##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062180_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1242265
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23176697403533	32.0	32.0	32.0	32.0	32.0
2	30.797469139032334	32.0	32.0	32.0	32.0	32.0
3	30.826538620986664	32.0	32.0	32.0	32.0	32.0
4	30.827210780308548	32.0	32.0	32.0	32.0	32.0
5	30.77355958672264	32.0	32.0	32.0	32.0	32.0
6	34.41674602439898	36.0	36.0	36.0	32.0	36.0
7	34.33599513791341	36.0	36.0	36.0	32.0	36.0
8	34.29565913875059	36.0	36.0	36.0	32.0	36.0
9	34.40749759511859	36.0	36.0	36.0	32.0	36.0
10	34.11638418533888	36.0	36.0	36.0	32.0	36.0
11	34.41991282053346	36.0	36.0	36.0	32.0	36.0
12	34.23986025525954	36.0	36.0	36.0	32.0	36.0
13	34.32906907946372	36.0	36.0	36.0	32.0	36.0
14	34.21657214845464	36.0	36.0	36.0	32.0	36.0
15	34.18618249729325	36.0	36.0	36.0	32.0	36.0
16	34.195925185045056	36.0	36.0	36.0	32.0	36.0
17	34.127657142397155	36.0	36.0	36.0	32.0	36.0
18	34.14231987538891	36.0	36.0	36.0	32.0	36.0
19	34.13736119105022	36.0	36.0	36.0	32.0	36.0
20	34.110334751441926	36.0	36.0	36.0	32.0	36.0
21	34.08298229443798	36.0	36.0	36.0	32.0	36.0
22	34.056848579006896	36.0	36.0	36.0	32.0	36.0
23	34.03309036316728	36.0	36.0	36.0	32.0	36.0
24	34.001466675789786	36.0	36.0	36.0	32.0	36.0
25	33.66649064410573	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	19.0
5	100.0
6	245.0
7	57.0
8	204.0
9	155.0
10	88.0
11	27.0
12	36.0
13	62.0
14	251.0
15	343.0
16	478.0
17	624.0
18	902.0
19	1295.0
20	1788.0
21	2633.0
22	3715.0
23	5540.0
24	7857.0
25	10821.0
26	14618.0
27	18427.0
28	24242.0
29	31574.0
30	41232.0
31	56295.0
32	80093.0
33	113857.0
34	253345.0
35	571341.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.14881819660069	17.34025408652337	11.86488431512396	25.646043401751978
2	16.267341349892373	19.64827065466061	38.35905791209906	25.72533008334796
3	19.279826960121778	23.363362009632468	28.300424147338273	29.056386882907482
4	12.842682870867941	15.71367962085003	35.69883435696419	35.74480315131784
5	14.642497368846987	36.610913869699345	33.55925952469338	15.187329236760288
6	34.131733150762166	35.07114234509977	16.925313626386902	13.871810877751154
7	30.186214682345586	30.59169838201367	20.18200029492654	19.040086640714204
8	27.762252413415204	33.56492449515706	19.234556560137957	19.438266531289788
9	27.13277876545518	14.868968429603674	18.705866803777425	39.29238600116372
10	16.218810754345295	26.920361229418084	31.381080270696376	25.479747745540248
11	36.217771741101835	21.662387702377544	22.71731025782607	19.402530298694547
12	24.680718672144323	23.66909356746357	28.994976129967753	22.655211630424358
13	29.311042406813975	20.21891703687465	25.201894992037037	25.26814556427434
14	23.172749376041107	19.999564820403798	25.68631448817641	31.141371315378684
15	24.663359776631776	27.851870241375032	22.494079678102143	24.99069030389105
16	24.893761912245285	26.440449854736087	24.372423630671033	24.293364602347594
17	23.422798994820976	26.473878401707324	25.67107305601181	24.432249547459893
18	23.762055493952914	25.74403994429975	26.75803914489801	23.735865416849325
19	25.145172583552288	25.35103685494183	25.897564194603373	23.606226366902504
20	25.435269169971303	24.89820220337645	25.705736287711918	23.960792338940337
21	25.701705266465506	24.69852677546318	25.26872147314397	24.331046484927345
22	25.193952110669542	24.97735748105603	25.596038123233317	24.232652285041112
23	24.142328548284578	25.130649635018855	25.77183381575884	24.955188000937728
24	24.17734348940751	25.224990774773964	26.114560387765657	24.483105348052874
25	24.379732822859186	25.121515566802742	25.96489362302097	24.533857987317102
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	573.0
1	573.0
2	576.0
3	579.0
4	579.0
5	579.0
6	1246.0
7	1913.0
8	1913.0
9	1913.0
10	2186.5
11	2460.0
12	2460.0
13	2460.0
14	3192.0
15	3924.0
