##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062181_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2443774
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.301135047676258	32.0	32.0	32.0	32.0	32.0
2	31.47201009585993	32.0	32.0	32.0	32.0	32.0
3	31.58221218492381	32.0	32.0	32.0	32.0	32.0
4	31.654361655374025	32.0	32.0	32.0	32.0	32.0
5	31.626589447305683	32.0	32.0	32.0	32.0	32.0
6	35.27395618416433	36.0	36.0	36.0	36.0	36.0
7	35.27185124320007	36.0	36.0	36.0	36.0	36.0
8	35.22437017498345	36.0	36.0	36.0	36.0	36.0
9	35.31027050782929	36.0	36.0	36.0	36.0	36.0
10	35.18728163897316	36.0	36.0	36.0	36.0	36.0
11	35.30994150850283	36.0	36.0	36.0	36.0	36.0
12	35.22643501403976	36.0	36.0	36.0	36.0	36.0
13	35.27053156306598	36.0	36.0	36.0	36.0	36.0
14	35.22289827128041	36.0	36.0	36.0	36.0	36.0
15	35.2074152519832	36.0	36.0	36.0	36.0	36.0
16	35.21489875905055	36.0	36.0	36.0	36.0	36.0
17	35.18801165737912	36.0	36.0	36.0	36.0	36.0
18	35.19068129867983	36.0	36.0	36.0	36.0	36.0
19	35.1831941087842	36.0	36.0	36.0	36.0	36.0
20	35.177749660975195	36.0	36.0	36.0	36.0	36.0
21	35.17396043987701	36.0	36.0	36.0	36.0	36.0
22	35.161576315976845	36.0	36.0	36.0	36.0	36.0
23	35.12411867873216	36.0	36.0	36.0	36.0	36.0
24	35.103943327001595	36.0	36.0	36.0	36.0	36.0
25	35.08957415865788	36.0	36.0	36.0	36.0	36.0
26	35.04971040693616	36.0	36.0	36.0	36.0	36.0
27	35.0320442888745	36.0	36.0	36.0	36.0	36.0
28	35.017279421092134	36.0	36.0	36.0	36.0	36.0
29	35.000483678114264	36.0	36.0	36.0	36.0	36.0
30	34.98319975578757	36.0	36.0	36.0	36.0	36.0
31	34.98870353805221	36.0	36.0	36.0	36.0	36.0
32	34.95991937061283	36.0	36.0	36.0	36.0	36.0
33	34.939397014617555	36.0	36.0	36.0	32.0	36.0
34	34.92696828757487	36.0	36.0	36.0	36.0	36.0
35	34.91628153830919	36.0	36.0	36.0	36.0	36.0
36	34.893291687365526	36.0	36.0	36.0	32.0	36.0
37	34.88616091340688	36.0	36.0	36.0	32.0	36.0
38	34.865224034628405	36.0	36.0	36.0	32.0	36.0
39	34.85573747817924	36.0	36.0	36.0	32.0	36.0
40	34.8396414725748	36.0	36.0	36.0	32.0	36.0
41	34.81363088403428	36.0	36.0	36.0	32.0	36.0
42	34.777713487417415	36.0	36.0	36.0	32.0	36.0
43	34.78164511120914	36.0	36.0	36.0	32.0	36.0
44	34.72040376892462	36.0	36.0	36.0	32.0	36.0
45	34.72028059877877	36.0	36.0	36.0	32.0	36.0
46	34.700609385319595	36.0	36.0	36.0	32.0	36.0
47	34.685126775225534	36.0	36.0	36.0	32.0	36.0
48	34.65144853820362	36.0	36.0	36.0	32.0	36.0
49	34.647867192301746	36.0	36.0	36.0	32.0	36.0
50	34.21851979765723	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	13.0
20	27.0
21	64.0
22	146.0
23	421.0
24	1039.0
25	2607.0
26	5638.0
27	10895.0
28	18429.0
29	29689.0
30	44750.0
31	66069.0
32	100954.0
33	173758.0
34	402362.0
35	1586911.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.45148954825421	17.674005081480104	11.895059699409208	25.979445670856478
2	16.892181941617974	19.45706097772074	35.97734221126235	27.673414869398933
3	18.771552279738614	22.579484869056664	27.85449676667211	30.79446608453261
4	12.856958131152881	15.487479611453434	34.268348873504664	37.38721338388902
5	15.518415369015301	35.84046642610978	32.15866115279072	16.482457052084197
6	36.386276466758574	34.06310651603074	15.743769981909123	13.806847035301562
7	32.078089054061465	29.43148589026645	19.343155299958177	19.147269755713907
8	29.190751681620313	32.136154979961326	18.352965536092945	20.32012780232542
9	27.12366200802529	14.151267670414693	18.370233908700232	40.35483641285978
