.192536610132017	24.817177432079525	24.824047030991554	25.166238926796908
32	25.14090788579672	24.850773423554454	24.753310570533372	25.255008120115452
33	24.727202079768837	24.677504878483365	25.258307375579893	25.336985666167905
34	25.291368535847614	24.69274546551343	25.533200663777134	24.482685334861824
35	25.779374954381716	24.396013928066946	25.627278230740497	24.19733288681084
36	24.671199866902093	25.048704173800107	25.19800997160936	25.082085987688448
37	25.62600093599591	24.873125324700418	24.896417883532912	24.60445585577076
38	24.865667860968653	24.59839896269613	25.372512735524005	25.16342044081121
39	25.48591939341116	24.600809758317627	24.97649296506361	24.936777883207604
40	25.761422000869427	24.67141454094639	25.100011270387323	24.467152187796852
41	24.611276053574457	25.183064666748244	25.475452911967956	24.730206367709346
42	25.662114278783037	25.000778231835803	25.224157604291115	24.112949885090043
43	25.416325855891248	24.270664940607684	25.230302210600662	25.0827069929004
44	24.695707432585472	24.98145637498162	25.237578454830306	25.085257737602607
45	25.0892260066572	25.034053474420446	25.119495761697596	24.75722475722476
46	24.929504443340033	25.09029978993558	24.945927466056403	25.03426830066798
47	25.08428730135729	24.920059894701254	25.24497254826193	24.75068025567953
48	25.81737968344953	25.28281836185937	24.319749249950355	24.580052704740744
49	25.055763438505647	25.416427833845347	24.709268001554292	24.818540726094714
50	25.270733710090326	25.366157347029684	24.525484766029614	24.837624176850376
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	122.0
1	75.5
2	29.0
3	85.0
4	141.0
5	151.0
6	161.0
7	121.0
8	81.0
9	134.0
10	187.0
11	314.0
12	441.0
13	852.0
14	1263.0
15	1632.5
16	2002.0
17	2083.5
18	2165.0
19	2085.5
20	2006.0
21	2401.0
22	2796.0
23	3234.0
24	3672.0
25	4655.0
26	5638.0
27	7008.5
28	8379.0
29	9813.5
30	11248.0
31	12800.0
32	14352.0
33	16395.5
34	18439.0
35	21226.5
36	24014.0
37	28321.5
38	32629.0
39	35842.5
40	39056.0
41	42746.0
42	46436.0
43	48681.0
44	50926.0
45	55952.5
46	60979.0
47	65319.5
48	69660.0
49	72490.5
50	75321.0
51	73479.0
52	71637.0
53	70894.0
54	70151.0
55	70936.5
56	71722.0
57	68554.5
58	65387.0
59	59319.5
60	53252.0
61	46287.5
62	39323.0
63	33705.5
64	28088.0
65	23556.5
66	19025.0
67	16263.5
68	13502.0
69	11838.5
70	10175.0
71	8064.0
72	5953.0
73	5368.0
74	4783.0
75	3406.5
76	2030.0
77	1876.0
78	1722.0
79	1479.5
80	1237.0
81	947.5
82	658.0
83	533.5
84	409.0
85	331.5
86	254.0
87	170.5
88	87.0
89	63.5
90	40.0
91	30.5
92	21.0
93	18.0
94	15.0
95	16.5
96	18.0
97	15.0
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03187906136021622
2	0.0054741822537745025
3	4.293476277470198E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.660321624307945E-4
17	0.0
18	1.0733690693675495E-4
19	1.0733690693675495E-4
20	3.2201072081026484E-4
21	0.0
22	0.0
23	1.0733690693675495E-4
24	0.0015027166971145693
25	1.0733690693675495E-4
26	4.293476277470198E-4
27	2.146738138735099E-4
28	0.0015027166971145693
29	5.366845346837747E-4
30	0.0013953797901778144
31	5.366845346837747E-4
32	9.660321624307945E-4
33	4.293476277470198E-4
34	8.586952554940396E-4
35	2.146738138735099E-4
36	1.0733690693675495E-4
37	0.001932064324861589
38	0.0
39	0.0010733690693675495
40	1.0733690693675495E-4
41	8.586952554940396E-4
42	0.005044834626027482
43	0.004722823905217218
44	0.006332877509268541
45	0.002468748859545364
46	0.0028980964872923833
47	0.0011807059763043045
48	0.004400813184406952
49	0.0034347810219761583
50	0.0011807059763043045
