##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062181_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2443774
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23146657587813	32.0	32.0	32.0	32.0	32.0
2	30.744636779014755	32.0	32.0	32.0	32.0	32.0
3	30.75573600504793	32.0	32.0	32.0	32.0	32.0
4	30.760541686751722	32.0	32.0	32.0	32.0	32.0
5	30.671884552335854	32.0	32.0	32.0	32.0	32.0
6	34.329129862254035	36.0	36.0	36.0	32.0	36.0
7	34.21682160461646	36.0	36.0	36.0	32.0	36.0
8	34.20532913436349	36.0	36.0	36.0	32.0	36.0
9	34.28498911928845	36.0	36.0	36.0	32.0	36.0
10	33.98118729473347	36.0	36.0	36.0	32.0	36.0
11	34.3385922757178	36.0	36.0	36.0	32.0	36.0
12	34.08952382667137	36.0	36.0	36.0	32.0	36.0
13	34.203153810458744	36.0	36.0	36.0	32.0	36.0
14	34.11201444978136	36.0	36.0	36.0	32.0	36.0
15	34.028933526586336	36.0	36.0	36.0	32.0	36.0
16	34.04539167697177	36.0	36.0	36.0	32.0	36.0
17	34.00364968282665	36.0	36.0	36.0	32.0	36.0
18	34.01877219415543	36.0	36.0	36.0	32.0	36.0
19	33.989073457692896	36.0	36.0	36.0	32.0	36.0
20	34.018464882595524	36.0	36.0	36.0	32.0	36.0
21	33.988725225818754	36.0	36.0	36.0	32.0	36.0
22	33.9321414337005	36.0	36.0	36.0	32.0	36.0
23	33.90343665167074	36.0	36.0	36.0	32.0	36.0
24	33.893884213515655	36.0	36.0	36.0	32.0	36.0
25	33.513011023114245	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	37.0
5	195.0
6	508.0
7	116.0
8	417.0
9	334.0
10	182.0
11	56.0
12	125.0
13	100.0
14	332.0
15	472.0
16	767.0
17	1044.0
18	1505.0
19	2148.0
20	3362.0
21	4769.0
22	7541.0
23	11399.0
24	16156.0
25	22696.0
26	31593.0
27	40243.0
28	53022.0
29	68748.0
30	89114.0
31	120914.0
32	168782.0
33	236319.0
34	509337.0
35	1051438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.489774514301196	17.21282931498228	11.56809604267545	25.72930012804108
2	17.495229704607922	19.00249164303173	36.269426447426085	27.232852204934265
3	19.47615107551566	22.212592245222318	27.54785866685654	30.763398012405485
4	13.135092241816427	15.131349285437459	34.021615511701754	37.71194296104436
5	15.900350598555443	35.528507367072386	32.29686057720843	16.274281457163735
6	36.205602509512836	33.95900544871546	15.855487634851306	13.979904406920404
7	32.00105686377722	29.476668093569312	19.39017321505744	19.132101827596024
8	29.1550342979921	32.014108364859354	18.430178136540622	20.40067920060792
9	26.942277083926523	14.45750209452719	18.458141206980255	40.14207961456603
10	17.430105152139326	25.546852176079334	29.17712704343554	27.845915628345804
11	38.070797867196426	20.868594582101956	21.086021875496854	19.974585675204764
12	25.150263198761262	23.381646193830818	27.229875548595377	24.23821505881254
13	29.314119271028165	18.694704633164292	24.967641723922753	27.02353437188479
14	24.258800413616573	19.39126751192589	23.688315987329307	32.66161608712823
15	25.842442956529077	26.588381066406725	21.226641033110965	26.34253494395323
16	26.940222652765993	25.308285136147745	22.41571655015628	25.335775660929983
17	24.792469110562003	25.69179353150155	24.091897435225082	25.423839922711363
18	25.81665635664218	24.26179541907177	25.09014784984781	24.83140037443824
19	26.081218519457572	24.62893049817133	24.298217121482892	24.991633860888207
20	26.437053727035874	23.674907463574215	23.728686517267665	26.15935229212225
21	27.751248300750476	23.5996115532994	23.46072260752425	25.188417538425874
22	26.958310906408244	23.416044652315072	24.21739247979119	25.408251961485494
23	24.97429069554573	23.728612950772877	24.78024739087931	26.516848962802086
24	25.27831433669031	24.483306054842053	24.492276009181303	25.746103599286336
25	25.474135865498393	23.842670241449376	24.296808539831506	26.386385353220724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	132.0
1	132.0
2	378.0
3	624.0
4	624.0
5	624.0
6	1491.0
7	2358.0
8	2358.0
9	2358.0
10	2661.5
11	2965.0
12	2965.0
13	2965.0
14	3980.5
15	4996.0
16	4996.0
17	4996.0
18	8297.5
19	11599.0
20	11599.0
21	11599.0
22	18936.5
23	26274.0
24	26274.0
25	26274.0
26	40178.5
27	54083.0
28	54083.0
29	54083.0
30	67059.5
31	80036.0
32	80036.0
33	80036.0
34	104813.0
35	129590.0
36	129590.0
37	129590.0
38	154121.5
39	178653.0
40	178653.0
41	178653.0
42	209354.0
43	240055.0
44	240055.0
45	240055.0
46	271718.5
47	303382.0
48	303382.0
49	303382.0
50	320106.5
51	336831.0
52	336831.0
53	336831.0
54	317314.0
55	297797.0
56	297797.0
57	297797.0
58	281508.5
59	265220.0
60	265220.0
61	265220.0
62	238766.0
63	212312.0
64	212312.0
65	212312.0
66	176005.0
67	139698.0