10	17.489452771742023	25.53722322477156	29.21003204065849	27.763291962827925
11	38.39082501082342	20.76910549011488	21.013277005156777	19.826792493904918
12	25.168653075120694	23.248385489001848	27.245277181932536	24.33768425394492
13	29.410125486235632	18.556339497842274	25.008777407403464	27.02475760851863
14	24.372507441359144	19.27113554690409	23.70644748655154	32.64990952518523
15	25.910579292520502	26.49181143591838	21.188784232911882	26.408825038649237
16	27.12668531555524	25.14129888581348	22.389960372475812	25.342055426155476
17	24.887162233496223	25.65499100980696	24.00569774455412	25.452149012142694
18	25.94962944533628	24.128712656556328	25.159497481144903	24.762160416962487
19	26.264212648141765	24.4073715490876	24.283178395383533	25.0452374073871
20	26.593757429609756	23.546988279170414	23.722127582435164	26.13712670878467
21	27.926980124586038	23.441088840902978	23.482131932875927	25.149799101635057
22	27.09972362419765	23.282881313902184	24.29308111142847	25.324313950471687
23	25.01524281705264	23.569937318262653	24.9308651290995	26.483954735585208
24	25.455539455533728	24.311805908212417	24.556757214626206	25.675897421627653
25	25.696680575521313	23.66547219927268	24.406991337553865	26.230855887652137
26	25.342576463082928	25.09709419210798	24.76175043304153	24.798578911767557
27	26.055429998031727	23.927567562701356	24.274081776208618	25.742920663058307
28	25.489260514318428	23.672863591406447	25.133862495135528	25.704013399139598
29	25.082925288264097	24.093059646463697	25.181420807065813	25.64259425820639
30	25.1375248031604	24.325040992478336	25.269453677783364	25.267980526577897
31	26.03686617087289	24.05189057181603	23.912474148422223	25.99876910888885
32	25.92309936650665	23.874731918268267	23.77218464565334	26.429984069571738
33	25.242637797563265	23.866319443023595	24.3888735664544	26.502169192958736
34	26.252437847672066	23.956871272640374	24.54567849300707	25.245012386680493
35	26.71008599022983	23.41067025238914	24.90754024730682	24.971703510074207
36	24.946353248513358	24.50829949209498	24.264045136928118	26.281302122463547
37	26.56190856172442	24.09477334806494	24.22903559115694	25.114282499053697
38	25.485990112015266	23.556924658335838	24.757117474856514	26.19996775479238
39	26.487647303865604	23.681436541588816	23.759594853500225	26.07132130104536
40	26.40307688278612	24.082115680186202	24.435217360703042	25.07959007632463
41	25.055682978379494	24.83421960965649	24.908531782162214	25.2015656298018
42	26.845102295955424	24.455475324770987	24.229624188456178	24.469798190817407
43	26.153474705973828	23.446346873132924	24.401798940932864	25.998379479960388
44	25.449887034618847	24.21052114668558	24.10809319920169	26.23149861949388
45	25.769279245215078	24.412902317617924	24.200029790695808	25.617788646471197
46	25.503578987342184	24.382127488226907	24.1706865141007	25.943607010330204
47	25.705972980106434	24.04500451149972	24.713402120916378	25.53562038747747
48	26.736430387485743	24.7437775123308	23.28544238866266	25.234349711520792
49	25.49962413353265	24.892222285423625	23.787129857465377	25.821023723578346
50	25.886817653938355	24.75498600913846	23.774520836521752	25.58367550040143
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	27.0
1	33.5
2	40.0
3	118.0
4	196.0
5	222.0
6	248.0
7	200.0
8	152.0
9	229.5
10	307.0
11	520.5
12	734.0
13	1376.5
14	2019.0
15	2574.0
16	3129.0
17	3272.5
18	3416.0
19	3381.5
20	3347.0
21	3993.0
22	4639.0
23	5295.5
