FastQCFastQC Report
Thu 2 Feb 2017
SRR4062181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2443774
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT47140.19289836130509613No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC40860.16720040396534214No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA40160.16433598196887272No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG39050.159793827088757No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT39030.15971198646028642No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT38630.15807517389087533No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA36140.14788601564629136No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA34630.14170704819676452No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC30500.12480695841759508No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC29940.12251542082041957No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT29780.12186069579265514No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC29740.12169701453571403No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA29610.12116505045065543No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT28780.11776866436912742No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG28210.11543620645771663No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC26750.10946184057936618No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA25690.1051242872704268No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT25150.10291459030172184No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT24700.10107317616113438No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG24560.1005002917618405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12500.027.4655781
GTATCAA30800.026.795061
GTATAGG2850.022.3937741
CGCAATA7750.019.02015136
TATAACG708.1253966E-418.8575482
TAGCGGC8350.016.86194630
TCAACGC49200.016.8123364
ATCAACG49500.016.7104423
CGAGCCG12850.016.60702115
ACCGTCG10100.016.5537938
TCTAGCG8650.016.5314728
CGGTCCA9050.016.52972610
CAACGCA50350.016.4720335
CAATACG8800.016.25101338
AACGCAG50900.016.2508246
ATACCGT10700.016.242346
AGAGCGA16350.016.14680515
TACCGTC10650.016.1120327
GTATAGA2051.05501385E-1016.1031781
GCGAAAG16300.016.06136518
Produced by FastQC (version 0.11.5)