68	139698.0
69	139698.0
70	106996.0
71	74294.0
72	74294.0
73	74294.0
74	57429.5
75	40565.0
76	40565.0
77	40565.0
78	32934.0
79	25303.0
80	25303.0
81	25303.0
82	17433.0
83	9563.0
84	9563.0
85	9563.0
86	7079.0
87	4595.0
88	4595.0
89	4595.0
90	3074.0
91	1553.0
92	1553.0
93	1553.0
94	943.0
95	333.0
96	333.0
97	333.0
98	648.0
99	963.0
100	963.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001104848484352481
2	2.0460157117638538E-4
3	6.547250277644332E-4
4	0.010270998873054545
5	0.03310453421633915
6	0.06330372612197364
7	0.10594269355513235
8	0.10921631869395451
9	0.11821878782571546
10	0.1315588102664158
11	0.13986563405617705
12	0.13188617278029802
13	0.12812150387065252
14	0.11727762059830409
15	0.13188617278029802
16	0.12001928165206767
17	0.12492971936030092
18	0.11392215483101138
19	0.09759494945113582
20	0.0942394836838431
21	0.0922753086005498
22	0.10279182935901601
23	0.08392756449655328
24	0.0937075195987845
25	0.09112953980196205
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2443774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.44198099581345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.79322154225724	29.03460101240192
2	14.728179216836685	11.912734879804878
3	5.253525247556778	6.373889046681523
4	2.5164515739787356	4.070811469269315
5	1.4090023246926906	2.8491422619139386
6	0.8763123715077641	2.126388496494793
7	0.5965679433587734	1.6888472599618922
8	0.4333550574773615	1.402058959915128
9	0.32670985895099114	1.1891514516156791
>10	1.798031926836941	13.21630599615497
>50	0.1325876902306466	3.7247535436309613
>100	0.10720425528961898	9.142228855348618
>500	0.017574723406559356	5.008040516121856
>1k	0.011276267619237487	8.261046250684736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	4591	0.18786516265415706	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	4126	0.16883721653475323	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3632	0.14862258130252634	No Hit
ATCAGATACCGTCGTAGTTCCGACC	3626	0.14837705941711465	No Hit
TCGTAGTTCCGACCATAAACGATGC	3602	0.14739497187546804	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	3561	0.14571723899182168	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3527	0.14432594830782225	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3433	0.1404794387697062	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3369	0.13786053865864845	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	3219	0.1317224915233569	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3130	0.12808058355641724	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	3120	0.12767138041406448	No Hit
GAACTACGACGGTATCTGATCGTCT	3053	0.12492971936030092	No Hit
ACCATACTCCCCCCGGAACCCAAAG	3052	0.12488879904606563	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2795	0.11437227828759942	No Hit
GCCATGCACCACCACCCACGGAATC	2757	0.11281730634665889	No Hit
GTACATGGGGAATAATTGCAATCCC	2660	0.10884803586583701	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2613	0.106924781096779	No Hit
GAGTATGGTTGCAAAGCTGAAACTT	2570	0.10516520758466208	No Hit
GTTATTGCTCAATCTCGGGTGGCTG	2480	0.10148237930348715	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.0920314235277076E-5
2	0.0	0.0	0.0	0.0	8.184062847055415E-5
3	0.0	0.0	0.0	0.0	8.184062847055415E-5
4	0.0	0.0	0.0	0.0	8.184062847055415E-5
5	0.0	0.0	0.0	0.0	1.2276094270583123E-4
6	0.0	0.0	0.0	0.0	1.2276094270583123E-4
7	0.0	0.0	0.0	0.0	1.2276094270583123E-4
8	0.0	0.0	0.0	0.0	1.2276094270583123E-4
9	0.0	0.0	0.0	4.0920314235277076E-5	1.2276094270583123E-4
10	0.0	0.0	0.0	4.0920314235277076E-5	1.2276094270583123E-4
11	0.0	0.0	0.0	4.0920314235277076E-5	2.0460157117638538E-4
12	0.0	0.0	0.0	4.0920314235277076E-5	7.774859704702644E-4
13	0.0	0.0	0.0	4.0920314235277076E-5	9.820875416466498E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGA	220	0.0	14.682547	16
GGTATCA	1145	0.0	14.265018	1
GTTCGCG	60	2.5682439E-5	14.250707	16
CGTTCGC	80	1.9991603E-6	13.063416	15
ACGAACG	250	0.0	12.920906	15
TTCTACG	105	2.0008883E-8	12.664181	2
CGTTATT	335	0.0	12.4751625	2
GCGTTAT	345	0.0	12.386336	1
GTATCAA	3010	0.0	12.335583	1
TTCGCGC	70	1.09193075E-4	12.213641	17
AATCGTA	55	0.0030676972	12.091757	13
ATAACGA	280	0.0	11.876076	12
TAACGAA	280	0.0	11.875833	13
ACTCTAA	475	0.0	11.801794	10
GTATTAT	105	2.736324E-7	11.757188	1
TCGCGCA	65	8.0326176E-4	11.691211	18
AACGAAC	280	0.0	11.536523	14
CGACCAT	685	0.0	11.5127	10
CGATAAC	290	0.0	11.467261	10
CTACTAA	75	2.0726E-4	11.401267	9
>>END_MODULE