16	3924.0
17	3924.0
18	6534.0
19	9144.0
20	9144.0
21	9144.0
22	14342.5
23	19541.0
24	19541.0
25	19541.0
26	28844.5
27	38148.0
28	38148.0
29	38148.0
30	49147.5
31	60147.0
32	60147.0
33	60147.0
34	71122.0
35	82097.0
36	82097.0
37	82097.0
38	94632.0
39	107167.0
40	107167.0
41	107167.0
42	120666.5
43	134166.0
44	134166.0
45	134166.0
46	146137.0
47	158108.0
48	158108.0
49	158108.0
50	162692.0
51	167276.0
52	167276.0
53	167276.0
54	155718.5
55	144161.0
56	144161.0
57	144161.0
58	130497.5
59	116834.0
60	116834.0
61	116834.0
62	101594.5
63	86355.0
64	86355.0
65	86355.0
66	70145.0
67	53935.0
68	53935.0
69	53935.0
70	40993.0
71	28051.0
72	28051.0
73	28051.0
74	21262.5
75	14474.0
76	14474.0
77	14474.0
78	11059.0
79	7644.0
80	7644.0
81	7644.0
82	5347.0
83	3050.0
84	3050.0
85	3050.0
86	2216.0
87	1382.0
88	1382.0
89	1382.0
90	937.5
91	493.0
92	493.0
93	493.0
94	319.0
95	145.0
96	145.0
97	145.0
98	321.5
99	498.0
100	498.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.8547934619425E-4
2	2.4149436714388634E-4
3	6.439849790503637E-4
4	0.009498778440992865
5	0.03332622266585632
6	0.05956861056215863
7	0.1028766004032956
8	0.10344008725996466
9	0.11422683565905825
10	0.1298434714010296
11	0.1368468080482023
12	0.12783101834149718
13	0.1224376441419504
14	0.11253637508905105
15	0.1306484526248425
16	0.11430733378143955
17	0.12002270047051153
18	0.10762598962379202
19	0.09305582947277755
20	0.08685747404941781
21	0.08935291584323796
22	0.09957617738566248
23	0.07792218246509401
24	0.08878942898656889
25	0.08581099845846096
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1242265.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.42754980328436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.48599710612302	39.996627673375215
2	15.004536079126757	16.33320269443697
3	5.155806740554645	8.418537844429414
4	2.231148501046052	4.857437846368288
5	1.148204091320265	3.1246967682334286
6	0.6958959485188356	2.2725546837547643
7	0.43586365875094046	1.6606093695875987
8	0.3087383594590835	1.3443097948514862
9	0.22940003946204648	1.1237113865426338
>10	1.1801586275533051	11.398804249596466
>50	0.07346550177965164	2.745102782246233
>100	0.04647899670054603	4.834542003563261
>500	0.0037123703491127	1.2483779840037246
>1k	5.93979255858032E-4	0.6414849190105893
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3507	0.28230691519120316	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2069	0.1665506152069003	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1321	0.1063380196656913	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6099624476259092E-4
2	0.0	0.0	0.0	0.0	2.4149436714388637E-4
3	0.0	0.0	0.0	0.0	2.4149436714388637E-4
4	0.0	0.0	0.0	0.0	2.4149436714388637E-4
5	0.0	0.0	0.0	0.0	2.4149436714388637E-4
6	0.0	0.0	0.0	0.0	2.4149436714388637E-4
7	0.0	0.0	0.0	0.0	2.4149436714388637E-4
8	0.0	0.0	0.0	0.0	2.4149436714388637E-4
9	0.0	0.0	0.0	0.0	2.4149436714388637E-4
10	0.0	0.0	0.0	0.0	2.4149436714388637E-4
11	0.0	0.0	0.0	0.0	2.4149436714388637E-4
12	0.0	0.0	0.0	0.0	5.634868566690682E-4
13	0.0	0.0	0.0	0.0	5.634868566690682E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTGGT	40	0.005276005	14.250938	12
ATTAGGC	40	0.0052788276	14.249789	3
CCGTCGT	105	1.366061E-9	13.573962	9
CGCATCG	125	1.0186341E-10	12.919808	13
CTAATAG	60	4.0915044E-4	12.666478	3
TGTACGC	60	4.0942876E-4	12.665458	2
TAGGACA	190	0.0	12.499814	4
CGGACAT	115	5.30963E-9	12.392619	5
TGTAGGA	390	0.0	12.178326	2
CGTCGTA	135	3.6925485E-10	11.965197	10
TCTAGAC	80	2.8657028E-5	11.874825	3
CGCCAGT	145	9.822543E-11	11.791503	18
GCCTTAT	65	8.044148E-4	11.688365	1
GGTATCA	840	0.0	11.644881	1
CATCGCC	165	7.2759576E-12	11.51498	15
GCGACGA	100	1.9287E-6	11.399831	13
TCGCCAG	150	1.7644197E-10	11.398452	17
GATCTAT	75	2.0796768E-4	11.396156	1
GTCTATA	110	4.970625E-7	11.226201	2
GCACCGC	60	0.005868129	11.084956	6
>>END_MODULE