FastQCFastQC Report
Thu 2 Feb 2017
SRR4062181_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062181_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2443774
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC45910.18786516265415706No Hit
GGGTAGGCACACGCTGAGCCAGTCA41260.16883721653475323No Hit
GAATAGGACCGCGGTTCTATTTTGT36320.14862258130252634No Hit
ATCAGATACCGTCGTAGTTCCGACC36260.14837705941711465No Hit
TCGTAGTTCCGACCATAAACGATGC36020.14739497187546804No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC35610.14571723899182168No Hit
GATTAAGAGGGACGGCCGGGGGCAT35270.14432594830782225No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG34330.1404794387697062No Hit
GTATCAACGCAGAGTACTTTTTTTT33690.13786053865864845No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT32190.1317224915233569No Hit
GTATCTGATCGTCTTCGAACCTCCG31300.12808058355641724No Hit
GTGCATGGCCGTTCTTAGTTGGTGG31200.12767138041406448No Hit
GAACTACGACGGTATCTGATCGTCT30530.12492971936030092No Hit
ACCATACTCCCCCCGGAACCCAAAG30520.12488879904606563No Hit
GTCTTGCGCCGGTCCAAGAATTTCA27950.11437227828759942No Hit
GCCATGCACCACCACCCACGGAATC27570.11281730634665889No Hit
GTACATGGGGAATAATTGCAATCCC26600.10884803586583701No Hit
GAATAACGCCGCCGCATCGCCAGTC26130.106924781096779No Hit
GAGTATGGTTGCAAAGCTGAAACTT25700.10516520758466208No Hit
GTTATTGCTCAATCTCGGGTGGCTG24800.10148237930348715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA2200.014.68254716
GGTATCA11450.014.2650181
GTTCGCG602.5682439E-514.25070716
CGTTCGC801.9991603E-613.06341615
ACGAACG2500.012.92090615
TTCTACG1052.0008883E-812.6641812
CGTTATT3350.012.47516252
GCGTTAT3450.012.3863361
GTATCAA30100.012.3355831
TTCGCGC701.09193075E-412.21364117
AATCGTA550.003067697212.09175713
ATAACGA2800.011.87607612
TAACGAA2800.011.87583313
ACTCTAA4750.011.80179410
GTATTAT1052.736324E-711.7571881
TCGCGCA658.0326176E-411.69121118
AACGAAC2800.011.53652314
CGACCAT6850.011.512710
CGATAAC2900.011.46726110
CTACTAA752.0726E-411.4012679
Produced by FastQC (version 0.11.5)