24	5952.0
25	8076.0
26	10200.0
27	13245.5
28	16291.0
29	18786.0
30	21281.0
31	23519.5
32	25758.0
33	30285.0
34	34812.0
35	41344.5
36	47877.0
37	61976.0
38	76075.0
39	85503.5
40	94932.0
41	105326.0
42	115720.0
43	118502.0
44	121284.0
45	135490.5
46	149697.0
47	163476.5
48	177256.0
49	187466.5
50	197677.0
51	188983.0
52	180289.0
53	183437.5
54	186586.0
55	192015.0
56	197444.0
57	192619.5
58	187795.0
59	174082.0
60	160369.0
61	141035.5
62	121702.0
63	105128.5
64	88555.0
65	75927.5
66	63300.0
67	55335.0
68	47370.0
69	42349.5
70	37329.0
71	28865.0
72	20401.0
73	18262.5
74	16124.0
75	11727.0
76	7330.0
77	6623.5
78	5917.0
79	5055.5
80	4194.0
81	3375.0
82	2556.0
83	2187.0
84	1818.0
85	1402.5
86	987.0
87	688.0
88	389.0
89	241.0
90	93.0
91	72.0
92	51.0
93	41.5
94	32.0
95	35.0
96	38.0
97	33.5
98	29.0
99	19.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0321224466746925
2	0.004542154880115755
3	2.8644219964693953E-4
4	0.0
5	0.0
6	4.0920314235277076E-5
7	0.0
8	0.0
9	0.0
10	1.636812569411083E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.365656562349874E-4
17	0.0
18	2.0460157117638538E-4
19	0.0
20	2.0460157117638538E-4
21	4.0920314235277076E-5
22	0.0
23	0.0
24	9.411672274113727E-4
25	2.8644219964693953E-4
26	5.31964085058602E-4
27	2.8644219964693953E-4
28	0.0014322109982346978
29	3.273625138822166E-4
30	0.0013503703697641436
31	6.547250277644332E-4
32	9.411672274113727E-4
33	3.273625138822166E-4
34	8.184062847055415E-4
35	3.273625138822166E-4
36	4.0920314235277076E-4
37	0.0020050953975285766
38	0.0
39	6.547250277644332E-4
40	8.184062847055415E-5
41	0.001186689112823035
42	0.005278720536350743
43	0.004664915822821587
44	0.004296632994704093
45	0.00237337822564607
46	0.0027007407395282872
47	7.774859704702643E-4
48	0.003969270480821877
49	0.0035600673384691057
50	0.0014731313124699747
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2443774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.39233183372805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.13909427287277	28.330815703870115
2	15.549746113063033	12.561810098581299
3	5.712083735644349	6.921731451365624
4	2.659752157215447	4.297343669188814
5	1.4770093277357046	2.982992544370608
6	0.9241939600965113	2.2398209468967303
7	0.6336373623723297	1.7915863422233933
8	0.46001884323004305	1.4864987012412496
9	0.33828445279863995	1.22976880844704
>10	1.8483970011734883	13.483396454362659
>50	0.12985118124740708	3.6268131889502495
>100	0.10112822913688421	8.699568447464026
>500	0.016102400833940878	4.554870564430596
>1k	0.010700962579517649	7.79298307860775
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	4714	0.19289836130509613	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	4086	0.16720040396534214	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	4016	0.16433598196887272	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	3905	0.159793827088757	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3903	0.15971198646028642	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	3863	0.15807517389087533	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	3614	0.14788601564629136	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	3463	0.14170704819676452	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	3050	0.12480695841759508	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2994	0.12251542082041957	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	2978	0.12186069579265514	