FastQCFastQC Report
Thu 2 Feb 2017
SRR4062180_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062180_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242265
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35070.28230691519120316No Hit
TATCAACGCAGAGTACTTTTTTTTT20690.1665506152069003No Hit
GGTATCAACGCAGAGTACTTTTTTT13210.1063380196656913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGT400.00527600514.25093812
ATTAGGC400.005278827614.2497893
CCGTCGT1051.366061E-913.5739629
CGCATCG1251.0186341E-1012.91980813
CTAATAG604.0915044E-412.6664783
TGTACGC604.0942876E-412.6654582
TAGGACA1900.012.4998144
CGGACAT1155.30963E-912.3926195
TGTAGGA3900.012.1783262
CGTCGTA1353.6925485E-1011.96519710
TCTAGAC802.8657028E-511.8748253
CGCCAGT1459.822543E-1111.79150318
GCCTTAT658.044148E-411.6883651
GGTATCA8400.011.6448811
CATCGCC1657.2759576E-1211.5149815
GCGACGA1001.9287E-611.39983113
TCGCCAG1501.7644197E-1011.39845217
GATCTAT752.0796768E-411.3961561
GTCTATA1104.970625E-711.2262012
GCACCGC600.00586812911.0849566
Produced by FastQC (version 0.11.5)