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	931646.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.4567742933817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.02126708410135	46.983496413964474
2	13.133170865748989	15.61711951842508
3	3.662721104148227	6.533207459668408
4	1.3915952949168988	3.3095906943042404
5	0.7240767142950136	2.1525632886466024
6	0.4504703398294304	1.6070107992660838
7	0.3024591550659967	1.258827200100813
8	0.20227064281380913	0.9621087964766304
9	0.14980666392718672	0.8016318904286905
>10	0.8206356796463323	8.991148273372993
>50	0.07784337481528474	3.270389497858102
>100	0.05896581557981003	6.674618995333045
>500	0.004717265111770795	1.8382871721548226
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	941	0.1010040294274864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0733690693675495E-4
2	0.0	0.0	0.0	0.0	1.0733690693675495E-4
3	0.0	0.0	0.0	0.0	1.0733690693675495E-4
4	0.0	0.0	0.0	0.0	2.146738138735099E-4
5	0.0	0.0	0.0	0.0	2.146738138735099E-4
6	0.0	0.0	0.0	0.0	2.146738138735099E-4
7	0.0	0.0	0.0	0.0	2.146738138735099E-4
8	0.0	0.0	0.0	0.0	2.146738138735099E-4
9	0.0	0.0	0.0	1.0733690693675495E-4	3.2201072081026484E-4
10	0.0	0.0	0.0	1.0733690693675495E-4	3.2201072081026484E-4
11	0.0	0.0	0.0	1.0733690693675495E-4	3.2201072081026484E-4
12	0.0	0.0	0.0	1.0733690693675495E-4	5.366845346837747E-4
13	0.0	0.0	0.0	1.0733690693675495E-4	7.513583485572846E-4
14	0.0	0.0	0.0	1.0733690693675495E-4	7.513583485572846E-4
15	0.0	0.0	0.0	1.0733690693675495E-4	7.513583485572846E-4
16	0.0	0.0	0.0	2.146738138735099E-4	7.513583485572846E-4
17	0.0	0.0	0.0	2.146738138735099E-4	7.513583485572846E-4
18	0.0	0.0	0.0	3.2201072081026484E-4	8.586952554940396E-4
19	0.0	0.0	0.0	3.2201072081026484E-4	8.586952554940396E-4
20	0.0	0.0	0.0	3.2201072081026484E-4	0.0010733690693675495
21	0.0	0.0	0.0	4.293476277470198E-4	0.0010733690693675495
22	0.0	0.0	0.0	5.366845346837747E-4	0.0011807059763043043
23	0.0	0.0	0.0	6.440214416205297E-4	0.0012880428832410594
24	0.0	0.0	0.0	0.0010733690693675495	0.0012880428832410594
25	0.0	0.0	0.0	0.0013953797901778142	0.0012880428832410594
26	0.0	0.0	0.0	0.0016100536040513241	0.0012880428832410594
27	0.0	0.0	0.0	0.001932064324861589	0.0013953797901778142
28	0.0	0.0	0.0	0.0025760857664821188	0.0013953797901778142
29	0.0	0.0	0.0	0.0037567917427864233	0.0013953797901778142
30	0.0	0.0	0.0	0.006762225137015561	0.0013953797901778142
31	0.0	0.0	0.0	0.011163038321422514	0.0015027166971145693
32	0.0	0.0	0.0	0.015885862226639732	0.0016100536040513241
33	0.0	0.0	0.0	0.02060868613185695	0.0016100536040513241
34	0.0	0.0	0.0	0.02565352075788443	0.0016100536040513241
35	0.0	0.0	0.0	0.032630419708773506	0.0016100536040513241
36	0.0	0.0	0.0	0.0412173722637139	0.0016100536040513241
37	0.0	0.0	0.0	0.05280975821288343	0.0016100536040513241
38	0.0	0.0	0.0	0.06751491446321886	0.0016100536040513241
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1340	0.0	24.96043	1
GGTATCA	765	0.0	22.148413	1
TGTAGGA	180	0.0	19.554388	2
TAAGGTT	95	1.5972908E-5	18.52521	5
CGCAATA	215	0.0	18.419481	36
CGGACTT	60	0.0074088234	18.334206	26
ACGGACC	385	0.0	18.284622	8
TCTAGCG	220	0.0	18.000856	28
CTAGCGG	225	0.0	17.600838	29
CGTCTTA	75	0.001291086	17.59895	15
AAATTCG	115	4.2731917E-6	17.216364	21
ACACGCT	245	0.0	17.060205	9
CGGATCG	130	7.3087176E-7	16.923882	26
CGGACCA	355	0.0	16.731394	9
AGCGAAA	395	0.0	16.70786	17
TACGAAT	240	0.0	16.502556	41
TCAACGC	2020	0.0	16.444561	4
CCTATTC	215	1.2732926E-11	16.371115	3
ATCGATT	135	1.0842505E-6	16.297071	29
GCGAAAG	365	0.0	16.273	18
>>END_MODULE