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC	2974	0.12169701453571403	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2961	0.12116505045065543	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	2878	0.11776866436912742	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG	2821	0.11543620645771663	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC	2675	0.10946184057936618	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	2569	0.1051242872704268	No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT	2515	0.10291459030172184	No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT	2470	0.10107317616113438	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	2456	0.1005002917618405	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2276094270583123E-4
2	0.0	0.0	0.0	0.0	1.2276094270583123E-4
3	0.0	0.0	0.0	0.0	1.2276094270583123E-4
4	0.0	0.0	0.0	0.0	1.2276094270583123E-4
5	0.0	0.0	0.0	0.0	1.2276094270583123E-4
6	0.0	0.0	0.0	0.0	2.0460157117638538E-4
7	0.0	0.0	0.0	0.0	2.0460157117638538E-4
8	0.0	0.0	0.0	0.0	2.0460157117638538E-4
9	0.0	0.0	0.0	0.0	2.0460157117638538E-4
10	0.0	0.0	0.0	0.0	2.0460157117638538E-4
11	0.0	0.0	0.0	4.0920314235277076E-5	2.4552188541166245E-4
12	0.0	0.0	0.0	4.0920314235277076E-5	5.728843992938791E-4
13	0.0	0.0	0.0	4.0920314235277076E-5	8.184062847055415E-4
14	0.0	0.0	0.0	4.0920314235277076E-5	8.184062847055415E-4
15	0.0	0.0	0.0	8.184062847055415E-5	9.820875416466498E-4
16	0.0	0.0	0.0	1.636812569411083E-4	0.0010230078558819268
17	0.0	0.0	0.0	1.636812569411083E-4	0.0010230078558819268
18	0.0	0.0	0.0	2.0460157117638538E-4	0.0010230078558819268
19	0.0	0.0	0.0	2.0460157117638538E-4	0.001063928170117204
20	0.0	0.0	0.0	3.273625138822166E-4	0.0013094500555288664
21	0.0	0.0	0.0	4.0920314235277076E-4	0.0013503703697641434
22	0.0	0.0	0.0	6.956453419997103E-4	0.0014731313124699747
23	0.0	0.0	0.0	0.0010230078558819268	0.0014731313124699747
24	0.0	0.0	0.0	0.0015140516267052517	0.0015140516267052517
25	0.0	0.0	0.0	0.0018414141405874683	0.0015140516267052517
26	0.0	0.0	0.0	0.002086936025999131	0.001636812569411083
27	0.0	0.0	0.0	0.0024961391683519015	0.001636812569411083
28	0.0	0.0	0.0	0.002864421996469395	0.001636812569411083
29	0.0	0.0	0.0	0.0036419079669396596	0.001636812569411083
30	0.0	0.0	0.0	0.004705836137056864	0.001636812569411083
31	0.0	0.0	0.0	0.007570258133526259	0.0017186531978816372
32	0.0	0.0	0.0	0.01063928170117204	0.0017186531978816372
33	0.0	0.0	0.0	0.013667384954582543	0.0017186531978816372
34	0.0	0.0	0.0	0.01775941637811025	0.0017186531978816372
35	0.0	0.0	0.0	0.022178810315520176	0.0017186531978816372
36	0.0	0.0	0.0	0.027784893365753134	0.0017186531978816372
37	0.0	0.0	0.0	0.0364190796693966	0.0017595735121169141
38	0.0	0.0	0.0	0.04718112231327447	0.0018004938263521913
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1250	0.0	27.465578	1
GTATCAA	3080	0.0	26.79506	1
GTATAGG	285	0.0	22.393774	1
CGCAATA	775	0.0	19.020151	36
TATAACG	70	8.1253966E-4	18.857548	2
TAGCGGC	835	0.0	16.861946	30
TCAACGC	4920	0.0	16.812336	4
ATCAACG	4950	0.0	16.710442	3
CGAGCCG	1285	0.0	16.607021	15
ACCGTCG	1010	0.0	16.553793	8
TCTAGCG	865	0.0	16.53147	28
CGGTCCA	905	0.0	16.529726	10
CAACGCA	5035	0.0	16.472033	5
CAATACG	880	0.0	16.251013	38
AACGCAG	5090	0.0	16.250824	6
ATACCGT	1070	0.0	16.24234	6
AGAGCGA	1635	0.0	16.146805	15
TACCGTC	1065	0.0	16.112032	7
GTATAGA	205	1.05501385E-10	16.103178	1
GCGAAAG	1630	0.0	16.061365	18
>>END_MODULE